{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T18:56:11Z","timestamp":1774637771512,"version":"3.50.1"},"reference-count":63,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T00:00:00Z","timestamp":1770854400000},"content-version":"vor","delay-in-days":42,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This study aimed to evaluate the effects of supplementing two vitamin D sources on reproductive performance, egg quality, and plasma biochemical indices of aged duck breeders, and to estimate vitamin D requirements and relative bioavailability from these two sources. A total of 180 laying Pekin ducks (64 wk of age) were randomly assigned to 9 different treatments, each containing 10 replicates with 2 birds per replicate. The birds were fed a basal diet with no vitamin D supplementation or supplemented with cholecalciferol (VD3) or 25-hydroxycholecalciferol (25-OH-D3) at 250, 500, 1000, or 2000\u2009IU\/kg of feed for 15 wk. The two-way ANOVA (2\u2009\u00d7\u20094 factors, without a control group) and one-way ANOVA were employed to compare the differences between 25-OH-D3 and VD3. In comparison to ducks fed the basal diet, the egg production (9 to 15 wks), ovarian weight, and the number and weight of dominant follicles increased with increasing VD3 or 25-OH-D3 levels (linear and quadratic, P\u2009&amp;lt;\u20090.05). The egg Haugh unit exhibited a linear increase with rising VD3 levels (P\u2009=\u20090.021) and a quadratic increase with rising 25-OH-D3 levels (P\u2009=\u20090.042). Additionally, the plasma calcium content increased linearly as dietary 25-OH-D3 levels increased (P\u2009=\u20090.001). Furthermore, diets supplemented with 25-OH-D3 resulted in higher plasma 25-OH-D3 concentrations compared to those fed VD3 (P\u2009&amp;lt;\u20090.001). According to the quadratic broken-line model, the VD3 requirements of duck breeders in terms of enhancing egg production, ovarian weight, and dominant follicle number were 906, 359, and 730\u2009IU\/kg, respectively, whereas the 25-OH-D3 requirements were 260, 324, and 308\u2009IU\/kg, respectively. Based on slope ratio comparison from multiple linear regressions of ovarian weight, dominant follicle number, and plasma 25-OH-D3 concentration, the bioavailability of 25-OH-D3 were 147%, 191%, and 211%, respectively, relative to VD3.<\/jats:p>","DOI":"10.1093\/jas\/skag037","type":"journal-article","created":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T12:40:00Z","timestamp":1770295200000},"source":"Crossref","is-referenced-by-count":0,"title":["Dietary supplementation with 25-hydroxycholecalciferol is more effective than cholecalciferol in improving reproductive performance in aged duck breeders"],"prefix":"10.1093","volume":"104","author":[{"given":"Yating","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences , Beijing, 100193,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongyan","family":"Jin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences , Beijing, 100193,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4610-5188","authenticated-orcid":false,"given":"Lei","family":"Zhuang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences , Beijing, 100193,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences , Beijing, 100193,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuaiqin","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences , Beijing, 100193,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jindang","family":"Cao","sequence":"additional","affiliation":[{"name":"Shandong Haineng Bioengineering Co., Ltd , Rizhao, 276800,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingkai","family":"Wang","sequence":"additional","affiliation":[{"name":"Shandong Haineng Bioengineering Co., Ltd , Rizhao, 276800,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Chen","sequence":"additional","affiliation":[{"name":"Food Science and Engineering College, Beijing University of Agriculture , Beijing, 102206,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiannan","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences , Beijing, 100193,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5192-2465","authenticated-orcid":false,"given":"Zhengkui","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences , Beijing, 100193,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences , Beijing, 100193,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuisheng","family":"Hou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences , Beijing, 100193,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7653-8438","authenticated-orcid":false,"given":"Jing","family":"Tang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences , Beijing, 100193,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2026,2,12]]},"reference":[{"key":"2026032713570829600_skag037-B1","first-page":"1774","article-title":"Impact of vitamin D deficiency on reproductive outcome in infertile anovulatory women with 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