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Microbial tryptophan catabolites play a vital role in maintaining the health of the intestinal mucosa. Indole-3-acetic acid (IAA), an indole derivative with known anti-inflammatory properties, has not yet been studied for its impact on piglets\u2019 intestinal health. Twenty-four weaned crossbred piglets (Duroc \u00d7 Yorkshire \u00d7 Landrace, weighing 6.58\u2009\u00b1\u20090.07\u2009kg) were randomly allocated to receive diets containing 0, 120, or 240\u2009mg\/kg indole-3-acetate sodium (IAA-Na). Although dietary IAA-Na did not significantly impact growth performance or diarrhea incidence (P\u2009&amp;gt;\u20090.05), the 240\u2009mg\/kg IAA-Na elevated jejunal villus width (P\u2009&amp;lt;\u20090.05), tended to increase villus surface area (P\u2009&amp;lt;\u20090.10), and enhanced apparent nutrient digestibility alongside upregulating the mRNA expression of transporters (P\u2009&amp;lt;\u20090.05). Furthermore, dietary IAA-Na promoted intestinal epithelial cell proliferation and reduced secretory cell numbers (P\u2009&amp;lt;\u20090.05). Transcriptomic analysis of the jejunum of the 240\u2009mg\/kg group revealed significant modulation of pathways related to the \u201cinflammatory response\u201d and \u201cimmune system processes.\u201d Consistent with this, dietary supplementation with 240\u2009mg\/kg IAA-Na downregulated the content and expression of proinflammatory cytokines while upregulating the content and expression of anti-inflammatory cytokines in the jejunum. To further elucidate the effect of IAA on epithelial renewal, piglet jejunal organoids were employed as an in vitro model. Treatment with 0.5 and 2\u2009\u03bcM IAA-Na significantly increased the organoids budding rate on d 3 (P\u2009&amp;lt;\u20090.01), indicating enhanced epithelial renewal capacity. In conclusion, although dietary IAA did not improve overall growth performance, 240\u2009mg\/kg IAA-Na promoted nutrient absorption in weaned piglets, potentially through enhancing anti-inflammatory responses and epithelial renewal.<\/jats:p>","DOI":"10.1093\/jas\/skag048","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T12:51:30Z","timestamp":1770900690000},"source":"Crossref","is-referenced-by-count":0,"title":["Effects of dietary indole-3-acetate sodium on intestinal morphology, nutrient absorption, and inflammatory responses in weaned piglets"],"prefix":"10.1093","volume":"104","author":[{"given":"Lanmei","family":"Yin","sequence":"first","affiliation":[{"name":"Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University , Changsha, 410081,","place":["China"]},{"name":"Key Laboratory of Agro-Ecological Processes in Subtropical 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