{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T17:48:28Z","timestamp":1782323308783,"version":"3.54.5"},"reference-count":55,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T00:00:00Z","timestamp":1778284800000},"content-version":"vor","delay-in-days":128,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Foundation for Food and Agriculture Research and International Consortium for Antimicrobial Stewardship in Agriculture","award":["ICASALAWG-064"],"award-info":[{"award-number":["ICASALAWG-064"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This study evaluated the main and interactive effects of dietary starch concentration (49.1% [control] or 64.4% [high starch]) and feeding management regimen (regular [REG] or erratic [ERR]) on prevalence of Fusobacterium necrophorum subsp. necrophorum, F. necrophorum subsp. funduliforme, Trueperella pyogenes, and Salmonella enterica in liver abscesses (LA) and ruminal and colonic tissues of finishing beef steers. Intact LA (n\u2009=\u2009132) and sections of ruminal (n\u2009=\u2009131) and colonic (n\u2009=\u2009120) epithelial tissues were collected from steers at slaughter. Samples were cultured, before and after enrichment, to determine prevalence of four bacterial pathogens and quantitative PCR (qPCR) was used to determine prevalence and concentrations of the two subspecies of F. necrophorum. Subsp. necrophorum was the dominant pathogen in LA, whereas subsp. funduliforme was dominant in ruminal and colonic tissues by both culture and qPCR methods. Steers fed the high-starch diet had greater prevalence (P\u2009=\u20090.01) of subsp. necrophorum, T. pyogenes, and S. enterica in LA compared to steers fed the control diet, indicating the potential impact of dietary starch on promoting bacterial translocation. Of the 132 LA analyzed, culture and qPCR identified 21.7% and 16.4% of LA, respectively, to have subsp. funduliforme, without the subsp. necrophorum, suggesting that subsp. funduliforme without subsp. necrophorum may have caused LA. Concentrations of F. necrophorum in LA were between 7.0 to 7.5 log10 CFU per g, were 2 to 3 log less in ruminal and colonic epithelial tissues, and were not affected by the diet (P\u2009\u2265\u20090.08). The prevalence of S. enterica was greater (P\u2009&amp;lt;\u20090.01) in colonic (71.9%) than ruminal epithelial tissues (48.3%), suggesting that the colon may be the major source of the Salmonella in LA. A total of eight serotypes were identified, and the majority (88.6%) belonged to Kentucky (27.2%), Montevideo (26.2%), Anatum (23.3%), and Lubbock (11.9%). Feeding management regimen had minimal effect, however, diet affected prevalence of the pathogens in LA but not ruminal and colonic epithelial tissues. The prevalence and concentrations of both subspecies of F. necrophorum in colonic epithelial tissue suggest the possibility of the colon being a source of LA pathogens. However, the prevalence and concentration of F. necrophorum in colonic epithelial tissues were much less than that of ruminal tissues, suggesting that the rumen may be the primary reservoir of F. necrophorum in LA.<\/jats:p>","DOI":"10.1093\/jas\/skag131","type":"journal-article","created":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T11:42:41Z","timestamp":1776253361000},"source":"Crossref","is-referenced-by-count":0,"title":["Effects of dietary starch concentration and feeding management regimen on bacterial pathogens in liver abscesses and ruminal and colonic epithelial tissues in feedlot steers"],"prefix":"10.1093","volume":"104","author":[{"given":"Kasi N","family":"Schneid","sequence":"first","affiliation":[{"name":"Department of Agricultural Sciences, West Texas A&M University , Canyon, TX 79016,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mina","family":"Abbasi","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Medicine and Pathobiology, Kansas State University , Manhattan, KS 66506,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4085-1158","authenticated-orcid":false,"given":"J Daniel","family":"Young","sequence":"additional","affiliation":[{"name":"Department of Agricultural Sciences, West Texas A&M University , Canyon, TX 79016,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alyssa K","family":"Deters","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Medicine and Pathobiology, Kansas State University , Manhattan, KS 66506,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaorong","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Medicine and Pathobiology, Kansas State University , Manhattan, KS 66506,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leigh Ann","family":"George","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Medicine and Pathobiology, Kansas State University , Manhattan, KS 66506,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0899-878X","authenticated-orcid":false,"given":"T G","family":"Nagaraja","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Medicine and Pathobiology, Kansas State University , Manhattan, KS 66506,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7088-5455","authenticated-orcid":false,"given":"Ty E","family":"Lawrence","sequence":"additional","affiliation":[{"name":"Department of Agricultural Sciences, West Texas A&M University , Canyon, TX 79016,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6632-8557","authenticated-orcid":false,"given":"John T","family":"Richeson","sequence":"additional","affiliation":[{"name":"Department of Agricultural Sciences, West Texas A&M University , Canyon, TX 79016,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7116-0173","authenticated-orcid":false,"given":"Kendall L","family":"Samuelson","sequence":"additional","affiliation":[{"name":"Department of Agricultural Sciences, West Texas A&M University , Canyon, TX 79016,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,5,9]]},"reference":[{"key":"2026062413012154400_skag131-B1","doi-asserted-by":"publisher","first-page":"162","DOI":"10.15232\/aas.2025-02678","article-title":"Culture- and polymerase chain reaction\u2013based prevalence of bacterial pathogens in liver abscesses and ruminal wall tissues of beef-on-dairy feedlot cattle fed finishing diets with no additives, tylosin, or antibiotic alternatives to control liver abscesses","volume":"41","author":"Abbasi","year":"2025","journal-title":"App Anim Sci"},{"key":"2026062413012154400_skag131-B2","doi-asserted-by":"publisher","first-page":"405","DOI":"10.15232\/aas.2025-02704","article-title":"Liver abscess bacterial pathogens in healthy liver tissues, normal tissues of abscessed livers and corresponding ruminal epithelial tissues of beef-on-dairy cattle","volume":"41","author":"Abbasi","year":"2025","journal-title":"App Anim Sci"},{"key":"2026062413012154400_skag131-B3","doi-asserted-by":"publisher","first-page":"3100","DOI":"10.1128\/JCM.01111-15","article-title":"First report of anaerobic isolation of Salmonella enterica from liver abscesses of feedlot cattle","volume":"53","author":"Amachawadi","year":"2015","journal-title":"J Clin Microbiol"},{"key":"2026062413012154400_skag131-B4","doi-asserted-by":"publisher","first-page":"1620","DOI":"10.2527\/jas2015-0261","article-title":"Liver abscesses in cattle: a review of incidence in Holsteins and of bacteriology and vaccine approaches to control in feedlot cattle","volume":"94","author":"Amachawadi","year":"2016","journal-title":"J Anim Sci"},{"key":"2026062413012154400_skag131-B5","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1016\/j.cvfa.2022.08.001","article-title":"Pathogenesis of liver abscesses in cattle","volume":"38","author":"Amachawadi","year":"2022","journal-title":"Vet Clin North Am Food Anim Pract"},{"key":"2026062413012154400_skag131-B6","doi-asserted-by":"publisher","first-page":"3425","DOI":"10.2527\/jas2016.1198","article-title":"Bacterial flora of liver abscesses in crossbred beef cattle and Holstein steers fed finishing diets with or without tylosin","volume":"95","author":"Amachawadi","year":"2017","journal-title":"J Anim Sci"},{"key":"2026062413012154400_skag131-B7","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/j.anaerobe.2005.11.003","article-title":"Human infections with Fusobacterium necrophorum","volume":"12","author":"Brazier","year":"2006","journal-title":"Anaerobe"},{"key":"2026062413012154400_skag131-B8","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1016\/j.cvfa.2022.08.005","article-title":"Roughage and dietary influence on liver abscesses","volume":"38","author":"Bryant","year":"2022","journal-title":"Vet Clin North Am Food Anim Pract"},{"key":"2026062413012154400_skag131-B9","first-page":"270","article-title":"The identification and antimicrobial susceptibility of anaerobic bacteria from pneumonic cattle lungs","volume":"47","author":"Chirino-Trejo","year":"1983","journal-title":"Can J Comp Med"},{"key":"2026062413012154400_skag131-B10","doi-asserted-by":"publisher","first-page":"250","DOI":"10.15232\/aas.2023-02507","article-title":"A real-time PCR assay for the detection and quantification of Fusobacterium necrophorum and Fusobacterium varium in ruminal contents of cattle","volume":"40","author":"Deters","year":"2024","journal-title":"Appl Anim Sci"},{"key":"2026062413012154400_skag131-B11","doi-asserted-by":"publisher","first-page":"244","DOI":"10.15232\/aas.2023-02512","article-title":"First report of isolation of Fusobacterium varium from liver abscesses and ruminal and colonic epithelial tissues of feedlot cattle","volume":"40","author":"Deters","year":"2024","journal-title":"Appl Anim Sci"},{"key":"2026062413012154400_skag131-B12","doi-asserted-by":"publisher","first-page":"635","DOI":"10.15232\/pas.2017-01663","article-title":"Invited review: ruminal microbes, microbial products and systemic inflammation","volume":"33","author":"Garcia","year":"2017","journal-title":"Prof Anim Sci"},{"key":"2026062413012154400_skag131-B13","doi-asserted-by":"publisher","first-page":"358","DOI":"10.15232\/aas.2023-02482","article-title":"Relationship of liver abnormalities with carcass performance and value","volume":"40","author":"Grimes","year":"2024","journal-title":"App Anim Sci"},{"key":"2026062413012154400_skag131-B14","doi-asserted-by":"publisher","first-page":"102","DOI":"10.3389\/fvets.2019.00102","article-title":"Prevalence and serotype diversity of Salmonella in apparently healthy cattle: Systematic review and meta-analysis of published studies, 2000\u20132017","volume":"6","author":"Gutema","year":"2019","journal-title":"Front Vet Sci"},{"key":"2026062413012154400_skag131-B15","doi-asserted-by":"publisher","first-page":"170","DOI":"10.15232\/aas.2021-02228","article-title":"Exploratory observational quantification of liver abscess incidence, specific to region and cattle type, and their associations to viscera value and bacterial flora","volume":"38","author":"Herrick","year":"2022","journal-title":"App Anim Sci"},{"key":"2026062413012154400_skag131-B16","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.anaerobe.2016.09.006","article-title":"The role of Fusobacterium necrophorum in pharyngotonsillitis \u2013 a review","volume":"42","author":"Holm","year":"2016","journal-title":"Anaerobe"},{"key":"2026062413012154400_skag131-B17","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1186\/1297-9716-42-34","article-title":"Animal contact as a source of human non-typhoidal salmonellosis","volume":"42","author":"Hoelzer","year":"2011","journal-title":"Vet Res"},{"key":"2026062413012154400_skag131-B18","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1128\/jcm.32.2.384-387.1994","article-title":"Characterization, distribution, and microbiological associations of Fusobacterium spp. In clinical specimens of animal origin","volume":"32","author":"Jang","year":"1994","journal-title":"J Clin Microbiol"},{"key":"2026062413012154400_skag131-B19","doi-asserted-by":"publisher","first-page":"104623","DOI":"10.1016\/j.livsci.2021.104623","article-title":"Effects of corn stalk inclusion and tylosin on performance, rumination, ruminal papillae morphology, and gut pathogens associated with liver abscesses from finishing beef steers","volume":"251","author":"Jennings","year":"2021","journal-title":"Livest Sci"},{"key":"2026062413012154400_skag131-B20","first-page":"202","article-title":"The rumenitis liver abscess complex in beef cattle","volume":"15","author":"Jensen","year":"1954","journal-title":"Am J Vet Res"},{"key":"2026062413012154400_skag131-B21","doi-asserted-by":"publisher","first-page":"e1-266","DOI":"10.1016\/j.cmi.2014.09.020","article-title":"Fusobacterium necrophorum tonsillitis: an important cause of tonsillitis in adolescents and young adults","volume":"21","author":"Jensen","year":"2015","journal-title":"Clin Microbiol Infect"},{"key":"2026062413012154400_skag131-B22","doi-asserted-by":"publisher","first-page":"e03145-24","DOI":"10.1128\/spectrum.03145-24","article-title":"Characterizing the prevalence of Fusobacterium necrophorum subsp. necrophorum, Fusobacterium necrophorum subsp. funduliforme, and Fusobacterium varium in bovine and ovine semen, bovine gut, and vagino-uterine and fetal microbiota using targeted culturing and qPCR","volume":"13","author":"Kilama","year":"2025","journal-title":"Microbiol Spectr"},{"key":"2026062413012154400_skag131-B23","doi-asserted-by":"publisher","first-page":"3966","DOI":"10.2527\/jas2014-7594","article-title":"Experimental acute rumen acidosis in sheep: consequences on clinical, rumen, and gastrointestinal permeability conditions and blood chemistry","volume":"92","author":"Minuti","year":"2014","journal-title":"J Anim Sci"},{"key":"2026062413012154400_skag131-B24","first-page":"85","volume-title":"Rumen health compendium","author":"Nagaraja","year":"2025","edition":"2nd ed."},{"key":"2026062413012154400_skag131-B25","doi-asserted-by":"publisher","first-page":"287","DOI":"10.2527\/1998.761287x","article-title":"Liver abscesses in feedlot cattle: a review","volume":"76","author":"Nagaraja","year":"1998","journal-title":"J Anim Sci"},{"key":"2026062413012154400_skag131-B26","doi-asserted-by":"publisher","first-page":"E17","DOI":"10.3168\/jds.2006-478","article-title":"Ruminal acidosis in beef cattle: the current microbiological and nutrition outlook","volume":"90","author":"Nagaraja","year":"2007","journal-title":"J Dairy Sci"},{"key":"2026062413012154400_skag131-B27","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1016\/j.anaerobe.2005.01.007","article-title":"Fusobacterium necrophorum infections in animals: pathogenesis and pathogenic mechanisms","volume":"11","author":"Nagaraja","year":"2005","journal-title":"Anaerobe"},{"key":"2026062413012154400_skag131-B28","doi-asserted-by":"crossref","first-page":"4671","DOI":"10.1128\/aem.63.12.4671-4678.1997","article-title":"Ribotyping to compare Fusobacterium necrophorum isolates from bovine liver abscesses, ruminal walls, and ruminal contents","volume":"63","author":"Narayanan","year":"1997","journal-title":"Appl Environ Microbiol"},{"key":"2026062413012154400_skag131-B29","doi-asserted-by":"publisher","first-page":"271","DOI":"10.2460\/ajvr.1998.59.03.271","article-title":"Biochemical and ribotypic comparison of Actinomyces pyogenes and A. pyogenes-like isolates from liver abscesses, ruminal wall, and ruminal contents of cattle","volume":"59","author":"Narayanan","year":"1998","journal-title":"AJVR"},{"key":"2026062413012154400_skag131-B30","doi-asserted-by":"publisher","first-page":"4609","DOI":"10.1128\/IAI.70.8.4609-4620.2002","article-title":"Fusobacterium necrophorum leukotoxin induces activation and apoptosis of bovine leukocytes","volume":"70","author":"Narayanan","year":"2002","journal-title":"Infect Immun"},{"key":"2026062413012154400_skag131-B31","doi-asserted-by":"publisher","first-page":"275","DOI":"10.2527\/1998.761275x","article-title":"Acidosis in cattle: a review","volume":"76","author":"Owens","year":"1998","journal-title":"J Anim Sci"},{"key":"2026062413012154400_skag131-B32","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/j.anaerobe.2019.02.009","article-title":"Leukotoxic activity of Fusobacterium necrophorum of cattle origin","volume":"56","author":"Pillai","year":"2019","journal-title":"Anaerobe"},{"key":"2026062413012154400_skag131-B33","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1186\/s42523-023-00278-0","article-title":"Establishing the link between microbial communities in bovine liver abscesses and the gastrointestinal tract","volume":"5","author":"Pinnell","year":"2023","journal-title":"Anim Microbiome"},{"key":"2026062413012154400_skag131-B34","doi-asserted-by":"publisher","first-page":"2595","DOI":"10.2527\/jas2013-7222","article-title":"Prevalence, severity, and relationship of lung lesions, liver abnormalities, and rumen health scores measured at slaughter of beef cattle","volume":"92","author":"Rezac","year":"2014","journal-title":"J Anim Sci"},{"key":"2026062413012154400_skag131-B35","doi-asserted-by":"publisher","first-page":"2737","DOI":"10.3390\/ijms20112737","article-title":"Pathogenicity and virulence of Trueperella pyogenes: a review","volume":"20","author":"Rzewuska","year":"2019","journal-title":"Int J Mol Sci"},{"key":"2026062413012154400_skag131-B36","doi-asserted-by":"publisher","first-page":"622","DOI":"10.1128\/CMR.00011-07","article-title":"Human infection with Fusobacterium necrophorum (Necrobacillosis) with a focus on Lemierre\u2019s syndrome","volume":"20","author":"Riordan","year":"2007","journal-title":"Clin Microbiol Rev"},{"key":"2026062413012154400_skag131-B37","doi-asserted-by":"publisher","first-page":"e02539\u201325","DOI":"10.1128\/spectrum.02539-25","article-title":"Further delineation of the etiology of liver abscesses in cattle and indication of hindgut as a potential source of pathogens","volume":"13","author":"Salih","year":"2025","journal-title":"Microbiol Spectr."},{"key":"2026062413012154400_skag131-B38","doi-asserted-by":"publisher","first-page":"1817","DOI":"10.3390\/ani10101817","article-title":"Targeting the hindgut to improve health and performance in cattle","volume":"10","author":"Sanz-Fernandez","year":"2020","journal-title":"Animals"},{"key":"2026062413012154400_skag131-B39","first-page":"288","article-title":"Bovine rumenitis\u2013liver abscess complex: a bacteriological review","volume":"73","author":"Scanlan","year":"1983","journal-title":"Cornell Vet"},{"key":"2026062413012154400_skag131-B40","doi-asserted-by":"publisher","first-page":"347","DOI":"10.15232\/aas.2023-02490","article-title":"Effects of dietary composition and feeding management regimen on liver abscess prevalence, growth performance, and carcass outcomes of feedlot steers","volume":"40","author":"Schneid","year":"2024","journal-title":"App Anim Sci"},{"key":"2026062413012154400_skag131-B41","author":"Schneid","year":"2024"},{"key":"2026062413012154400_skag131-B42","author":"Schneid","year":"2025"},{"key":"2026062413012154400_skag131-B43","doi-asserted-by":"publisher","first-page":"616","DOI":"10.7589\/2015-02-039","article-title":"Fusobacterium necrophorum in North American bighorn sheep (Ovis canadensis) pneumonia","volume":"52","author":"Shanthalingam","year":"2016","journal-title":"J Wildl Dis"},{"key":"2026062413012154400_skag131-B44","first-page":"234","article-title":"Ulcerative lesions of the bovine rumen and their possible relation to hepatic abscesses","volume":"5","author":"Smith","year":"1944","journal-title":"Amer J Vet Res"},{"key":"2026062413012154400_skag131-B45","doi-asserted-by":"crossref","first-page":"R1515","DOI":"10.1152\/ajpregu.00120.2010","article-title":"Bovine rumen epithelium undergoes rapid structural adaptations during grain-induced subacute ruminal acidosis","volume":"300","author":"Steele","year":"2011","journal-title":"Amer J Physiol Regul Integr Comp Physiol"},{"key":"2026062413012154400_skag131-B46","doi-asserted-by":"publisher","first-page":"4955","DOI":"10.3168\/jds2015-10351","article-title":"Development and physiology of the rumen and the lower gut: targets for improving gut health","volume":"99","author":"Steele","year":"2016","journal-title":"J Dairy Sci"},{"key":"2026062413012154400_skag131-B47","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.anaerobe.2008.05.005","article-title":"Fusobacterium necrophorum: a ruminal bacterium that invades liver to cause abscesses in cattle","volume":"15","author":"Tadepalli","year":"2009","journal-title":"Anaerobe"},{"key":"2026062413012154400_skag131-B48","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.anaerobe.2007.09.001","article-title":"Leukotoxin operon and differential expressions of the leukotoxin gene in bovine Fusobacterium necrophorum subspecies","volume":"14","author":"Tadepalli","year":"2008","journal-title":"Anaerobe"},{"key":"2026062413012154400_skag131-B49","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1099\/jmm.0.47598-0","article-title":"Human Fusobacterium necrophorum strains have leukotoxin gene and exhibit leukotoxin activity","volume":"57","author":"Tadepalli","year":"2008","journal-title":"J Med Microbiol"},{"key":"2026062413012154400_skag131-B50","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/0378-1135(92)900003-C","article-title":"Factors affecting the leukotoxin activity of Fusobacterium necrophorum","volume":"32","author":"Tan","year":"1992","journal-title":"Vet Microbiol"},{"key":"2026062413012154400_skag131-B51","doi-asserted-by":"publisher","DOI":"10.1093\/jas\/skaf127","article-title":"Economic costs of liver abscesses in U. S. beef feedlot cattle: a comprehensive economic analysis","volume":"103","author":"Taylor","year":"2025","journal-title":"J Anim Sci"},{"key":"2026062413012154400_skag131-B52","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1016\/j.cvfa.2022.07.001","article-title":"Antimicrobial and biological methods to control liver abscesses","volume":"38","author":"Theuer","year":"2022","journal-title":"Vet Clin Food Anim Pract"},{"key":"2026062413012154400_skag131-B53","doi-asserted-by":"publisher","DOI":"10.1093\/jas\/skaf210","article-title":"Evaluation of gastrointestinal and fecal microbial communities as markers of liver abscess risk in beef feedlot cattle","volume":"103","author":"Young","year":"2025","journal-title":"J Anim Sci."},{"key":"2026062413012154400_skag131-B54","doi-asserted-by":"publisher","first-page":"e01746","DOI":"10.1128\/AEM.01746-19","article-title":"SeqSero2: Rapid and improved Salmonella serotype determination using whole-genome sequencing data","volume":"85","author":"Zhang","year":"2019","journal-title":"Appl Environ Microbiol"},{"key":"2026062413012154400_skag131-B55","doi-asserted-by":"publisher","first-page":"1727","DOI":"10.2527\/jas.2006-55","article-title":"Starch digestion by feedlot cattle: predictions from analysis of feed and fecal starch and nitrogen","volume":"85","author":"Zinn","year":"2007","journal-title":"J Anim Sci"}],"container-title":["Journal of Animal Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jas\/advance-article-pdf\/doi\/10.1093\/jas\/skag131\/68261227\/skag131.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jas\/article-pdf\/doi\/10.1093\/jas\/skag131\/68261227\/skag131.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jas\/article-pdf\/doi\/10.1093\/jas\/skag131\/68261227\/skag131.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T17:01:30Z","timestamp":1782320490000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jas\/article\/doi\/10.1093\/jas\/skag131\/8675151"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":55,"URL":"https:\/\/doi.org\/10.1093\/jas\/skag131","relation":{},"ISSN":["0021-8812","1525-3163"],"issn-type":[{"value":"0021-8812","type":"print"},{"value":"1525-3163","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026]]},"published":{"date-parts":[[2026]]},"article-number":"skag131"}}