{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T02:54:58Z","timestamp":1791082498693,"version":"4.1.0"},"reference-count":30,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T00:00:00Z","timestamp":1778284800000},"content-version":"vor","delay-in-days":128,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"ERDF\/EU","award":["PID2023-150622OA-100"],"award-info":[{"award-number":["PID2023-150622OA-100"]}]},{"name":"LiU-Agropor research agreement","award":["86037-293205"],"award-info":[{"award-number":["86037-293205"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The WNT signaling pathway is a central regulator of cell polarity, adhesion, cytoskeletal dynamics, and lineage specification during early embryonic development. Although its roles have been extensively studied in murine and human models, the temporal regulation and pathway architecture of WNT signaling during early porcine development remain poorly defined. Here, we performed a comprehensive transcriptomic analysis to characterize WNT pathway dynamics across key stages of pig in vitro development, including immature oocytes (IMO), mature oocytes (MO), zygotes (ZY), cleaved embryos (2\u20134 cells; CL), and blastocysts (BL). Global analyses revealed major transcriptomic transitions (FDR &amp;lt;0.05; |Fold Change| \u22652) during oocyte maturation and blastocyst formation, whereas zygotes and cleaved embryos exhibited highly similar expression profiles. Module-based and gene-level analyses showed that oocyte maturation is associated with increased expression of extracellular WNT antagonists and components of the \u03b2-catenin destruction complex, together with selective regulation of Frizzled receptors, consistent with tight control of canonical WNT signaling at the MII stage. Following fertilization, this inhibitory configuration was partially relieved, alongside transient upregulation of specific WNT ligands, transcriptional mediators, and adhesion-related components during zygotic genome activation and early cleavage. At the blastocyst stage, WNT signaling became increasingly associated with planar cell polarity and epithelial organization modules. Together, the data reveal a highly dynamic and stage-specific restructuring of WNT signaling during early porcine development. Our findings indicate that precise temporal modulation\u2014rather than uniform activation\u2014of WNT pathway components accompanies the porcine oocyte-to-embryo transition, providing a molecular framework to better understand early developmental regulation and offering insights relevant to reproductive biotechnology and developmental biology.<\/jats:p>","DOI":"10.1093\/jas\/skag134","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T11:30:23Z","timestamp":1776771023000},"source":"Crossref","is-referenced-by-count":1,"title":["Stage-specific remodeling of wingless-related integration sites (WNT) signaling during oocyte-to-embryo transition in pigs"],"prefix":"10.1093","volume":"104","author":[{"given":"Carolina G","family":"Cabrera-Gomez","sequence":"first","affiliation":[{"name":"Department of Biotechnology, National Institute for Agriculture and Food Research and Technology (INIA-CSIC) , Madrid, 28040,","place":["Spain"]},{"name":"Universidad Complutense de Madrid (UCM), Campus de Ciudad Universitaria Faculty of Veterinary Sciences, , Madrid, 28040,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Gascon-Collado","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, National Institute for Agriculture and Food Research and Technology (INIA-CSIC) , Madrid, 28040,","place":["Spain"]},{"name":"Universidad Complutense de Madrid (UCM), Campus de Ciudad Universitaria Faculty of Veterinary Sciences, , Madrid, 28040,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Blanco-Cueto","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, National Institute for Agriculture and Food Research and Technology (INIA-CSIC) , Madrid, 28040,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Belen","family":"Peque\u00f1o","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, National Institute for Agriculture and Food Research and Technology (INIA-CSIC) , Madrid, 28040,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heriberto","family":"Rodriguez-Martinez","sequence":"additional","affiliation":[{"name":"Department of Biomedical & Clinical Sciences (BKV), BKH\/Obstetrics & Gynecology, Faculty of Medicine and Health Sciences, Link\u00f6ping University , Link\u00f6ping, SE-58185,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6811-0191","authenticated-orcid":false,"given":"Cristina A","family":"Martinez-Serrano","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, National Institute for Agriculture and Food Research and Technology (INIA-CSIC) , Madrid, 28040,","place":["Spain"]},{"name":"Department of Biomedical & Clinical Sciences (BKV), BKH\/Obstetrics & Gynecology, Faculty of Medicine and Health Sciences, Link\u00f6ping University , Link\u00f6ping, SE-58185,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,5,9]]},"reference":[{"key":"2026051803413680400_skag134-B1","doi-asserted-by":"publisher","first-page":"1324647","DOI":"10.3389\/FVETS.2023.1324647\/BIBTEX","article-title":"Transient suppression of Wnt signaling in poor-quality buffalo oocytes improves their developmental competence","volume":"10","author":"Ahuja","year":"2023","journal-title":"Front Vet Sci"},{"key":"2026051803413680400_skag134-B3","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1016\/j.diff.2013.11.005","article-title":"Pig blastocyst-uterine 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