{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:35:10Z","timestamp":1790670910432,"version":"4.1.0"},"reference-count":51,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T00:00:00Z","timestamp":1779408000000},"content-version":"vor","delay-in-days":141,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Regional Development Fund of the European Union and Greek national funds"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The inclusion of microalgae in ruminant nutrition offers a well-documented approach to enhance the n-3 polyunsaturated fatty acid (PUFA) profile of animal products while potentially modulating immune and oxidative functions. This study evaluated the long-term effects of dietary supplementation with Schizochytrium spp. and Nannochloropsis gaditana on the oxidative-inflammatory interplay in dairy ewes. Thirty-two crossbred dairy ewes in early lactation (50\u2009\u00b1\u20095\u2009d in milk) were divided into four homogeneous groups (n\u2009=\u20098 per group) based on milk yield, body weight, and age. The control group (CON) received a concentrate without microalgae supplementation, whereas the treated groups received concentrates supplemented daily with 30\u2009g of Schizochytrium spp. (SC30) or with 30\u2009g (MB30) or 40\u2009g (MB40) of a microalgae blend of Schizochytrium spp. and N. gaditana in a 70:30 ratio. Accordingly, MB30 supplied 21\u2009g of Schizochytrium spp. and 9\u2009g of N. gaditana, while MB40 supplied 28\u2009g of Schizochytrium spp. and 12\u2009g of N. gaditana. On the 30th, 60th, and 90th day of the experiment, blood monocytes and neutrophils were isolated to assess the expression of genes involved in antioxidant defense, eicosanoid metabolism, and immune response. Microalgae supplementation did not affect feed intake but altered the dietary fatty acid profile, and increased the intake of long-chain PUFA (DHA, docosahexaenoic acid; n-6 DPA, docosapentaenoic acid; EPA, eicosapentaenoic acid). Moderate supplementation of microalgae blend (MB30) upregulated GPX1 and GPX2 expression, while higher inclusion (MB40) enhanced CAT transcription but suppressed SOD2 and GPX2 in monocytes. Genes associated with eicosanoid synthesis (PLA2G4A, COX, LTA4H, ALOX5AP) were significantly downregulated in monocytes, particularly in MB40. Likewise, key immune-related transcripts (TLR4, IFNG, IL1B, IL2) were markedly decreased in MB40. Correlation analysis revealed that long-chain PUFA were inversely associated with pro-inflammatory and oxidative genes. Overall, the combined supplementation of Schizochytrium spp. and N. gaditana modulated redox-sensitive and immune signaling pathways, suggesting a transcriptional profile consistent with reduced expression of pro-inflammatory genes in ewes.<\/jats:p>","DOI":"10.1093\/jas\/skag165","type":"journal-article","created":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T11:38:52Z","timestamp":1779190732000},"source":"Crossref","is-referenced-by-count":1,"title":["Dietary microalgae\n                    <i>Schizochytrium<\/i>\n                    spp. and\n                    <i>Nannochloropsis gaditana<\/i>\n                    modulate antioxidant and immune-related gene expression in ewes\u2019 monocytes and neutrophils"],"prefix":"10.1093","volume":"104","author":[{"given":"Alexandros","family":"Mavrommatis","sequence":"first","affiliation":[{"name":"Laboratory of Nutritional Physiology and Feeding, Department of Animal Science, School of Animal Biosciences, Agricultural University of 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