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Because 5-HTP bypasses the rate-limiting step of serotonin synthesis, it has been used to experimentally increase circulating serotonin in ruminants via enteral or intravenous administration; however, the capability of ruminally supplied 5-HTP to escape microbial degradation and influence peripheral serotonin metabolism and vascular function has not been fully characterized. The objective of this study was to evaluate abomasal and ruminal 5-HTP administration on peripheral serotonin metabolism and caudal artery hemodynamics in growing beef steers. Five ruminally cannulated Angus \u00d7 Holstein steers (body weight [BW] = 284\u2009\u00b1\u200910.6\u2009kg) were randomly assigned to a 5\u2009\u00d7\u20095 Latin square design. Treatments consisted of water (negative control), abomasal infusion of 0.5\u2009mg 5-HTP\/kg BW (positive control), or ruminal infusions of 1, 2, or 3\u2009mg 5-HTP\/kg BW administered as daily pulse doses for 5 d, with 5-d washout periods. Blood samples were collected serially over 24\u2009h on d 1 and 5 of each period for analysis of tryptophan, kynurenine, 5-HTP, serotonin, and 5-hydroxyindolacetic acid. Caudal artery hemodynamics were assessed using color Doppler ultrasonography. Dry matter intake and plasma biomarkers of energy and protein metabolism were not affected (P\u2009\u2265\u20090.15) by enteral 5-HTP treatments. Abomasal 5-HTP infusion rapidly increased (P\u2009&amp;lt;\u20090.05) serum 5-HTP and increased serum serotonin concentration on d 5. Increasing ruminal 5-HTP infusion increased serum serotonin concentration in a dose-dependent manner, indicating partial escape from ruminal degradation and effective post-ruminal utilization. Relative to baseline, ruminal infusion of 3\u2009mg 5-HTP\/kg BW increased caudal artery cross-sectional area, demonstrating peripheral vasodilation (P\u2009&amp;lt;\u20090.05), whereas lower ruminal 5-HTP doses and abomasal 5-HTP infusion did not elicit measurable vascular responses. These results demonstrate that ruminal supplementation of 5-HTP can increase circulating serotonin and, at sufficient doses, promote peripheral vasodilation in growing beef steers. This study provides the first in vivo evidence of arterial vasodilation associated with 5-HTP supplementation in cattle and establishes ruminal delivery as a viable route for modulating serotonergic and vascular function.<\/jats:p>","DOI":"10.1093\/jas\/skag204","type":"journal-article","created":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T11:36:50Z","timestamp":1782992210000},"source":"Crossref","is-referenced-by-count":0,"title":["Ruminal 5-hydroxytryptophan increases serum serotonin and peripheral vasodilation in growing beef cattle"],"prefix":"10.1093","volume":"104","author":[{"given":"Ellem M A","family":"Matos","sequence":"first","affiliation":[{"name":"Department of Animal and Food Sciences, University of Kentucky , Lexington, KY 40546,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5187-1920","authenticated-orcid":false,"given":"David 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