{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T04:55:38Z","timestamp":1790571338653,"version":"4.1.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:00:00Z","timestamp":1790380800000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Mammalian spermatogenesis is highly sensitive to temperature fluctuations. Sertoli cells provide essential support for germ cell development and are vulnerable to heat stress-induced cellular damage. Heat stress, known to impair spermatogenesis and induce cellular senescence in various cells, poses a significant threat to male fertility. Given that leptin possesses anti-senescence potential, this study investigates its ability to alleviate heat stress-induced senescence in Sertoli cells and explores the underlying molecular mechanisms. Our results demonstrate that heat stress triggers accumulation of reactive oxygen species (ROS) (P &amp;lt; 0.05), resulting in DNA damage that ultimately drives Sertoli cell senescence. Leptin effectively mitigates senescence through multiple pathways. Specifically, leptin enhances antioxidant enzyme activity (P &amp;lt; 0.05) and inhibits the ATM-p53 pathway, thereby reducing DNA damage. Furthermore, leptin activates the extracellular signal-regulated kinase 1\/2 (ERK1\/2) pathway, reversing G1 phase arrest. Additionally, leptin promotes autophagy via the AMPK-mTORC1 pathway. Concurrently, leptin reduces the activity of senescence-associated \u03b2-galactosidase (SA-\u03b2-gal) and lessens the release of senescence-associated secretory phenotype (SASP) factors (P &amp;lt; 0.05), thus slowing senescence progression. In conclusion, leptin exerts a protective effect against heat stress-induced senescence in Sertoli cells, offering novel insights into potential strategies for the prevention of heat stress-related male infertility.<\/jats:p>","DOI":"10.1093\/jas\/skag314","type":"journal-article","created":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T12:18:27Z","timestamp":1790425107000},"source":"Crossref","is-referenced-by-count":0,"title":["Leptin mitigates heat stress-induced senescence in porcine Sertoli cells through modulation of ATM-p53, ERK1\/2, and AMPK-mTORC1 signaling"],"prefix":"10.1093","author":[{"given":"Ming","family":"Guo","sequence":"first","affiliation":[{"name":"Northwest A&F University College of Animal Science and Technology, , Yangling, Shaanxi 712100,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yawen","family":"Gao","sequence":"additional","affiliation":[{"name":"Shaanxi University of Technology School of Biological Science and Engineering, , Hanzhong, Shaanxi 723001,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shaanxi University of Technology School of Biological Science and Engineering, , Hanzhong, Shaanxi 723001,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhendong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Qingdao Agricultural University College of Animal Science and Technology, , Qingdao 266109,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Lin","sequence":"additional","affiliation":[{"name":"Sichuan Agricultural University Animal Nutrition Institute, , Chengdu, 611130,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongzhao","family":"Lu","sequence":"additional","affiliation":[{"name":"Shaanxi University of Technology School of Biological Science and Engineering, , Hanzhong, Shaanxi 723001,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Cheng","sequence":"additional","affiliation":[{"name":"Shaanxi University of Technology School of Biological Science and Engineering, , Hanzhong, Shaanxi 723001,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shaanxi University of Technology School of Biological Science and Engineering, , Hanzhong, Shaanxi 723001,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Luo","sequence":"additional","affiliation":[{"name":"Northwest A&F University College of Animal Science and Technology, , Yangling, Shaanxi 712100,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxian","family":"Zeng","sequence":"additional","affiliation":[{"name":"Northwest A&F University College of Animal Science and Technology, , Yangling, Shaanxi 712100,","place":["China"]},{"name":"Shaanxi University of Technology School of Biological Science and Engineering, , Hanzhong, Shaanxi 723001,","place":["China"]},{"name":"Qinba State Key Laboratory of Biological Resources and Ecological Environment (Incubation) , Hanzhong, Shaanxi 723001,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,26]]},"container-title":["Journal of Animal Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jas\/advance-article-pdf\/doi\/10.1093\/jas\/skag314\/71409165\/skag314.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jas\/advance-article-pdf\/doi\/10.1093\/jas\/skag314\/71409165\/skag314.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T04:14:02Z","timestamp":1790568842000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jas\/advance-article\/doi\/10.1093\/jas\/skag314\/8837393"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,26]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/jas\/skag314","relation":{},"ISSN":["0021-8812","1525-3163"],"issn-type":[{"value":"0021-8812","type":"print"},{"value":"1525-3163","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,26]]},"article-number":"skag314"}}