{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T16:56:14Z","timestamp":1791564974579,"version":"4.3.4"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The aim of this study was to establish suitable conditions for the transfer of cloned \u1ec8 pig embryos by determining the appropriate embryonic developmental stage, number of SCNT \u1ec8 pig embryos transferred, and synchronization method. Under the conditions of the present study, our results demonstrated that the transfer of SCNT-derived blastocysts resulted in a significantly higher pregnancy rate than the transfer of embryos at 20\u2009h post-SCNT (P\u2009&amp;lt;\u20090.05). The transfer of 60 SCNT-derived blastocysts resulted in the lowest pregnancy rate (P\u2009&amp;lt;\u20090.05). Although variation in the number of blastocysts transferred (80, 100, 120, or 150) did not significantly affect pregnancy outcomes, none of the recipients receiving 80 SCNT-derived blastocysts subsequently farrowed. Increasing the number of SCNT-derived blastocysts transferred from 100 to 150 did not significantly affect the proportion of recipients that successfully farrowed. Estrus synchronization of recipients with PGF2\u03b1, followed by eCG and hCG administration, improved the efficiency of cloned pig embryo transfer. The pregnancy rate in recipients synchronized with PGF2\u03b1 + eCG + hCG was significantly higher than that in the eCG + hCG group (P\u2009&amp;lt;\u20090.05). Under the conditions of the present study, synchronization with PGF2\u03b1, eCG, and hCG combined with the transfer of at least 100 SCNT-derived blastocysts appeared to provide a more favorable strategy for improving the efficiency of cloned \u1ec8 pig embryo transfer.<\/jats:p>","DOI":"10.1093\/jas\/skag330","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T11:45:41Z","timestamp":1791546341000},"source":"Crossref","is-referenced-by-count":0,"title":["Optimization of embryonic developmental stage, embryo transfer number, synchronization method for enhancing the efficiency of cloned \u1ec8 pig embryo transfer"],"prefix":"10.1093","author":[{"given":"Au Thi","family":"Hoang","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Cell Technology, Vietnam Institute of Animal and Veterinary Sciences , Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vu Thi Thu","family":"Huong","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Cell Technology, Vietnam Institute of Animal and Veterinary Sciences , Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huong Le Thi","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Cell Technology, Vietnam Institute of Animal and Veterinary Sciences , Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huu Xuan","family":"Quan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Cell Technology, Vietnam Institute of Animal and Veterinary Sciences , Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hieu Trung","family":"Phan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Cell Technology, Vietnam Institute of Animal and Veterinary Sciences , Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dat","family":"Van Le","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Cell Technology, Vietnam Institute of Animal and Veterinary Sciences , Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huong Thi","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Cell Technology, Vietnam Institute of Animal and Veterinary Sciences , Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duc Viet","family":"Phan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Cell Technology, Vietnam Institute of Animal and Veterinary Sciences , Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nhung Tuyet Thi","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Cell Technology, Vietnam Institute of Animal and Veterinary Sciences , Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anh Lan Thi","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Cell Technology, Vietnam Institute of Animal and Veterinary Sciences , Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yen Kim Thi","family":"Pham","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Cell Technology, Vietnam Institute of Animal and Veterinary Sciences , Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan Doan","family":"Pham","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Cell Technology, Vietnam Institute of Animal and Veterinary Sciences , Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Thi","family":"Nhien","sequence":"additional","affiliation":[{"name":"Vietnam National University of Agriculture Faculty of Biotechnology, , Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen","family":"Thi Van Anh","sequence":"additional","affiliation":[{"name":"Phenikaa University Faculty of Biomedical Science, , Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Hai","family":"Yen","sequence":"additional","affiliation":[{"name":"Ministry of Agriculture and Environment Department of Science and Technology, , Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Van Khanh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Cell Technology, Vietnam Institute of Animal and Veterinary Sciences , Hanoi, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,9]]},"container-title":["Journal of Animal 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