{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:30:06Z","timestamp":1770748206935,"version":"3.49.0"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2018,10,12]],"date-time":"2018-10-12T00:00:00Z","timestamp":1539302400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,12,3]]},"DOI":"10.1093\/jas\/sky371","type":"journal-article","created":{"date-parts":[[2018,10,12]],"date-time":"2018-10-12T18:14:14Z","timestamp":1539368054000},"page":"5010-5023","source":"Crossref","is-referenced-by-count":13,"title":["Effect of induced hypocalcemia in nonlactating, nonpregnant Holstein cows fed negative DCAD with low, medium, or high concentrations of calcium"],"prefix":"10.1093","volume":"96","author":[{"given":"Laura A","family":"Amundson","sequence":"first","affiliation":[{"name":"Department of Dairy Science, University of Wisconsin-Madison, Madison"},{"name":"Department of Animal Science, University of Wisconsin-Madison, Madison"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela D","family":"Rowson","sequence":"additional","affiliation":[{"name":"Phibro Animal Health Corporation, Teaneck, NJ"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter M","family":"Crump","sequence":"additional","affiliation":[{"name":"Department of Dairy Science, University of Wisconsin-Madison, Madison"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Austin P","family":"Prichard","sequence":"additional","affiliation":[{"name":"Department of Dairy Science, University of Wisconsin-Madison, Madison"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrienne A","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Dairy Science, University of Wisconsin-Madison, Madison"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Collin E","family":"Wimmler","sequence":"additional","affiliation":[{"name":"Department of Dairy Science, University of Wisconsin-Madison, Madison"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marisa","family":"Klister","sequence":"additional","affiliation":[{"name":"Department of Dairy Science, University of Wisconsin-Madison, Madison"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samantha R","family":"Weaver","sequence":"additional","affiliation":[{"name":"Department of Dairy Science, University of Wisconsin-Madison, Madison"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott S","family":"Bascom","sequence":"additional","affiliation":[{"name":"Phibro Animal Health Corporation, Teaneck, NJ"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis E","family":"Nuzback","sequence":"additional","affiliation":[{"name":"Phibro Animal Health Corporation, Teaneck, NJ"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken P","family":"Zanzalari","sequence":"additional","affiliation":[{"name":"Phibro Animal Health Corporation, Teaneck, NJ"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura L","family":"Hernandez","sequence":"additional","affiliation":[{"name":"Department of Dairy Science, University of Wisconsin-Madison, Madison"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2018,10,12]]},"reference":[{"key":"key\n\t\t\t\t2018120309335932900_CIT0001","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1016\/j.cca.2011.01.004","article-title":"Ionized calcium","volume":"412","author":"Baird","year":"2011","journal-title":"Clin. Chim. Acta"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0002","doi-asserted-by":"crossref","first-page":"850","DOI":"10.3168\/jds.S0022-0302(81)82657-4","article-title":"Concentration of calcium, phosphorus, and 1,25-dihydroxyvitamin D in plasma of dairy cows during the lactation cycle","volume":"64","author":"Barton","year":"1981","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0003","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.3168\/jds.S0022-0302(76)84325-1","article-title":"Effects of a low calcium diet on feed intake, milk production, and response to blood calcium challenge in lactating Holstein cows","volume":"59","author":"Belyea","year":"1976","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0004","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1017\/S0021859600040491","article-title":"Studies of the skeleton of the sheep: the effect of different levels of dietary calcium during pregnancy and lactation on individual bones","volume":"46","author":"Benzie","year":"1955","journal-title":"J. Agri. Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0005","doi-asserted-by":"crossref","first-page":"2939","DOI":"10.3168\/jds.S0022-0302(84)81657-4","article-title":"Manipulating dietary anions and cations for prepartum dairy cows to reduce incidence of milk fever","volume":"67","author":"Block","year":"1984","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0006","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1080\/01652176.1986.9694013","article-title":"Effects of low and high calcium intake prepartum on calcium mobilization rate around parturition in dairy cows","volume":"8","author":"van de Braak","year":"1986","journal-title":"Vet. Q"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0007","doi-asserted-by":"crossref","first-page":"704","DOI":"10.3168\/jds.S0022-0302(06)72133-6","article-title":"Effect of prepartum dietary calcium on intake and serum and urinary mineral concentrations of cows","volume":"89","author":"Chan","year":"2006","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0009","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1006\/jtbi.2001.2422","article-title":"Calcium homeostasis and parturient hypocalcemia: an integral feedback perspective","volume":"214","author":"El-Samad","year":"2002","journal-title":"J. Theor. Biol"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0010","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.cvfa.2004.06.003","article-title":"Macromineral disorders of the transition cow","volume":"20","author":"Goff","year":"2004","journal-title":"Vet. Clin. North Am. Food Anim. Pract"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0011","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.tvjl.2007.12.020","article-title":"The monitoring, prevention, and treatment of milk fever and subclinical hypocalcemia in dairy cows","volume":"176","author":"Goff","year":"2008","journal-title":"Vet. J"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0012","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.cvfa.2014.04.003","article-title":"Calcium and magnesium disorders","volume":"30","author":"Goff","year":"2014","journal-title":"Vet. Clin. North Am. Food Anim. Pract"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0013","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.3168\/jds.S0022-0302(97)76055-7","article-title":"Physiological changes at parturition and their relationship to metabolic disorders","volume":"80","author":"Goff","year":"1997","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0014","doi-asserted-by":"crossref","first-page":"1520","DOI":"10.3168\/jds.2013-7467","article-title":"Diet-induced pseudohypoparathyroidism: a hypocalcemia and milk fever risk factor","volume":"97","author":"Goff","year":"2014","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0015","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.3168\/jds.S0022-0302(74)85034-4","article-title":"Prevention of parturient paresis by a prepartum, calcium-deficient diet","volume":"57","author":"Goings","year":"1974","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0016","doi-asserted-by":"crossref","first-page":"217","DOI":"10.3168\/jds.S0022-0302(81)82557-X","article-title":"Vitamin D metabolites in plasma of cows fed a prepartum low-calcium diet for prevention of parturient hypocalcemia","volume":"64","author":"Green","year":"1981","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0017","doi-asserted-by":"crossref","first-page":"727","DOI":"10.3168\/jds.2010-3230","article-title":"Periparturient effects of feeding a low dietary cation-anion difference diet on acid-base, calcium, and phosphorus homeostasis and on intravenous glucose tolerance test in high-producing dairy cows","volume":"94","author":"Grunberg","year":"2011","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0018","doi-asserted-by":"crossref","first-page":"5050","DOI":"10.3168\/jds.2016-12441","article-title":"Endocrine and metabolic changes in transition dairy cows are affected by prepartum infusions of a serotonin precursor","volume":"100","author":"Hernandez-Castellano","year":"2017","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0019","doi-asserted-by":"crossref","first-page":"238","DOI":"10.3168\/jds.2008-1357","article-title":"Timothy hays differing in dietary cation-anion difference affect the capability of dairy cows to maintain their calcium homeostasis","volume":"92","author":"Heron","year":"2009","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0021","doi-asserted-by":"crossref","first-page":"933","DOI":"10.3168\/jds.S0022-0302(74)84989-1","article-title":"Combating milk fever","volume":"57","author":"Jorgensen","year":"1974","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0022","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1093\/jn\/112.3.480","article-title":"Relationships between prepartal dietary calcium and phosphorus, vitamin D metabolism, and parturient paresis in dairy cows","volume":"112","author":"Kichura","year":"1982","journal-title":"J. Nutr"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0023","doi-asserted-by":"crossref","first-page":"e110190","DOI":"10.1371\/journal.pone.0110190","article-title":"Peripheral serotonin regulates maternal calcium trafficking in mammary epithelial cells during lactation in mice","volume":"9","author":"Laporta","year":"2014","journal-title":"PLoS One"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0024","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1530\/JOE-14-0693","article-title":"Increasing serotonin concentrations alter calcium and energy metabolism in dairy cows","volume":"226","author":"Laporta","year":"2015","journal-title":"J. Endocrinol"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0025","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.domaniend.2013.01.005","article-title":"Feeding 5-hydroxy-l-tryptophan during the transition from pregnancy to lactation increases calcium mobilization from bone in rats","volume":"44","author":"Laporta","year":"2013","journal-title":"Domest. Anim. Endocrinol"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0026","doi-asserted-by":"crossref","first-page":"669","DOI":"10.3168\/jds.S0022-0302(06)72130-0","article-title":"Hypocalcemia in dairy cows: meta-analysis and dietary cation anion difference theory revisited","volume":"89","author":"Lean","year":"2006","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0027","doi-asserted-by":"crossref","first-page":"9285","DOI":"10.3168\/jds.2017-12971","article-title":"Short communication: relationship between methods for measurement of serum electrolytes and the relationship between ionized and total calcium and neutrophil oxidative burst activity in early postpartum dairy cows","volume":"100","author":"Leno","year":"2017","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0028","doi-asserted-by":"crossref","first-page":"4604","DOI":"10.3168\/jds.2016-12221","article-title":"Effects of prepartum dietary cation-anion difference on aspects of peripartum mineral and energy metabolism and performance of multiparous Holstein cows","volume":"100","author":"Leno","year":"2017","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0029","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1111\/j.1439-0396.2006.00651.x","article-title":"Influence of different calcium contents in diets supplemented with anionic salts on bone metabolism in periparturient dairy cows","volume":"91","author":"Liesegang","year":"2007","journal-title":"J. Anim. Physiol. Anim. Nutr. (Berl)"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0030","doi-asserted-by":"crossref","first-page":"135","DOI":"10.3181\/00379727-129-33269","article-title":"Insulin: evidence for inhibition of release in spontaneous hypocalcemia","volume":"129","author":"Littledike","year":"1968","journal-title":"Proc. Soc. Exp. Biol. Med"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0031","doi-asserted-by":"crossref","first-page":"7158","DOI":"10.3168\/jds.2012-5812","article-title":"Evaluation of peripartal calcium status, energetic profile, and neutrophil function in dairy cows at low or high risk of developing uterine disease","volume":"95","author":"Martinez","year":"2012","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0032","doi-asserted-by":"crossref","first-page":"874","DOI":"10.3168\/jds.2013-7408","article-title":"Effect of induced subclinical hypocalcemia on physiological responses and neutrophil function in dairy cows","volume":"97","author":"Martinez","year":"2014","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0033","first-page":"3754","article-title":"Patterns of circulating serotonin and related metabolites in multiparous dairy cows in the peripartum period","volume":"98","author":"Moore","year":"2015","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0034","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.3168\/jds.S0022-0302(00)75091-0","article-title":"Effects of altering dietary cation-anion difference on calcium and energy metabolism in peripartum cows","volume":"83","author":"Moore","year":"2000","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0035","doi-asserted-by":"crossref","first-page":"1613","DOI":"10.3168\/jds.S0022-0302(72)85729-1","article-title":"Effect of estrogen and progesterone on feed intake and hydroxyproline excretion following induced hypocalcemia in dairy cows","volume":"55","author":"Muir","year":"1972","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0036","doi-asserted-by":"crossref","first-page":"3796","DOI":"10.3168\/jds.2016-11970","article-title":"Risk factors associated with postpartum subclinical hypocalcemia in dairy cows","volume":"100","author":"Neves","year":"2017","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0037","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.3168\/jds.2017-13779","article-title":"Method comparison and validation of a prototype device for measurement of ionized calcium concentrations cow-side against a point-of-care instrument and a benchtop blood-gas analyzer reference method","volume":"101","author":"Neves","year":"2018","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0038","volume-title":"Nutrient requirements of dairy cattle","author":"NRC","year":"2001","edition":"7"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0039","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.anifeedsci.2011.01.002","article-title":"Dietary Ca concentration to minimize the risk of hypocalcemia in dairy cows affected by the dietary cation-anion differences","volume":"164","author":"Oba","year":"2011","journal-title":"Anim. Feed Sci. Technol"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0040","doi-asserted-by":"crossref","first-page":"3900","DOI":"10.3168\/jds.S0022-0302(91)78583-4","article-title":"Meta-analysis of nutritional risk factors for milk fever in dairy cattle","volume":"74","author":"Oetzel","year":"1991","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0041","doi-asserted-by":"crossref","first-page":"3302","DOI":"10.3168\/jds.S0022-0302(88)79935-X","article-title":"Ammonium chloride and ammonium sulfate for prevention of parturient paresis in dairy cows","volume":"71","author":"Oetzel","year":"1988","journal-title":"J. Dairy Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0043","author":"Robinson","year":"2010"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0044","doi-asserted-by":"crossref","first-page":"1330","DOI":"10.2460\/ajvr.2005.66.1330","article-title":"Prediction of serum ionized calcium concentration by use of serum total calcium concentration in dogs","volume":"66","author":"Schenck","year":"2005","journal-title":"Am. J. Vet. Res"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0045","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/j.cvsm.2008.01.010","article-title":"Calcium: total or ionized","volume":"38","author":"Schenck","year":"2008","journal-title":"Vet. Clin. North Am. Small Anim. Pract"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0046","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1751-0147-43-1","article-title":"Milk fever control principles: a review","volume":"43","author":"Thilsing-Hansen","year":"2002","journal-title":"Acta Vet. Scand"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0047","doi-asserted-by":"crossref","first-page":"217","DOI":"10.3109\/00365518909089086","article-title":"Comparison of serum total calcium, albumin-corrected total calcium, and ionized calcium in 1213 patients with suspected calcium disorders","volume":"49","author":"Thode","year":"1989","journal-title":"Scand. J. Clin. Lab. Invest"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0048","doi-asserted-by":"crossref","first-page":"1238","DOI":"10.2527\/1992.7041238x","article-title":"Influence of dietary cation-anion balance during the dry period on the occurrence of parturient paresis in cows fed excess calcium","volume":"70","author":"Tucker","year":"1992","journal-title":"J. Anim. Sci"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0049","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1530\/JOE-16-0038","article-title":"Elevation of circulating serotonin improves calcium dynamics in the peripartum dairy cow","volume":"230","author":"Weaver","year":"2016","journal-title":"J. Endocrinol"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0051","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1210\/endo-93-4-761","article-title":"Suppression of insulin secretion during induced hypocalcemia","volume":"93","author":"Witzel","year":"1973","journal-title":"Endocrinology"},{"key":"key\n\t\t\t\t2018120309335932900_CIT0052","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1111\/j.1749-6632.2009.05249.x","article-title":"Interactions between breast, bone, and brain regulate mineral and skeletal metabolism during lactation","volume":"1192","author":"Wysolmerski","year":"2010","journal-title":"Ann. N. Y. Acad. Sci"}],"container-title":["Journal of Animal Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jas\/article-pdf\/96\/12\/5010\/26982493\/sky371.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,26]],"date-time":"2019-10-26T15:05:10Z","timestamp":1572102310000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jas\/article\/96\/12\/5010\/5128796"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,12]]},"references-count":48,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2018,10,12]]},"published-print":{"date-parts":[[2018,12,3]]}},"URL":"https:\/\/doi.org\/10.1093\/jas\/sky371","relation":{},"ISSN":["0021-8812","1525-3163"],"issn-type":[{"value":"0021-8812","type":"print"},{"value":"1525-3163","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2018,12]]},"published":{"date-parts":[[2018,10,12]]}}}