{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:24:28Z","timestamp":1787009068369,"version":"build-2736575974"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2020,2,12]],"date-time":"2020-02-12T00:00:00Z","timestamp":1581465600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Plan Nacional Sobre Drogas"},{"DOI":"10.13039\/501100003751","name":"Ministerio de Sanidad, Servicios Sociales e Igualdad","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003751","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Gobierno de Espa\u00f1a","award":["2016I005"],"award-info":[{"award-number":["2016I005"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,7,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>An LC\u2013MS-MS method for the determination of 14 benzodiazepines (BZDs) (alprazolam, \u03b1-hydroxyalprazolam, clonazepam, bromazepam, diazepam, nordiazepam, lorazepam, lormetazepam, oxazepam, flunitrazepam, 7-aminoflunitrazepam, triazolam, midazolam and zolpidem) and 15 antidepressants (ADs) (amitriptyline, nortriptyline, imipramine, desipramine, clomipramine, norclomipramine, fluoxetine, norfluoxetine, sertraline, norsertraline, paroxetine, venlafaxine, desmethylvenlafaxine, citalopram and desmethylcitalopram) in meconium was developed and validated. Meconium samples (0.25\u00a0\u00b1\u00a00.02\u00a0g) were homogenized in methanol and subjected to mixed-mode cation exchange solid-phase extraction. Chromatographic separation was performed in reversed phase, with a gradient of 0.1% formic acid in 2\u00a0mM ammonium formate and acetonitrile. Two different chromatographic gradient methods were employed, one for the separation of ADs and another for BZDs. Analytes were monitored by tandem mass spectrometry employing electrospray positive mode in MRM mode (2 transitions per compound). Method validation included: linearity [n\u00a0=\u00a05, limit of quantification (LOQ) to 400\u00a0ng\/g], limits of detection (n\u00a0=\u00a06, 1\u201320\u00a0ng\/g), LOQ (n\u00a0=\u00a09, 5\u201320\u00a0ng\/g), selectivity (no endogenous or exogenous interferences), accuracy (n\u00a0=\u00a015, 90.6\u2013111.5%), imprecision (n\u00a0=\u00a015, 0\u201314.6%), matrix effect (n\u00a0=\u00a010, \u221273 to 194.9%), extraction efficiency (n\u00a0=\u00a06, 35.9\u201391.2%), process efficiency (n\u00a0=\u00a06, 20.1\u2013188.2%), stability 72\u00a0h in the autosampler (n\u00a0=\u00a03, \u22128.5 to 9%) and freeze\/thaw stability (n\u00a0=\u00a03, \u22121.2 to \u221247%). The method was applied to four meconium specimens, which were analyzed with and without hydrolysis (enzymatic and alkaline). The authentic meconium samples tested positive for alprazolam, \u03b1-hydroxyalprazolam, clonazepam, diazepam, nordiazepam, fluoxetine, norfluoxetine, clomipramine and norclomipramine. Therefore, the present LC\u2013MS-MS method allows a high throughput determination of the most common BZDs and ADs in meconium, which could be useful in clinical and forensic settings.<\/jats:p>","DOI":"10.1093\/jat\/bkaa012","type":"journal-article","created":{"date-parts":[[2020,2,6]],"date-time":"2020-02-06T15:09:30Z","timestamp":1581001770000},"page":"580-588","source":"Crossref","is-referenced-by-count":21,"title":["LC\u2013MS-MS Method for the Determination of Antidepressants and Benzodiazepines in Meconium"],"prefix":"10.1093","volume":"44","author":[{"given":"A","family":"L\u00f3pez-Rabu\u00f1al","sequence":"first","affiliation":[{"name":"Servizo de Toxicolox\u00eda, Instituto de Ciencias Forenses, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Lendoiro","sequence":"additional","affiliation":[{"name":"Servizo de Toxicolox\u00eda, Instituto de Ciencias Forenses, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Concheiro","sequence":"additional","affiliation":[{"name":"Department of Sciences, John Jay College of Criminal Justice, City University of New York, 10019 New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"L\u00f3pez-Rivadulla","sequence":"additional","affiliation":[{"name":"Servizo de Toxicolox\u00eda, Instituto de Ciencias Forenses, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Cruz","sequence":"additional","affiliation":[{"name":"Servizo de Toxicolox\u00eda, Instituto de Ciencias Forenses, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, 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