{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T16:34:51Z","timestamp":1793896491581,"version":"4.8.0"},"reference-count":17,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2020,8,5]],"date-time":"2020-08-05T00:00:00Z","timestamp":1596585600000},"content-version":"vor","delay-in-days":34,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Department of Clinical Pharmacology"},{"name":"Clinic of Laboratory Medicine"},{"name":"St. Olavs University Hospital"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,4,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Due to its specificity, phosphatidylethanol (PEth) 16:0\/18:1 has gained increased popularity as a marker for high alcohol consumption in recent years. As conflicting results regarding the stability of PEth 16:0\/18:1 in whole blood have been published, there are still uncertainties related to optimum handling, transport and storage of blood samples for the analysis of PEth 16:0\/18:1.<\/jats:p>\n               <jats:p>A stability study where whole blood samples were drawn from healthy volunteers, who had ingested alcohol, is presented. The samples were collected in tubes with ethylenediamine tetraacetic acid (EDTA) and heparin as additives and stored under standardized conditions within 1\u00a0h of blood sampling. Storage times were 28\u00a0days in ambient temperature and at 4\u20138\u00b0C, and 90\u00a0days at \u221220\u00b0C and \u221280\u00b0C. All samples were analyzed regularly during the storage periods. PEth 16:0\/18:1 concentrations were stable (defined as &amp;lt;\u200915% decrease compared with baseline values) at all temperatures up to 28\u00a0days, independent of additive. After 90\u00a0days of storage at \u221220\u00b0C, the mean concentrations had decreased by 18.8% in EDTA tubes and by 13.8% in heparin tubes. At \u221280\u00b0C, the concentrations were stable throughout the 90-day period. The present study shows that in samples containing PEth formed in vivo, PEth 16:0\/18:1 is stable for 28\u00a0days irrespective of storage temperature. During long-term storage, samples should be stored at \u221280\u00b0C.<\/jats:p>","DOI":"10.1093\/jat\/bkaa082","type":"journal-article","created":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T11:11:04Z","timestamp":1596539464000},"page":"417-421","source":"Crossref","is-referenced-by-count":33,"title":["Stability of Phosphatidylethanol 16:0\/18:1 in Freshly Drawn, Authentic Samples from Healthy Volunteers"],"prefix":"10.1093","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3810-0413","authenticated-orcid":false,"given":"Ragnhild Bergene","family":"Skr\u00e5stad","sequence":"first","affiliation":[{"name":"Department of Clinical Pharmacology, St. Olavs Hospital, 7006 Trondheim, Norway"},{"name":"Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, 7491 Trondheim, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olav","family":"Spigset","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, St. Olavs Hospital, 7006 Trondheim, Norway"},{"name":"Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, 7491 Trondheim, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trond Oskar","family":"Aamo","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, St. Olavs Hospital, 7006 Trondheim, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trine Naalsund","family":"Andreassen","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology, St. Olavs Hospital, 7006 Trondheim, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,7,2]]},"reference":[{"key":"2021041212074073500_ref1","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1016\/0006-291X(87)91507-5","article-title":"Formation of phosphatidylethanol in rat brain by phospholipase 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