{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T01:03:16Z","timestamp":1793494996752,"version":"4.5.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2020,8,20]],"date-time":"2020-08-20T00:00:00Z","timestamp":1597881600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Presidency of Council Ministers, Department of Antidrug Policy, Presdency of Council Ministery","award":["no number"],"award-info":[{"award-number":["no number"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,12,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To date, more than 800 molecules are classified as New Psychoactive Substances (NPS), and it is reported that this number increases every year. Whereas several cases of polydrug consumption that led to acute intoxication and death are reported, a lack of effective analytical screening method to detect NPS and classical drug of abuse in human matrices affects the prompt identification of the probable cause of intoxication in emergency department of hospitals. In this concern, a fast, simple and comprehensive high-performance liquid chromatography\u2013tandem mass spectrometry (HPLC\u2013MS-MS) screening method to detect and quantify 77 NPS, 24 classic drugs and 18 related metabolites has been successfully developed and validated in blood, urine and oral fluid. A small volume (100 \u00b5L) of whole blood samples spiked with internal standard deuterated mixture was added to 70 \u00b5L of M3\u00ae buffer, and after precipitation of blood proteins, the supernatant was evaporated to dryness and reconstituted in 1 mL of mobile phase. Same volume (100 \u00b5L) of urine and oral fluid samples spiked with internal standard deuterated mix were only diluted with 500 \u00b5L of M3\u00ae reagent. One microliter of samples of each matrix was injected into HPLC\u2013MS-MS equipment. The run time lasted 10 min with a gradient mobile phase. Mass spectrometric analysis was performed in positive ion multiple reaction monitoring mode. The method was linear for all analytes under investigation with a determination coefficient always better than 0.99. The calibration range for blood and oral fluid was from limits of quantification (LOQs) to 200 ng\/mL, whereas that for urine was LOQs to 1000 ng\/mL. Recovery and matrix effect were always higher than 80%, whereas intra-assay and inter-assay precision were always better than 19% and accuracy was always within 19% of target in every matrix. Applicability of the method was verified by analysis of samples from real cases.<\/jats:p>","DOI":"10.1093\/jat\/bkaa103","type":"journal-article","created":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T11:17:16Z","timestamp":1596799036000},"page":"769-783","source":"Crossref","is-referenced-by-count":48,"title":["A Comprehensive HPLC\u2013MS-MS Screening Method for 77 New Psychoactive Substances, 24 Classic Drugs and 18 Related Metabolites in Blood, Urine and Oral Fluid"],"prefix":"10.1093","volume":"44","author":[{"given":"Annagiulia Di","family":"Trana","sequence":"first","affiliation":[{"name":"Department of Excellence of Biomedical Sciences and Public Health, University \u201cPolitecnica delle Marche\u201d of Ancona, Via Tronto 71, 60124, Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulio","family":"Mannocchi","sequence":"additional","affiliation":[{"name":"University of Camerino, Piazza Cavour 19\/f, 62032, Camerino, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filippo","family":"Pirani","sequence":"additional","affiliation":[{"name":"Department of Excellence of Biomedical Sciences and Public Health, University \u201cPolitecnica delle Marche\u201d of Ancona, Via Tronto 71, 60124, Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nunzia La","family":"Maida","sequence":"additional","affiliation":[{"name":"Department of Excellence of Biomedical Sciences and Public Health, University \u201cPolitecnica delle Marche\u201d of Ancona, Via Tronto 71, 60124, Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Gottardi","sequence":"additional","affiliation":[{"name":"Comedical S.rL, Via Della Cooperazione 27, 38123, Trento, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Pichini","sequence":"additional","affiliation":[{"name":"National Centre on Addiction and Doping, Istituto Superiore di Sanit\u00e0, Viale Regina Elena 299, 00161, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco Paolo","family":"Busard\u00f2","sequence":"additional","affiliation":[{"name":"Department of Excellence of Biomedical Sciences and Public Health, University \u201cPolitecnica delle Marche\u201d of Ancona, Via Tronto 71, 60124, Ancona, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,8,20]]},"reference":[{"key":"2020121209235244200_R1","author":"UNODC Early Warning Advisory on New Psychoactive Substances What are NPS?"},{"key":"2020121209235244200_R2","article-title":"Trends and Developments","author":"European Monitoring Centre for Drugs and Drug Addiction (2019) European Drug Report 2019"},{"key":"2020121209235244200_R3","author":"United Nations Office on Drugs and Crime (UNODC) (2019) Word Drug Report - 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