{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:26:16Z","timestamp":1790799976420,"version":"4.1.0"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,21]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Metformin (MtF) is a treatment used for type 2 diabetes. Lactic acidosis (LA) is a frequent complication that can be either induced by or associated with elevated MtF plasma concentrations. When coupled with a mass spectrometry (MS) system, the probe electrospray ionization (PESI) method allows direct and rapid analysis of different types of matrices without pretreatment. In this study, we developed a PESI\u2013MS method for the determination of MtF in plasma. We used a tandem mass spectrometer equipped with a PESI source in the reaction monitoring mode for the quantitation of MtF. MtF-d6 was chosen as the internal standard (IS), following an isotope dilution (ID) approach. The method was fully validated with six concentration levels (0.5\u201350\u2009mg\/L). The matrix effect was evaluated for each level, and the specificity was tested with a mix of potential co-medications. Using patient samples, the performance was compared with two classical LC\u2013MS-MS and LC\u2013diode array detector (DAD) methods used in external labs. Sample preparation consisted in mixing 10\u2009\u00b5L plasma in 1,000\u2009\u00b5L ethanol\/ammonium formate buffer including MtF-d6 at a fixed concentration of 5\u2009mg\/L. The total run time was 0.31\u2009min. ID gave satisfactory results of accuracy and precision (min\u2013max: \u221212.1 to 15.8% and 1.0\u201317.1%, respectively). The matrix effect was fully corrected by the internal standard (bias\u2009&amp;lt;\u20091%). The specificity study also reported satisfactory results. Finally, in a representative group of 29 patients (55% with a concentration &amp;lt;5\u2009mg\/L, 38% with a concentration &amp;gt;5\u2009mg\/L and 7% not detected), we observed almost identical results when comparing LC\u2013DAD and LC\u2013MS-MS to PESI\u2013MS (r2\u2009&amp;gt;\u20090.99). We propose a specific, sensitive, accurate and ultrafast solution for the measurement of MtF in patient plasma, with no sample preparation or calibration curve building. This could be helpful in a core lab when rapid diagnosis of LA is needed.<\/jats:p>","DOI":"10.1093\/jat\/bkac034","type":"journal-article","created":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T11:24:27Z","timestamp":1654082667000},"page":"89-95","source":"Crossref","is-referenced-by-count":6,"title":["Ultrafast Measurement of Metformin in the Clinical Setting Using Probe Electrospray Ionization Mass Spectrometry"],"prefix":"10.1093","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5171-7923","authenticated-orcid":false,"given":"Pauline","family":"Griffeuille","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Limoges University Hospital , 2 Avenue Martin Luther King, Limoges 87042, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Souleiman","family":"El Balkhi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Limoges University Hospital , 2 Avenue Martin Luther King, Limoges 87042, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Bodeau","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Amiens University Hospital , 1 Rond-Point du Professeur Christian Cabrol, Amiens 80054, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabien","family":"Lamoureux","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Rouen University Hospital , 37 Boulevard Gambetta, Rouen 76000, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Marquet","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Limoges University Hospital , 2 Avenue Martin Luther King, Limoges 87042, France"},{"name":"Pharmacology & Transplantation, UMR1248, INSERM, University of Limoges , Rue du Pr. 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