{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:49:58Z","timestamp":1782434998157,"version":"3.54.5"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T00:00:00Z","timestamp":1666742400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Sir Charles Gairdner Hospital Research Advisory Committee"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,24]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Phosphatidylethanol (PEth) forms in erythrocyte membranes after alcohol consumption, offering a persisting biomarker, that is measurable in whole blood, washed erythrocytes and dried blood spots. For a predominantly erythrocyte-restricted analyte, erythrocyte concentrations seem to have most validity in patients who are anemic through alcoholism or other pathologies, despite preparation increasing assay complexity. Differences in specimen preparation alter PEth concentrations for the same patient, meaning that criteria for interpreting PEth results should relate to specimen type, presenting a barrier to achieving harmonization. We therefore tested whether erythrocyte PEth might be validly calculated by hematocrit correction of a whole blood PEth measurement. PEth testing primarily serves to distinguish drinkers from non-drinkers. In choosing between specimen types, it is important to compare their utility in separating those two groups. We therefore processed 281 blood samples from 17 non-drinkers and 61 drinkers, to prepare matched whole blood and washed erythrocyte specimens. These were assayed by liquid chromatography\u2013tandem mass spectrometry and compared in identifying alcohol consumption. The erythrocyte PEth concentration in the whole blood specimens was also calculated by correcting whole blood concentration by the specimen\u2019s hematocrit, as an alternative to prepare washed erythrocytes. The hematocrit-corrected erythrocyte concentrations were included in these comparisons. Predictably, this work found that sensitivity was consistently better at the lower cut-off of 8\u2009\u00b5g\/L than at 20\u2009\u00b5g\/L. Sensitivities were also higher for washed erythrocytes than whole blood, explained by the lower erythrocyte mass in the same volume of whole blood. Hematocrit-corrected whole blood PEth concentrations correlated with erythrocyte concentrations, except for the four highest values, which did not influence comparative sensitivity. Specificity was 100% for washed erythrocytes, whole blood and hematocrit-corrected whole blood at either cut-off because non-drinkers had undetectable PEth. We conclude that hematocrit correction of whole blood PEth concentrations theoretically provides an alternative to the preparation of washed erythrocytes.<\/jats:p>","DOI":"10.1093\/jat\/bkac084","type":"journal-article","created":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T12:04:32Z","timestamp":1666785872000},"page":"305-310","source":"Crossref","is-referenced-by-count":9,"title":["Hematocrit Correction of Whole Blood Phosphatidylethanol Concentrations to Estimate Erythrocyte PEth Concentrations: Sensitivity, Specificity and Influence on Test Utility"],"prefix":"10.1093","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9487-239X","authenticated-orcid":false,"given":"Daniel","family":"White","sequence":"first","affiliation":[{"name":"Department of Clinical Pharmacology and Toxicology, PathWest Laboratory Medicine , Nedlands, Western Australia 6009, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somayeh","family":"Abbas Zadeh","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology and Toxicology, PathWest Laboratory Medicine , Nedlands, Western Australia 6009, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean","family":"O\u2019Halloran","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology and Toxicology, PathWest Laboratory Medicine , Nedlands, Western Australia 6009, Australia"},{"name":"School of Medicine and School of Biomedical Sciences, University of Western Australia , Crawley, Western Australia 6009, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sam","family":"Salman","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology and Toxicology, PathWest Laboratory Medicine , Nedlands, Western Australia 6009, Australia"},{"name":"School of Medicine and School of Biomedical Sciences, University of Western Australia , Crawley, Western Australia 6009, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A","family":"Joyce","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology and Toxicology, PathWest Laboratory Medicine , Nedlands, Western Australia 6009, Australia"},{"name":"School of Medicine and School of Biomedical Sciences, University of Western Australia , Crawley, Western Australia 6009, Australia"},{"name":"Sir Charles Gairdner Hospital , Nedlands, Western Australia 6009, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,10,26]]},"reference":[{"key":"2023032411255815800_","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1111\/acer.14503","article-title":"Comparison of the diagnostic value of phosphatidylethanol and carbohydrate-deficient transferrin as biomarkers of alcohol consumption","volume":"45","author":"\u00c5rving","year":"2020","journal-title":"Alcoholism: Clinical and 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