{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T22:46:59Z","timestamp":1792018019815,"version":"4.4.0"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Liquid chromatography coupled with mass spectrometry (LC\u2013MS) has been increasingly used for screening purposes in forensic toxicology. High versatility and low time\/resource consumption are the main advantages of this technology. Numerous multi-analyte methods have been validated in order to face the analytical challenge of new psychoactive substances (NPSs). However, forensic toxicologists must focus the attention also on \u201cclassical\u201d NPSs and medicines, such as benzodiazepines (BDZs) and prescription opioids. In this paper, a new method for the simultaneous detection of 169 substances (120 NPSs and 49 other drugs) in hair by LC\u2013MS-MS is described. After the decontamination of hair samples with dichloromethane, a 20-mg aliquot of the sample was mixed with 1\u2009mL of methanol (MeOH; 0.1% of formic acid) and then sonicated at room temperature for 2\u2009h. The mixture was then dried under nitrogen stream and reconstituted with 100\u2009\u00b5L of MeOH. LC separation was achieved with a 100-mm-long C18 column in 35\u2009min, and mass acquisition was performed in dynamic multiple reaction monitoring mode and in positive ionization. The analysis results were very sensitive, with the limit of quantification ranging from 0.07 to 10.0\u00a0pg\/mg. Accuracy and precision were always within the acceptable criteria. Matrix effect and recovery rate ranges were from \u221221.3 to +\u200921.9% and from 75.0 to 99.3%, respectively. The new method was successfully applied in a preliminary study on the prevalence of NPSs, BDZs and other substances in case of driving license issuance. In 14% of cases, BDZs\/antidepressants (mainly trazodone, diazepam\/nordiazepam and flunitrazepam) were found. Codeine, ketamine, methylone and mephedrone were also detected.<\/jats:p>","DOI":"10.1093\/jat\/bkac093","type":"journal-article","created":{"date-parts":[[2022,11,30]],"date-time":"2022-11-30T11:07:44Z","timestamp":1669806464000},"page":"e262-e273","source":"Crossref","is-referenced-by-count":25,"title":["A New Multi-Analyte LC\u2013MS-MS Screening Method for the Detection of 120 NPSs and 49 Drugs in Hair"],"prefix":"10.1093","volume":"46","author":[{"given":"J","family":"Rubicondo","sequence":"first","affiliation":[{"name":"Forensic Toxicology Unit, Department of Health Science, University of Florence , L.go Brambilla 3, Florence 50134, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Scuffi","sequence":"additional","affiliation":[{"name":"Forensic Toxicology Unit, Department of Health Science, University of Florence , L.go Brambilla 3, Florence 50134, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Pietrosemoli","sequence":"additional","affiliation":[{"name":"Forensic Toxicology Unit, Department of Health Science, University of Florence , L.go Brambilla 3, Florence 50134, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Mineo","sequence":"additional","affiliation":[{"name":"Forensic Toxicology Unit, Department of Health Science, University of Florence , L.go Brambilla 3, Florence 50134, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Terranova","sequence":"additional","affiliation":[{"name":"Forensic Toxicology Unit, Department of Health Science, University of Florence , L.go Brambilla 3, Florence 50134, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Bartucca","sequence":"additional","affiliation":[{"name":"Forensic Toxicology Unit, Department of Health Science, University of Florence , L.go Brambilla 3, Florence 50134, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Trignano","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Sassari , Viale San Pietro 43c, Sassari 07100, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Bertol","sequence":"additional","affiliation":[{"name":"U.R.I.To.N.\u2014Unit of Research, University of Florence , L.go Brambilla 3, Florence 50134, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9256-4326","authenticated-orcid":false,"given":"F","family":"Vaiano","sequence":"additional","affiliation":[{"name":"Forensic Toxicology Unit, Department of Health Science, University of Florence , L.go Brambilla 3, Florence 50134, Italy"},{"name":"U.R.I.To.N.\u2014Unit of Research, University of Florence , L.go Brambilla 3, Florence 50134, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,12,1]]},"reference":[{"key":"2023012411403639000_R1","article-title":"European drug report: trends and developments","volume-title":"European drug report: trends and developments","author":"European Monitoring Centre for Drugs and Drug Addiction","year":"2021"},{"key":"2023012411403639000_R2","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/0378-4347(92)80526-V","article-title":"Systematic toxicological analysis of drugs and their metabolites by gas chromatography-mass spectrometry","volume":"580","author":"Maurer","year":"1992","journal-title":"Journal of Chromatography"},{"key":"2023012411403639000_R3","doi-asserted-by":"publisher","first-page":"3074","DOI":"10.1002\/jssc.200900092","article-title":"General unknown screening procedure for the characterization of human drug metabolites in forensic toxicology: applications and constraints","volume":"32","author":"Sauvage","year":"2009","journal-title":"Journal of Separation Science"},{"key":"2023012411403639000_R4","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/S0378-4347(99)00265-0","article-title":"Chromatographic screening techniques in systematic toxicological analysis","volume":"733","author":"Drummer","year":"1999","journal-title":"Journal of Chromatography B: Biomedical Sciences and Applications"},{"key":"2023012411403639000_R5","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1080\/14756366.2017.1333987","article-title":"Advances in new psychoactive substances identification: the U.R.I.To.N. 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