{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T15:17:34Z","timestamp":1780413454034,"version":"3.54.1"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2022,12,14]],"date-time":"2022-12-14T00:00:00Z","timestamp":1670976000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,24]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>New psychoactive substances (NPSs) still represent an issue of great concern worldwide despite efforts made by national and international control systems to limit the spread of these substances. Alpha-pyrrolidinohexanophenone (\u03b1-PHP) is a fairly recent synthetic cathinone (the second largest group of monitored substances in Europe) with only a few published studies on the substance. Though there is a low incidence of NPS consumption in Portugal, a recent increase in apprehensions and detections in biological matrices of the substance was verified. An analytical methodology was developed and validated for determining and quantitating \u03b1-PHP in blood. Solid-phase extraction was employed for sample preparation (500\u2009\u03bcL), which was further analyzed by gas chromatography--mass spectrometry-electron ionization in single-ion monitoring mode with cocaine-d3 as the internal standard. Method validation followed the guidelines of the American National Standards Institute\/AAFS Standards Board (ANSI\/ASB Standard 036). The procedure was linear between 10 and 1,000\u2009ng\/mL, with determination coefficients (r2) higher than 0.999. Carryover was not observed. A limit of detection of 5\u2009ng\/mL and a limit of quantitation of 10\u2009ng\/mL were achieved. Intraday and intermediate precision and bias assessment showed satisfactory results (coefficient of variation &amp;lt;17.7%; bias &amp;lt;11.6%), and extraction efficiency ranged from 98.5% to 103.3%. The stability of the substance was considered acceptable for at least 6\u2009h at room temperature, 48\u2009h in the autosampler and 21\u2009days after five freeze\/thaw cycles. The developed methodology was applied to 15 real samples from the Laboratory of Chemistry and Forensic Toxicology, Centre Branch of the National Institute of Legal Medicine and Forensic Sciences, Portugal, with drug concentrations ranging from 15 to 227\u2009ng\/mL. Available information for each case is also detailed in the present article.<\/jats:p>","DOI":"10.1093\/jat\/bkac104","type":"journal-article","created":{"date-parts":[[2022,12,14]],"date-time":"2022-12-14T18:44:54Z","timestamp":1671043494000},"page":"271-279","source":"Crossref","is-referenced-by-count":9,"title":["Development and Validation of a GC\u2013MS-EI Method to Determine \u03b1-PHP in Blood: Application to Samples Collected during Medico-Legal Autopsies"],"prefix":"10.1093","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9277-2424","authenticated-orcid":false,"given":"Francisca","family":"Machado","sequence":"first","affiliation":[{"name":"Servi\u00e7o de Qu\u00edmica e Toxicologia Forenses, Instituto Nacional de Medicina Legal e Ci\u00eancias Forenses\u2014Delega\u00e7\u00e3o do Centro, P\u00f3lo das Ci\u00eancias da Sa\u00fade (P\u00f3lo III) , Azinhaga de Santa Comba, Coimbra 3000-548, Portugal"},{"name":"Faculdade de Medicina, Universidade de Coimbra, P\u00f3lo das Ci\u00eancias da Sa\u00fade (P\u00f3lo III) , Azinhaga de Santa Comba, Coimbra 3000-548, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o","family":"Franco","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Qu\u00edmica e Toxicologia Forenses, Instituto Nacional de Medicina Legal e Ci\u00eancias Forenses\u2014Delega\u00e7\u00e3o do Sul , Rua Manuel Bento de Sousa, 3, Lisboa 1169-201, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duarte Nuno","family":"Vieira","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina, Universidade de Coimbra, P\u00f3lo das Ci\u00eancias da Sa\u00fade (P\u00f3lo III) , Azinhaga de Santa Comba, Coimbra 3000-548, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1661-0367","authenticated-orcid":false,"given":"Cl\u00e1udia","family":"Margalho","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Qu\u00edmica e Toxicologia Forenses, Instituto Nacional de Medicina Legal e Ci\u00eancias Forenses\u2014Delega\u00e7\u00e3o do Centro, P\u00f3lo das Ci\u00eancias da Sa\u00fade (P\u00f3lo III) , Azinhaga de Santa Comba, Coimbra 3000-548, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,12,14]]},"reference":[{"key":"2023032411262858600_","article-title":"World Drug Report 2022: Set of 5 Booklets","author":"United Nations Office on Drugs and Crime","year":"2022"},{"key":"2023032411262858600_","doi-asserted-by":"crossref","first-page":"35","DOI":"10.7748\/ns.28.52.35.e8850","article-title":"A critical analysis of national policy relating to legal 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