{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T06:40:48Z","timestamp":1757313648278,"version":"3.37.3"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T00:00:00Z","timestamp":1671667200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18H04079"],"award-info":[{"award-number":["18H04079"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,4,14]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Methanol poisoning is caused by the toxicity of formate, a by-product of methanol metabolism. Measurement of blood formate concentrations is required for emergency treatment and investigation of the cause of death. In this study, we measured concentrations of formate in the plasma of a patient with methanol poisoning using headspace gas chromatography--mass spectrometry (HS-GC--MS) and a formate assay kit. Results showed a discrepancy as the quantitative values of the kit were higher than those of HS-GC--MS. Metabolic profiling of low-molecular-weight organic compounds in patient plasma samples showed that the concentrations of lactate were correlated with the values obtained using the kit. We observed a progression when lactate and lactate dehydrogenase were added to the kit reaction simultaneously, even in the absence of formate. Moreover, disulfiram, an aldehyde dehydrogenase inhibitor, suppressed the values of patient plasma samples in the formate assay kit, implying that formate production from remaining methanol in patient plasma samples via formaldehyde occurred during the kit reaction. The reactions of the kit with lactate and methanol were undesirable for accurate measurement of formate concentration in the sample. However, considering that elevated concentrations of lactate and remaining methanol both cause acidosis and are dangerous to the body, cross-reactions with lactate and methanol in the formate assay kit may be acceptable for rapid diagnosis in facilities where HS-GC--MS and other physical and chemical equipment are unavailable.<\/jats:p>","DOI":"10.1093\/jat\/bkac107","type":"journal-article","created":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T13:02:47Z","timestamp":1671714167000},"page":"338-345","source":"Crossref","is-referenced-by-count":3,"title":["Comparison of Quantitative Values of Headspace Gas Chromatography--Mass Spectrometry and a Formate Quantification Kit in Blood Formate Quantification"],"prefix":"10.1093","volume":"47","author":[{"given":"Haruhi","family":"Yoshida","sequence":"first","affiliation":[{"name":"Cause of Death Investigation in Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, Osaka University , 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9348-1629","authenticated-orcid":false,"given":"Kazuo","family":"Harada","sequence":"additional","affiliation":[{"name":"Cause of Death Investigation in Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, Osaka University , 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan"},{"name":"Department of Legal Medicine, Graduate School of Medicine, Osaka University , 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukari","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Cause of Death Investigation in Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, Osaka University , 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jumpei","family":"Yoshimura","sequence":"additional","affiliation":[{"name":"Department of Traumatology and Acute Critical Medicine, Graduate School of Medicine, Osaka University , 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Shimazu","sequence":"additional","affiliation":[{"name":"Department of Traumatology and Acute Critical Medicine, Graduate School of Medicine, Osaka University , 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan"},{"name":"Osaka General Medical Center , 3-1-56 Bandaihigashi, Sumiyoshi-Ku, Osaka 558-8558, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Department of Legal Medicine, Graduate School of Medicine, Osaka University , 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,12,22]]},"reference":[{"article-title":"Global status report on alcohol and health","year":"2011","author":"World Health Organization","key":"2023041410544456000_"},{"key":"2023041410544456000_","doi-asserted-by":"crossref","DOI":"10.3390\/foods10071625","article-title":"Illicit alcohol: public health risk of methanol poisoning and policy mitigation strategies","volume":"10","author":"Manning","year":"2021","journal-title":"Foods"},{"key":"2023041410544456000_","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1007\/s11739-018-1799-9","article-title":"Toxic alcohol diagnosis and management: an emergency medicine review","volume":"13","author":"Ng","year":"2018","journal-title":"Internal and Emergency Medicine"},{"key":"2023041410544456000_","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1081\/CLT-120006753","article-title":"Methanol poisoning exposures in the United States: 1993-1998","volume":"40","author":"Davis","year":"2002","journal-title":"Journal of Toxicology Clinical Toxicology"},{"key":"2023041410544456000_","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1111\/j.1600-0773.1991.tb01290.x","article-title":"Methanol and formic acid toxicity: biochemical mechanisms","volume":"69","author":"Liesivuori","year":"1991","journal-title":"Pharmacology & Toxicology"},{"key":"2023041410544456000_","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1056\/NEJMra1615295","article-title":"Toxic alcohols","volume":"378","author":"Kraut","year":"2002","journal-title":"The New England Journal of Medicine"},{"key":"2023041410544456000_","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1111\/bcpt.12338","article-title":"Is the measurement of serum formate concentration useful in the diagnostics of acute methanol poisoning? 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