{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:56:29Z","timestamp":1787316989395,"version":"build-2736575974"},"reference-count":9,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T00:00:00Z","timestamp":1719446400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,21]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Brain can be a useful specimen for toxicology testing as it is a protected and isolated organ with lower metabolic activity than other tissues, but there is currently no published data supporting the stability of stimulant drugs in prepared brain homogenates. Brain homogenates were evaluated to determine the stability of the following stimulant drugs: amphetamine, benzoylecgonine, bupropion, cocaethylene, cocaine, ephedrine, methylenedioxyamphetamine, methylenedioxymethamphetamine, methamphetamine, and phentermine. Four different homogenates were prepared at a 1:4 dilution with deionized water and fortified at 500\u2009ng\/mL of: cocaine without sodium fluoride, cocaine with 1% sodium fluoride, stimulant drugs other than cocaine without sodium fluoride, and stimulant drugs other than cocaine with 1% sodium fluoride. The fortified homogenates were aliquoted into 13\u2009\u00d7\u2009100-mm screw cap tubes and stored at room temperature (\u223c20\u00b0C), refrigerated (2\u20138\u00b0C), or frozen (&amp;lt;\u22125\u00b0C) and analyzed in triplicate on Days 0, 1, 3, 7, 14, 30, 60, and 90. Analytes were considered stable as long as the difference in analyte\/internal standard response ratio from Day 0 was less than 20% and the peaks met qualitative acceptance criteria. All analytes were stable for up to 90\u2009days when stored frozen with or without sodium fluoride and had variable stability at all other evaluated conditions.<\/jats:p>","DOI":"10.1093\/jat\/bkae058","type":"journal-article","created":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T23:59:41Z","timestamp":1719532781000},"page":"514-518","source":"Crossref","is-referenced-by-count":3,"title":["Brain homogenate stability for stimulant drugs"],"prefix":"10.1093","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8324-5807","authenticated-orcid":false,"given":"Grayce","family":"Behnke","sequence":"first","affiliation":[{"name":"Forensic Toxicology Laboratory, Harris County Institute of Forensic Sciences , 1861 Old Spanish Trail, Houston, TX 77954, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa R","family":"Gray","sequence":"additional","affiliation":[{"name":"Forensic Toxicology Laboratory, Harris County Institute of Forensic Sciences , 1861 Old Spanish Trail, Houston, TX 77954, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3980-233X","authenticated-orcid":false,"given":"Crystal","family":"Arndt","sequence":"additional","affiliation":[{"name":"Forensic Toxicology Laboratory, Harris County Institute of Forensic Sciences , 1861 Old Spanish Trail, Houston, TX 77954, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,6,27]]},"reference":[{"key":"2024082121410027500_R1","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1093\/jat\/bku139","article-title":"Brain tissue: a viable postmortem toxicological specimen","volume":"39","author":"Rohrig","year":"2015","journal-title":"J Anal Toxicol"},{"key":"2024082121410027500_R2","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-030-42917-1","volume-title":"Principles of Forensic 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cocaine in horse blood, sheep vitreous and deer muscle","volume":"217","author":"Rees","year":"2012","journal-title":"Forensic Sci Int"}],"container-title":["Journal of Analytical 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