{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T11:53:16Z","timestamp":1791978796953,"version":"4.4.0"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100031597","name":"National Forensic Service","doi-asserted-by":"publisher","award":["NFS2026ND01"],"award-info":[{"award-number":["NFS2026ND01"]}],"id":[{"id":"10.13039\/100031597","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012261","name":"Ministry of the Interior and Safety","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012261","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Novel psychoactive phenethylamines and phenidates are synthetically designed analogs of conventional substances such as 3,4-methylenedioxymethamphetamine (MDMA), amphetamine, and methylphenidate, developed to circumvent existing drug regulations. In Korea, the abuse of these substances has increased, particularly among habitual users and newly addicted younger individuals. To address this issue, new substances are regulated through the analog legislation and the temporary narcotic designation system. In this study, liquid chromatography\u2013tandem mass spectrometry method using solid-phase extraction was developed and validated for the simultaneous determination of 13 substituted phenethylamines and 5 substituted phenidates in human urine. The target analytes included 5-MAPDB, BDB, 4-FEA, PMEA, 3F-Phenetrazine, 25iP-NBOMe, 2C-N, 2C-TFM, 30C-NBOMe, 3C-P, 4-MMA-NBOMe, DOI, and 2C-H, as well as 4-MeTMP, 4F-EPH, 4F-MPH, IPPH, and HDEP-28. No interfering peaks were observed at the retention times of analytes and internal standards. Linearity was confirmed over analyte-specific concentration within the overall range of 0.1\u2013100 ng\/ml, with coefficient of determination (r2) exceeding 0.995. The limits of detection and quantification were 0.001\u20130.1 and 0.1\u20130.2 ng\/ml, respectively. Accuracy and precision, expressed as bias and coefficient of variation (CV), were within \u00b110% and &amp;lt;10%, respectively. The CVs for matrix effects and extraction efficiencies were approximately 10%, and the extraction efficiencies exceeded 70%, confirming the reliability of the method for forensic applications. The validated method enables simultaneous determination of substituted phenethylamines and phenidates in urine and facilitates prompt positive identification in forensic casework. In addition, the structural information, multiple reaction monitoring conditions, and analytical method validation results presented in this study can serve as a basis for expanding research on substances with similar core structures, which is expected to strengthen regulatory responses to evolving drug trends.<\/jats:p>","DOI":"10.1093\/jat\/bkag086","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T11:37:30Z","timestamp":1790077050000},"source":"Crossref","is-referenced-by-count":0,"title":["Development and validation of an liquid chromatography\u2013tandem mass spectrometry method for the detection of substituted phenethylamines and phenidates designated as temporary narcotics in urine"],"prefix":"10.1093","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3261-5045","authenticated-orcid":false,"given":"Sujin","family":"Jeong","sequence":"first","affiliation":[{"name":"Toxicology and Narcotics Division, Seoul Institute, National Forensic Service , Seoul 08036,","place":["South Korea"]},{"name":"College of Pharmacy, Chungnam National University , Daejeon 34134,","place":["South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoseob","family":"Kim","sequence":"additional","affiliation":[{"name":"Jeju Branch, National Forensic Service , Jeju-do 63309,","place":["South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seojin","family":"Kang","sequence":"additional","affiliation":[{"name":"Toxicology and Narcotics Division, Seoul Institute, National Forensic Service , Seoul 08036,","place":["South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chae Eun","family":"Lee","sequence":"additional","affiliation":[{"name":"Toxicology and Narcotics Division, Seoul Institute, National Forensic Service , Seoul 08036,","place":["South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moonhee","family":"Jang","sequence":"additional","affiliation":[{"name":"Toxicology and Narcotics Division, Seoul Institute, National Forensic Service , Seoul 08036,","place":["South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7488-0539","authenticated-orcid":false,"given":"Hyung Min","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Chungnam National University , Daejeon 34134,","place":["South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,22]]},"reference":[{"key":"2026101407315767300_bkag086-B1","doi-asserted-by":"crossref","first-page":"w14043","DOI":"10.4414\/smw.2015.14043","article-title":"Novel psychoactive substances: classification, pharmacology, and toxicology","volume":"145","author":"Liechti","year":"2015","journal-title":"Swiss Med Wkly"},{"key":"2026101407315767300_bkag086-B2","volume-title":"Novel Psychoactive Substances: Classification, Pharmacology and Toxicology","author":"Dargan","year":"2013"},{"key":"2026101407315767300_bkag086-B3","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1176\/ajp.155.10.1325","article-title":"Dopamine transporter occupancies in the human brain induced by therapeutic doses of oral methylphenidate","volume":"155","author":"Volkow","year":"1998","journal-title":"Am J Psychiatry"},{"key":"2026101407315767300_bkag086-B4","doi-asserted-by":"crossref","first-page":"705","DOI":"10.3109\/15563650.2011.615318","article-title":"Clinical toxicology of newer recreational drugs","volume":"49","author":"Hill","year":"2011","journal-title":"Clin Toxicol (Phila)"},{"key":"2026101407315767300_bkag086-B5","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1038\/npp.2015.260","article-title":"Abuse of new psychoactive substances: threats and solutions","volume":"41","author":"Baumann","year":"2016","journal-title":"Neuropsychopharmacology"},{"key":"2026101407315767300_bkag086-B6","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1002\/dta.319","article-title":"A brief history of \u201cnew psychoactive substances\u201d","volume":"3","author":"King","year":"2011","journal-title":"Drug Test Anal"},{"key":"2026101407315767300_bkag086-B7","doi-asserted-by":"crossref","first-page":"1668","DOI":"10.1016\/S0140-6736(19)32231-7","article-title":"New psychoactive substances: challenges for drug surveillance, control, and public health responses","volume":"394","author":"Peacock","year":"2019","journal-title":"Lancet"},{"key":"2026101407315767300_bkag086-B8","author":"United Nations Office on Drugs and Crime","year":"2024"},{"key":"2026101407315767300_bkag086-B9","doi-asserted-by":"crossref","first-page":"12","DOI":"10.54299\/tmed\/msot8578","article-title":"NBOMe toxicity and fatalities: a review of the literature","volume":"1","author":"Lipow","year":"2022","journal-title":"Transform Med"},{"key":"2026101407315767300_bkag086-B10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11419-022-00638-6","article-title":"Analysis of 2, 5-dimethoxy-amphetamines and 2, 5-dimethoxy-phenethylamines aiming their determination in biological matrices: a review","volume":"41","author":"Nieddu","year":"2023","journal-title":"Forensic Toxicol"},{"key":"2026101407315767300_bkag086-B11","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1007\/s00414-024-03236-w","article-title":"Alpha-methyltryptamine and 5-(2-methylaminopropyl)-benzofuran (5-MAPB) fatal co-intoxication: case report and review of literature","volume":"138","author":"Epain","year":"2024","journal-title":"Int J Legal Med"},{"key":"2026101407315767300_bkag086-B12","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1093\/jat\/bkx060","article-title":"Multiple drug-toxicity involving novel psychoactive substances, 3-fluorophenmetrazine and U-47700","volume":"41","author":"Ellefsen","year":"2017","journal-title":"J Anal Toxicol"},{"key":"2026101407315767300_bkag086-B13","author":"Advisory Council on the Misuse of Drugs","year":"2017"},{"key":"2026101407315767300_bkag086-B14","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1177\/19253621221142480","article-title":"First death involving 4-fluoroethylphenidate (4F-EPH): case report, user experiences, and review of the related literature","volume":"12","author":"Corkery","year":"2022","journal-title":"Acad Forensic Pathol"},{"key":"2026101407315767300_bkag086-B15","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1093\/jat\/bkz061","article-title":"4-Fluoromethylphenidate: fatal intoxication involving a previously unreported novel psychoactive substance in the USA","volume":"43","author":"Shoff","year":"2019","journal-title":"J Anal Toxicol"},{"key":"2026101407315767300_bkag086-B16","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.clinbiochem.2005.01.014","article-title":"Multi-analyte procedures for screening for and quantification of drugs in blood, plasma, or serum by liquid chromatography-single stage or tandem mass spectrometry (LC-MS or LC-MS\/MS) relevant to clinical and forensic toxicology","volume":"38","author":"Maurer","year":"2005","journal-title":"Clin Biochem"},{"key":"2026101407315767300_bkag086-B17","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1097\/00007691-200404000-00020","article-title":"Detection times of drugs of abuse in blood, urine, and oral fluid","volume":"26","author":"Verstraete","year":"2004","journal-title":"Ther Drug Monit"},{"key":"2026101407315767300_bkag086-B18","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.chroma.2015.04.002","article-title":"Simultaneous determination of 40 novel psychoactive stimulants in urine by liquid chromatography\u2013high resolution mass spectrometry and library matching","volume":"1397","author":"Concheiro","year":"2015","journal-title":"J Chromatogr A"},{"key":"2026101407315767300_bkag086-B19","doi-asserted-by":"crossref","first-page":"110884","DOI":"10.1016\/j.forsciint.2021.110884","article-title":"Simultaneous LC-MS\/MS screening for multiple phenethylamine-type conventional drugs and new psychoactive substances in urine","volume":"325","author":"Fan","year":"2021","journal-title":"Forensic Sci Int"},{"key":"2026101407315767300_bkag086-B20","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1093\/jat\/bkz052","article-title":"Use of UPLC-HRMS\/MS for in vitro and in vivo metabolite identification of three methylphenidate-derived new psychoactive substances","volume":"44","author":"Manier","year":"2020","journal-title":"J Anal Toxicol"},{"key":"2026101407315767300_bkag086-B21","doi-asserted-by":"crossref","first-page":"115082","DOI":"10.1016\/j.jpba.2022.115082","article-title":"A multi-analyte LC-MS\/MS method for the determination of 57 pharmaceuticals and illicit drugs in plasma, and its application to poisoning cases","volume":"222","author":"Dos Santos","year":"2023","journal-title":"J Pharm Biomed Anal"},{"key":"2026101407315767300_bkag086-B22","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/s00228-019-02787-x","article-title":"Pharmacokinetics of methylphenidate and ritalinic acid in plasma correlations with exhaled breath and oral fluid in healthy volunteers","volume":"76","author":"Arvidsson","year":"2020","journal-title":"Eur J Clin Pharmacol"},{"key":"2026101407315767300_bkag086-B23","doi-asserted-by":"crossref","first-page":"e262","DOI":"10.1093\/jat\/bkac093","article-title":"A new multi-analyte LC\u2013MS-MS screening method for the detection of 120 NPSs and 49 drugs in hair","volume":"46","author":"Rubicondo","year":"2023","journal-title":"J Anal Toxicol"},{"key":"2026101407315767300_bkag086-B24","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1093\/jat\/bkab014","article-title":"Simultaneous quantitation of seven phenethylamine-type drugs in forensic blood and urine samples by UHPLC\u2013MS-MS","volume":"46","author":"Yang","year":"2022","journal-title":"J Anal Toxicol"},{"key":"2026101407315767300_bkag086-B25","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1002\/dta.3170","article-title":"Development and validation of a rapid LC-MS\/MS method for the detection of 182 novel psychoactive substances in whole blood","volume":"14","author":"Giorgetti","year":"2022","journal-title":"Drug Test Anal"},{"key":"2026101407315767300_bkag086-B26","doi-asserted-by":"crossref","first-page":"221","DOI":"10.3390\/separations8110221","article-title":"Development of a new LC-MS\/MS screening method for detection of 120 NPS and 43 drugs in blood","volume":"8","author":"Vaiano","year":"2021","journal-title":"Separations"},{"key":"2026101407315767300_bkag086-B27","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.forsciint.2006.05.021","article-title":"Validation of new methods","volume":"165","author":"Peters","year":"2007","journal-title":"Forensic Sci Int"},{"key":"2026101407315767300_bkag086-B28","doi-asserted-by":"crossref","first-page":"3019","DOI":"10.1021\/ac020361s","article-title":"Strategies for the assessment of matrix effect in quantitative bioanalytical methods based on HPLC\u2212MS\/MS","volume":"75","author":"Matuszewski","year":"2003","journal-title":"Anal Chem"},{"key":"2026101407315767300_bkag086-B29","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1007\/s00769-011-0775-0","article-title":"Practical aspects concerning validation and quality control for forensic and clinical bioanalytical quantitative methods","volume":"16","author":"Wille","year":"2011","journal-title":"Accred Qual Assur"},{"key":"2026101407315767300_bkag086-B30","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1093\/jat\/28.6.407","article-title":"A general screening and confirmation approach to the analysis of designer tryptamines and phenethylamines in blood and urine using GC-EI-MS and HPLC-electrospray-MS","volume":"28","author":"Vorce","year":"2004","journal-title":"J Anal Toxicol"},{"key":"2026101407315767300_bkag086-B31","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1093\/jat\/26.6.347","article-title":"Extraction of amphetamine and methamphetamine from urine specimens with Cerex Polycrom\u2122 Clin II solid-phase extraction columns and the Speedisk\u2122 48 pressure processor","volume":"26","author":"Churley","year":"2002","journal-title":"J Anal Toxicol"},{"key":"2026101407315767300_bkag086-B32","author":"National Center for Biotechnology Information"},{"key":"2026101407315767300_bkag086-B33","author":"National Center for Biotechnology Information"},{"key":"2026101407315767300_bkag086-B34","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1093\/jat\/33.8.439","article-title":"Urinary MDMA, MDA, HMMA, and HMA excretion following controlled MDMA administration to humans","volume":"33","author":"Abraham","year":"2009","journal-title":"J Anal Toxicol"},{"key":"2026101407315767300_bkag086-B35","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1093\/clinchem\/48.10.1703","article-title":"Duration of detectable methamphetamine and amphetamine excretion in urine after controlled oral administration of methamphetamine to humans","volume":"48","author":"Oyler","year":"2002","journal-title":"Clin Chem"},{"key":"2026101407315767300_bkag086-B36","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1093\/jat\/bkae097","article-title":"Drug detection in oral fluid and urine after single therapeutic doses of dexamphetamine, lisdexamphetamine, and methylphenidate in healthy volunteers","volume":"49","author":"Helland","year":"2025","journal-title":"J Anal Toxicol"}],"container-title":["Journal of Analytical Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jat\/advance-article-pdf\/doi\/10.1093\/jat\/bkag086\/71322687\/bkag086.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jat\/article-pdf\/50\/9\/bkag086\/71322687\/bkag086.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jat\/article-pdf\/50\/9\/bkag086\/71322687\/bkag086.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T11:35:56Z","timestamp":1791977756000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jat\/article\/doi\/10.1093\/jat\/bkag086\/8829347"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":36,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9,26]]}},"URL":"https:\/\/doi.org\/10.1093\/jat\/bkag086","relation":{},"ISSN":["1945-2403"],"issn-type":[{"value":"1945-2403","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,9]]},"published":{"date-parts":[[2026,9]]},"article-number":"bkag086"}}