{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T03:27:26Z","timestamp":1762918046558,"version":"3.37.3"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2020,1,21]],"date-time":"2020-01-21T00:00:00Z","timestamp":1579564800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001809","name":"NSFC","doi-asserted-by":"publisher","award":["81172906"],"award-info":[{"award-number":["81172906"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Key Technology R & D Program of China","award":["2007BAK26B05","2012BAK02B02"],"award-info":[{"award-number":["2007BAK26B05","2012BAK02B02"]}]},{"name":"National Science and Technology Special Project","award":["SQ2015FYJ010051"],"award-info":[{"award-number":["SQ2015FYJ010051"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,5,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Diazepam abuse is widespread all over the word, leading to an increasing number of forensic cases such as suicide, drug-driving and robbery, but relevant studies are limited regarding the extraction of diazepam and its metabolites in oral fluid. This study aimed to investigate the pharmacokinetics of diazepam and its metabolites in oral fluid after a single oral dose in healthy volunteers. There was a total of 28 volunteers, and each ingested 5\u00a0mg diazepam orally, then ~2\u00a0mL oral fluid were collected from each participant at post-consumption time-points of prior (zero), 1, 2, 4, 8, 12, 24\u00a0h and 2, 3, 6, 12 and 15 days, respectively. All samples were extracted with solid-phase extraction and analyzed with high-performance liquid chromatography-tandem mass spectrometry method, and diazepam and nordazepam were detected in the oral fluid of volunteers. Pharmacokinetics of diazepam in oral fluid conformed to a two-compartment model, and k01_HL, k12_HL, k10_HL were 0.7\u00a0\u00b1\u00a01.1, 31.4\u00a0\u00b1\u00a068.5, 12.1\u00a0\u00b1\u00a011.6\u00a0h, respectively, nordazepam conformed to an one-compartment model, and k01_HL, k10_HL were 41.5\u00a0\u00b1\u00a044.8, 282.3\u00a0\u00b1\u00a0365.5\u00a0h, respectively. Both diazepam and nordazepam could be detected continuously for 15\u00a0days, although there were individual differences, and the results regarding diazepam detecting in oral fluid will be of much help in forensic science and drug screening filed.<\/jats:p>","DOI":"10.1093\/jat\/bkz101","type":"journal-article","created":{"date-parts":[[2020,1,22]],"date-time":"2020-01-22T07:06:42Z","timestamp":1579676802000},"page":"348-353","source":"Crossref","is-referenced-by-count":5,"title":["An Experimental Pharmacokinetics Study of Diazepam and Its Metabolites in Oral Fluid of Chinese Population"],"prefix":"10.1093","volume":"44","author":[{"given":"Le-le","family":"Wang","sequence":"first","affiliation":[{"name":"School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin-xin","family":"Ren","sequence":"first","affiliation":[{"name":"Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"He","sequence":"first","affiliation":[{"name":"Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guan-feng","family":"Cui","sequence":"first","affiliation":[{"name":"Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi-wen","family":"Wei","sequence":"first","affiliation":[{"name":"School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke-ming","family":"Yun","sequence":"first","affiliation":[{"name":"School of Forensic Medicine, Shanxi Medical University, Taiyuan 030001, 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