{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T15:46:24Z","timestamp":1771861584937,"version":"3.50.1"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"6","funder":[{"name":"National Key Scientific Project for New Drug Discovery and Development of China","award":["2013ZX09301305-002"],"award-info":[{"award-number":["2013ZX09301305-002"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81202855"],"award-info":[{"award-number":["81202855"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013806","name":"Shenyang Pharmaceutical University","doi-asserted-by":"publisher","award":["ZQN2016013"],"award-info":[{"award-number":["ZQN2016013"]}],"id":[{"id":"10.13039\/501100013806","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,12,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Anti-tumour-analgesic peptide (AGAP), one scorpion toxin purified from Buthus martensii Karsch, was known as its analgesic and anti-tumour activities. Trp38, a conserved aromatic residue of AGAP, might play important roles in its interaction with sodium channels. In this study, a mutant W38F was generated and effects of W38F were examined on hNav1.4, hNav1.5 and hNav1.7 by using whole-cell patch-clamp, which were closely associated to the biotoxicity of skeletal and cardiac muscles and pain signalling. The data showed that W38F decreased the inhibition effects of peak currents of hNav1.7, hNav1.4 and hNav1.5 compared with AGAP, notably, W38F reduced the analgesic activity compared with AGAP. The results suggested that Trp38 be a crucial amino acid involved in the interaction with these three sodium channels. The decreased analgesic activity of W38F might result from its much less inhibition of hNav1.7. These findings provided more information about the relationship between structure and function of AGAP and may facilitate the modification of other scorpion toxins with pharmacological effects.<\/jats:p>","DOI":"10.1093\/jb\/mvaa088","type":"journal-article","created":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T11:13:48Z","timestamp":1595502828000},"page":"633-641","source":"Crossref","is-referenced-by-count":6,"title":["Trp: a conserved aromatic residue crucial to the interaction of a scorpion peptide with sodium channels"],"prefix":"10.1093","volume":"168","author":[{"given":"Yijia","family":"Xu","sequence":"first","affiliation":[{"name":"School of Life Sciences and Biopharmaceutical Science, Shenyang Pharmaceutical University, 103 Wenhua road, Shenyang, Liaoning 110016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianfang","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Life and Health Sciences, Northeastern University, 195 Chuangxin road, Shenyang, Liaoning 110004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua road, Shenyang, Liaoning 110016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohua","family":"Kong","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Biopharmaceutical Science, Shenyang Pharmaceutical University, 103 Wenhua road, Shenyang, Liaoning 110016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangxue","family":"Meng","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Biopharmaceutical Science, Shenyang Pharmaceutical University, 103 Wenhua road, Shenyang, Liaoning 110016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanfeng","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Biopharmaceutical Science, Shenyang Pharmaceutical University, 103 Wenhua road, Shenyang, Liaoning 110016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Cui","sequence":"additional","affiliation":[{"name":"School of Medical Devices, Shenyang Pharmaceutical University, 103 Wenhua road, Shenyang, Liaoning 110016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Su","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Shengjing Hospital of China Medical University, 36 Sanhao street, Shenyang, Liaoning 110004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mingyi","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Biopharmaceutical Science, Shenyang Pharmaceutical University, 103 Wenhua road, Shenyang, Liaoning 110016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinghai","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Medical Devices, Shenyang Pharmaceutical University, 103 Wenhua road, Shenyang, Liaoning 110016, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,7,30]]},"reference":[{"key":"2020122606294457400_mvaa088-B1","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/S1046-5928(02)00609-5","article-title":"Expression of an antitumor-analgesic peptide from the venom of Chinese scorpion Buthus martensii Karsch in Escherichia coli","volume":"27","author":"Liu","year":"2003","journal-title":"Protein Expr. 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