{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T10:37:05Z","timestamp":1774435025203,"version":"3.50.1"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2020,8,6]],"date-time":"2020-08-06T00:00:00Z","timestamp":1596672000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Grants-in-Aid for Scientific Research","award":["17K07336"],"award-info":[{"award-number":["17K07336"]}]},{"name":"Grants-in-Aid for Scientific Research","award":["19K07064"],"award-info":[{"award-number":["19K07064"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,12,26]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Facilitative glucose transporters (GLUTs) play crucial roles in glucose utilization and homeostasis. GLUT12 was initially isolated as a novel GLUT4-like transporter involved in insulin-dependent glucose transport. However, tissue distribution and biochemical properties of GLUT12 are not well understood. In this study, we investigated the basic kinetic properties and tissue distribution of GLUT12. Human GLUT12 and GLUT1 were overexpressed and purified using Ni-NTA column chromatography. Reconstituted proteoliposomes showed time-dependent d-glucose transport activity, which was inhibited by phloretin and dehydroascorbate. Dose dependence of glucose transport revealed a KM and Vmax values of 6.4\u2009mM and 1.2\u2009\u03bcmol\/mg\/min, respectively, indicating that GLUT12 is a high-affinity type GLUT. Glucose transport by GLUT12 was inhibited by ATP and glucose-1-phosphate, glucose-6-phosphate and disaccharides (properties similar to those of GLUT1). Indirect immunohistochemistry revealed the distribution of mouse GLUT12 in the apical region of distal tubules and collecting ducts in the kidney and epithelial cells of the jejunum. In addition to these cells, GLUT12 was present in chromaffin cells in the adrenal medulla, the anterior pituitary lobe, as well as the thyroid and pyloric glands. These tissue distributions suggest a unique function of GLUT12, besides that of an insulin-dependent glucose transport.<\/jats:p>","DOI":"10.1093\/jb\/mvaa090","type":"journal-article","created":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T19:23:49Z","timestamp":1596569029000},"page":"611-620","source":"Crossref","is-referenced-by-count":11,"title":["Functional characterization and tissue localization of the facilitative glucose transporter GLUT12"],"prefix":"10.1093","volume":"168","author":[{"given":"Shunsuke","family":"Matsuo","sequence":"first","affiliation":[{"name":"Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miki","family":"Hiasa","sequence":"additional","affiliation":[{"name":"Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Omote","sequence":"additional","affiliation":[{"name":"Department of Membrane Biochemistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8530, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,8,6]]},"reference":[{"key":"2020122606295021500_mvaa090-B1","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1002\/iub.315","article-title":"The protein family of glucose transport facilitators: it's not only about glucose after all","volume":"62","author":"Augustin","year":"2010","journal-title":"IUBMB Life"},{"key":"2020122606295021500_mvaa090-B2","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.mam.2012.07.001","article-title":"The SLC2 (GLUT) family of membrane transporters","volume":"34","author":"Mueckler","year":"2013","journal-title":"Mol. 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