{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T15:43:02Z","timestamp":1780760582898,"version":"3.54.1"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2021,4,9]],"date-time":"2021-04-09T00:00:00Z","timestamp":1617926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,10,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Diabetic cardiomyopathy (DCM) is a common cardiovascular disease. A declined miR-20a-5p was observed in hearts of diabetic mice, while its effect on DCM remains unknown. Herein, we established streptozotocin-induced DCM rat model and high glucose-stimulated H9C2 model of DCM. Then they were treated with adenovirus expressing miR-20a-5p to explore the function of miR-20a-5p. Insulin tolerance test and intraperitoneal glucose tolerance test assay revealed that miR-20a-5p reduced blood glucose level. Besides, miR-20a-5p improved cardiac dysfunction reflected by reduced heart weight\/body weight and left ventricular diastolic pressure, and increased left ventricular systolic pressure and \u00b1LV dp\/dt max. MiR-20a-5p prevented cardiomyocyte apoptosis, along with the upregulated c-caspase-3, bax and downregulated bcl-2. Moreover, miR-20a-5p alleviated cardiac hypertrophy as the parameters of atrial natriuretic peptide, B-type natriuretic peptide and MyHC-\u03b2 decreased. Also, miR-20a-5p attenuated the cardiac fibrosis demonstrated by decreased transforming growth factor-\u03b21, collagen I levels and the inflammatory response manifested by reduced interleukin-6, tumour necrosis factor-\u03b1 and IL-1\u03b2 production. Furthermore, miR-20a-5p prevented Jun NH2-terminal kinase (JNK) phosphorylation and nuclear factor-\u03baB (NF-\u03baB) p65nuclear translocation. Similarly, the effects of miR-20a-5p on DCM were confirmed in our in vitro experiments. Additionally, ROCK2 is a possible target gene of miR-20a-5p. ROCK2 overexpression reversed the protective effect of miR-20a-5p on DCM. Overall, miR-20a-5p may effectively ameliorate DCM through improving cardiac metabolism, and subsequently inhibiting inflammation, apoptosis, hypertrophy, fibrosis and JNK\/NF-\u03baB pathway via modulating ROCK2.<\/jats:p>","DOI":"10.1093\/jb\/mvab047","type":"journal-article","created":{"date-parts":[[2021,4,8]],"date-time":"2021-04-08T11:12:57Z","timestamp":1617880377000},"page":"349-362","source":"Crossref","is-referenced-by-count":33,"title":["MiR-20a-5p overexpression prevented diabetic cardiomyopathy via inhibition of cardiomyocyte apoptosis, hypertrophy, fibrosis and JNK\/NF-\u03baB signalling pathway"],"prefix":"10.1093","volume":"170","author":[{"given":"Xiaoyu","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Cardiology, The Second Hospital of Hebei Medical University , No. 215, Heping West Road, Shijiazhuang 050000, China"},{"name":"The Third Department of Cardiology, Cangzhou Central Hospital , No.16, Xinhua West Road, Cangzhou 061000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingyan","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The Second Hospital of Hebei Medical University , No. 215, Heping West Road, Shijiazhuang 050000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Third Department of Cardiology, Cangzhou Central Hospital , No.16, Xinhua West Road, Cangzhou 061000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bocong","family":"Ma","sequence":"additional","affiliation":[{"name":"The Third Department of Cardiology, Cangzhou Central Hospital , No.16, Xinhua West Road, Cangzhou 061000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2027-9294","authenticated-orcid":false,"given":"Yongjun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The Second Hospital of Hebei Medical University , No. 215, Heping West Road, Shijiazhuang 050000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,4,9]]},"reference":[{"key":"2022100311590268500_mvab047-B1","doi-asserted-by":"crossref","first-page":"1640","DOI":"10.1016\/j.biopha.2018.10.026","article-title":"CAPE-pNO(2) attenuates diabetic cardiomyopathy through the NOX4\/NF-\u03baB pathway in STZ-induced diabetic mice","volume":"108","author":"Fan","year":"2018","journal-title":"Biomed. 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