{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T04:20:08Z","timestamp":1685593208199},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T00:00:00Z","timestamp":1670371200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,3]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>Mechanistic target of rapamycin complex 1 (mTORC1) is a serine\u2013threonine kinase that is activated by extracellular signals, such as nutrients and growth factors. It plays a key role in the control of various biological processes, such as protein synthesis and energy metabolism by mediating or regulating the phosphorylation of multiple target molecules, some of which remain to be identified. We have here reanalysed a large-scale phosphoproteomics data set for mTORC1 target molecules and identified pre\u2013B cell leukemia transcription factor 2 (PBX2) as such a novel target that is dephosphorylated downstream of mTORC1. We confirmed that PBX2, but not other members of the PBX family, is dephosphorylated in an mTORC1 activity\u2013dependent manner. Furthermore, pharmacological and gene knockdown experiments revealed that glycogen synthase kinase 3 (GSK3) and protein phosphatase 1 (PP1) are responsible for the phosphorylation and dephosphorylation of PBX2, respectively. Our results thus suggest that the balance between the antagonistic actions of GSK3 and PP1 determines the phosphorylation status of PBX2 and its regulation by mTORC1.<\/jats:p>","DOI":"10.1093\/jb\/mvac094","type":"journal-article","created":{"date-parts":[[2022,12,3]],"date-time":"2022-12-03T14:07:05Z","timestamp":1670076425000},"page":"129-138","source":"Crossref","is-referenced-by-count":0,"title":["Phosphorylation of PBX2, a novel downstream target of mTORC1, is determined by GSK3 and PP1"],"prefix":"10.1093","volume":"173","author":[{"given":"Reona","family":"Wada","sequence":"first","affiliation":[{"name":"Medical Institute of Bioregulation Department of Molecular and Cellular Biology, , Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shun","family":"Fujinuma","sequence":"additional","affiliation":[{"name":"Medical Institute of Bioregulation Department of Molecular and Cellular Biology, , Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirokazu","family":"Nakatsumi","sequence":"additional","affiliation":[{"name":"Medical Institute of Bioregulation Department of Molecular and Cellular Biology, , Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaki","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Medical Institute of Bioregulation Department of Molecular and Cellular Biology, , Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan"},{"name":"Graduate School of Medical and Dental Sciences Department of Omics and Systems Biology, , Niigata University, 757 Ichibancho, Asahimachi-dori, Chuo-ku, Niigata City, Niigata 951-8510, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiichi I","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Medical Institute of Bioregulation Department of Molecular and Cellular Biology, , Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,12,7]]},"reference":[{"key":"2023053106413714400_ref1","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1038\/nrm3757","article-title":"Making new contacts: the mTOR network in metabolism and signalling crosstalk","volume":"15","author":"Shimobayashi","year":"2014","journal-title":"Nat. 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