{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T22:41:53Z","timestamp":1787611313414,"version":"build-2736575974"},"reference-count":53,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2023,6,5]],"date-time":"2023-06-05T00:00:00Z","timestamp":1685923200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,7,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Protein modification by glycosylphosphatidylinositol (GPI) takes place in the endoplasmic reticulum (ER). GPI-anchored proteins (GPI-APs) formed in the ER are transported to the cell surface through the Golgi apparatus. During transport, the GPI-anchor structure is processed. In most cells, an acyl chain modified to the inositol of GPI is removed by a GPI-inositol deacylase, PGAP1, in the ER. Inositol-deacylated GPI-APs become sensitive to bacterial phosphatidylinositol-specific phospholipase C (PI-PLC). We previously reported that GPI-APs are partially resistant to PI-PLC when PGAP1 activity is weakened by the deletion of selenoprotein T (SELT) or cleft lip and palate transmembrane protein 1 (CLPTM1). In this study, we found that the loss of TMEM41B, an ER-localized lipid scramblase, restored PI-PLC sensitivity of GPI-APs in SELT-knockout (KO) and CLPTM1-KO cells. In TMEM41B-KO cells, the transport of GPI-APs as well as transmembrane proteins from the ER to the Golgi was delayed. Furthermore, the turnover of PGAP1, which is mediated by ER-associated degradation, was slowed in TMEM41B-KO cells. Taken together, these findings indicate that inhibition of TMEM41B-dependent lipid scrambling promotes GPI-AP processing in the ER through PGAP1 stabilization and slowed protein trafficking.<\/jats:p>","DOI":"10.1093\/jb\/mvad041","type":"journal-article","created":{"date-parts":[[2023,6,9]],"date-time":"2023-06-09T12:16:40Z","timestamp":1686313000000},"page":"109-123","source":"Crossref","is-referenced-by-count":8,"title":["A lipid scramblase TMEM41B is involved in the processing and transport of GPI-anchored proteins"],"prefix":"10.1093","volume":"174","author":[{"given":"Shu-Ya","family":"Cao","sequence":"first","affiliation":[{"name":"Jiangnan University Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, , 1800 Lihu Avenue, Wuxi, Jiangsu 214122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Shi","family":"Liu","sequence":"additional","affiliation":[{"name":"Jiangnan University Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, , 1800 Lihu Avenue, Wuxi, Jiangsu 214122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Dong","family":"Gao","sequence":"additional","affiliation":[{"name":"Jiangnan University Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, , 1800 Lihu Avenue, Wuxi, Jiangsu 214122, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taroh","family":"Kinoshita","sequence":"additional","affiliation":[{"name":"Osaka University Research Institute for Microbial Diseases, , 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan"},{"name":"Osaka University WPI Immunology Frontier Research Center, , 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0344-2408","authenticated-orcid":false,"given":"Morihisa","family":"Fujita","sequence":"additional","affiliation":[{"name":"Jiangnan University Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, , 1800 Lihu Avenue, Wuxi, Jiangsu 214122, China"},{"name":"Gifu University Institute for Glyco-core Research (iGCORE), , 1-1 Yanagido, Gifu 501-1193, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,6,5]]},"reference":[{"key":"2024030619355037600_ref1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-4939-7262-3_1","article-title":"An overview of protein secretion in yeast and animal cells","volume":"1662","author":"Guo","year":"2017","journal-title":"Methods Mol. 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