{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T12:53:30Z","timestamp":1792155210278,"version":"4.4.0"},"reference-count":57,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2024,1,2]],"date-time":"2024-01-02T00:00:00Z","timestamp":1704153600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Grants-in-Aid for Scientific Research","doi-asserted-by":"publisher","award":["23K07165"],"award-info":[{"award-number":["23K07165"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Grants-in-Aid for Scientific Research","doi-asserted-by":"publisher","award":["18K07241"],"award-info":[{"award-number":["18K07241"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Lymphedema has become a global health issue following the growing number of cancer surgeries. Curative or supportive therapeutics have long been awaited for this refractory condition. Transcription factor GATA2 is crucial in lymphatic development and maintenance, as GATA2 haploinsufficient disease often manifests as lymphedema. We recently demonstrated that Gata2 heterozygous deficient mice displayed delayed lymphatic recanalization upon lymph node resection. However, whether GATA2 contributes to lymphatic regeneration by functioning in the damaged lymph vessels' microenvironment remains explored. In this study, our integrated analysis demonstrated that dermal collagen fibers were more densely accumulated in the Gata2 heterozygous deficient mice. The collagen metabolism-related transcriptome was perturbed, and collagen matrix contractile activity was aberrantly increased in Gata2 heterozygous embryonic fibroblasts. Notably, soluble collagen placement ameliorated delayed lymphatic recanalization, presumably by modulating the stiffness of the extracellular matrix around the resection site of Gata2 heterozygous deficient mice. Our results provide valuable insights into mechanisms underlying GATA2-haploinsufficiency-mediated lymphedema and shed light on potential therapeutic avenues for this intractable disease.<\/jats:p>","DOI":"10.1093\/jb\/mvad122","type":"journal-article","created":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T11:10:16Z","timestamp":1704280216000},"page":"551-560","source":"Crossref","is-referenced-by-count":6,"title":["Perturbed collagen metabolism underlies lymphatic recanalization failure in <i>Gata2<\/i> heterozygous deficient mice"],"prefix":"10.1093","volume":"175","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6222-0613","authenticated-orcid":false,"given":"Tomomi","family":"Watanabe-Asaka","sequence":"first","affiliation":[{"name":"Division of Physiology, Tohoku Medical and Pharmaceutical University , School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moyuru","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Division of Physiology, Tohoku Medical and Pharmaceutical University , School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuya","family":"Harada","sequence":"additional","affiliation":[{"name":"Division of Physiology, Tohoku Medical and Pharmaceutical University , School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Uemura","sequence":"additional","affiliation":[{"name":"Division of Medical Biochemistry, Tohoku Medical and Pharmaceutical University , School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Takai","sequence":"additional","affiliation":[{"name":"Division of Medical Biochemistry, Tohoku Medical and Pharmaceutical University , School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiro","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Tohoku Medical and Pharmaceutical University, School of Medicine Division of Pathology, 983-8536 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Moriguchi","sequence":"additional","affiliation":[{"name":"Division of Medical Biochemistry, Tohoku Medical and Pharmaceutical University , School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2011-2603","authenticated-orcid":false,"given":"Yoshiko","family":"Kawai","sequence":"additional","affiliation":[{"name":"Division of Physiology, Tohoku Medical and Pharmaceutical University , School of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,1,2]]},"reference":[{"key":"2024050904162592300_ref1","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1016\/j.cell.2010.01.045","article-title":"Lymphangiogenesis: molecular mechanisms and future promise","volume":"140","author":"Tammela","year":"2010","journal-title":"Cell"},{"key":"2024050904162592300_ref2","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1196\/annals.1413.014","article-title":"Estimating the population burden of lymphedema","volume":"1131","author":"Rockson","year":"2008","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"2024050904162592300_ref3","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s13561-018-0194-6","article-title":"Worldwide assessment of healthcare personnel dealing with lymphoedema","volume":"8","author":"Schulze","year":"2018","journal-title":"Heal. Econ. Rev."},{"key":"2024050904162592300_ref4","doi-asserted-by":"crossref","first-page":"898","DOI":"10.1172\/JCI71614","article-title":"Genetics of lymphatic anomalies","volume":"124","author":"Brouillard","year":"2014","journal-title":"J. Clin. Invest."},{"key":"2024050904162592300_ref5","doi-asserted-by":"crossref","first-page":"1650","DOI":"10.1101\/gad.4.10.1650","article-title":"Activity and tissue-specific expression of the transcription factor NF-E1 multigene family","volume":"4","author":"Yamamoto","year":"1990","journal-title":"Genes Dev."},{"key":"2024050904162592300_ref6","first-page":"4011","article-title":"DNA-binding specificities of the GATA transcription factor family","volume":"13","author":"Ko","year":"1993","journal-title":"Mol. Cell. Biol."},{"key":"2024050904162592300_ref7","doi-asserted-by":"crossref","first-page":"2979","DOI":"10.1172\/JCI78888","article-title":"GATA2 is required for lymphatic vessel valve development and maintenance","volume":"125","author":"Kazenwadel","year":"2015","journal-title":"J. Clin. Invest."},{"key":"2024050904162592300_ref8","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1038\/ng.923","article-title":"Mutations in GATA2 cause primary lymphedema associated with a predisposition to acute myeloid leukemia (Emberger syndrome)","volume":"43","author":"Ostergaard","year":"2011","journal-title":"Nat. Genet."},{"key":"2024050904162592300_ref9","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1111\/bjd.15548","article-title":"Skin manifestations among GATA2-deficient patients","volume":"178","author":"Polat","year":"2018","journal-title":"Br. J. Dermatol."},{"key":"2024050904162592300_ref10","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.ijporl.2018.02.014","article-title":"Emberger syndrome: A rare association with hearing loss","volume":"108","author":"Zawawi","year":"2018","journal-title":"Int. J. Pediatr. Otorhinolaryngol."},{"key":"2024050904162592300_ref11","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1111\/bjh.16941","article-title":"GATA2 deficiency in a young man with lymphoedema","volume":"191","author":"Mishra","year":"2020","journal-title":"Br. J. Haematol."},{"key":"2024050904162592300_ref12","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1002\/humu.22223","article-title":"FLT4\/VEGFR3 and Milroy disease: novel mutations, a review of published variants and database update","volume":"34","author":"Gordon","year":"2013","journal-title":"Hum. Mutat."},{"key":"2024050904162592300_ref13","first-page":"478","article-title":"Genetic predisposition in cancer-related lymphedema: A systematic review","volume":"20","author":"Kapellas","year":"2022","journal-title":"Lymphat. Res. Biol."},{"key":"2024050904162592300_ref14","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1080\/10739680600745810","article-title":"Recanalization of the collecting lymphatics in rabbit hind leg","volume":"13","author":"Ikomi","year":"2006","journal-title":"Microcirculation (New York, N.Y. : 1994)"},{"key":"2024050904162592300_ref15","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1080\/10739680701815538","article-title":"Critical roles of VEGF-C-VEGF receptor 3 in reconnection of the collecting lymph vessels in mice","volume":"15","author":"Ikomi","year":"2008","journal-title":"Microcirculation (New York, N.Y. : 1994)"},{"key":"2024050904162592300_ref16","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1111\/gtc.12852","article-title":"GATA2 participates in the recanalization of lymphatic vessels after surgical lymph node extirpation","volume":"26","author":"Watanabe-Asaka","year":"2021","journal-title":"Genes to cells: devoted to molecular and cellular mechanisms"},{"key":"2024050904162592300_ref17","doi-asserted-by":"crossref","first-page":"888","DOI":"10.1172\/JCI71609","article-title":"Development of the mammalian lymphatic vasculature","volume":"124","author":"Yang","year":"2014","journal-title":"J. Clin. Invest."},{"key":"2024050904162592300_ref18","doi-asserted-by":"crossref","first-page":"1511","DOI":"10.1038\/s41467-018-03959-6","article-title":"Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program","volume":"9","author":"Frye","year":"2018","journal-title":"Nat. Commun."},{"key":"2024050904162592300_ref19","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a004978","article-title":"The collagen family","volume":"3","author":"Ricard-Blum","year":"2011","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"2024050904162592300_ref20","doi-asserted-by":"crossref","first-page":"407","DOI":"10.3390\/ijms11020407","article-title":"The fibrillar collagen family","volume":"11","author":"Exposito","year":"2010","journal-title":"Int. J. Mol. Sci."},{"key":"2024050904162592300_ref21","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1016\/j.patbio.2004.12.033","article-title":"Lysyl oxidase in development, aging and pathologies of the skin","volume":"53","author":"Szauter","year":"2005","journal-title":"Pathologie-biologie"},{"key":"2024050904162592300_ref22","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1042\/EBC20180050","article-title":"Lysyl oxidases: from enzyme activity to extracellular matrix cross-links","volume":"63","author":"Vallet","year":"2019","journal-title":"Essays Biochem."},{"key":"2024050904162592300_ref23","doi-asserted-by":"crossref","first-page":"868","DOI":"10.3390\/ijms17060868","article-title":"Role of matrix metalloproteinases in Photoaging and Photocarcinogenesis","volume":"17","author":"Pittayapruek","year":"2016","journal-title":"Int. J. Mol. Sci."},{"key":"2024050904162592300_ref24","doi-asserted-by":"crossref","first-page":"9739","DOI":"10.3390\/ijms21249739","article-title":"The roles of matrix metalloproteinases and their inhibitors in human diseases","volume":"21","author":"Cabral-Pacheco","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"2024050904162592300_ref25","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1016\/j.bbrc.2008.02.050","article-title":"Plasminogen N-terminal activation peptide modulates the activity of angiostatin-related peptides on endothelial cell proliferation and migration","volume":"369","author":"Hayashi","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2024050904162592300_ref26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11010-008-9934-y","article-title":"Impact of plasminogen on an in vitro wound healing model based on a perfusion cell culture system","volume":"322","author":"Hayashi","year":"2009","journal-title":"Mol. Cell. Biochem."},{"key":"2024050904162592300_ref27","doi-asserted-by":"crossref","first-page":"39027","DOI":"10.1074\/jbc.M006700200","article-title":"Effect of collagen turnover on the accumulation of advanced glycation end products","volume":"275","author":"Verzijl","year":"2000","journal-title":"J. Biol. Chem."},{"key":"2024050904162592300_ref28","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1038\/nrm3496","article-title":"Protein homeostasis: live long, won't prosper","volume":"14","author":"Toyama","year":"2013","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2024050904162592300_ref29","first-page":"87","article-title":"The role of the extracellular matrix (ECM) in wound healing: A review","volume":"7","author":"Diller","year":"2022","journal-title":"Biomimetics (Basel, Switzerland)"},{"key":"2024050904162592300_ref30","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1182\/blood-2002-12-3809","article-title":"Expression and domain-specific function of GATA-2 during differentiation of the hematopoietic precursor cells in midgestation mouse embryos","volume":"102","author":"Minegishi","year":"2003","journal-title":"Blood"},{"key":"2024050904162592300_ref31","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1538\/expanim.60.481","article-title":"Effect of three types of mixed anesthetic agents alternate to ketamine in mice","volume":"60","author":"Kawai","year":"2011","journal-title":"Exp. Anim."},{"key":"2024050904162592300_ref32","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1097\/BRS.0000000000000795","article-title":"Chondrogenic and fibrotic process in the ligamentum flavum of patients with lumbar spinal canal stenosis","volume":"40","author":"Yabe","year":"2015","journal-title":"Spine"},{"key":"2024050904162592300_ref33","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1002\/jor.23130","article-title":"Decreased elastic fibers and increased proteoglycans in the ligamentum flavum of patients with lumbar spinal canal stenosis","volume":"34","author":"Yabe","year":"2016","journal-title":"Journal of orthopaedic research: official publication of the Orthopaedic Research Society"},{"key":"2024050904162592300_ref34","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1111\/j.1349-7006.2012.02289.x","article-title":"Pivotal roles of shear stress in the microenvironmental changes that occur within sentinel lymph nodes","volume":"103","author":"Kawai","year":"2012","journal-title":"Cancer Sci."},{"key":"2024050904162592300_ref35","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1007\/BF01002772","article-title":"Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections","volume":"11","author":"Junqueira","year":"1979","journal-title":"Histochem. J."},{"key":"2024050904162592300_ref36","first-page":"651","article-title":"Implication of collagen type I-induced membrane-type 1-matrix metalloproteinase expression and matrix metalloproteinase-2 activation in the metastatic progression of breast carcinoma","volume":"76","author":"Gilles","year":"1997","journal-title":"Laboratory investigation; a journal of technical methods and pathology"},{"key":"2024050904162592300_ref37","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1146\/annurev-cellbio-092910-154013","article-title":"Transmembrane collagen receptors","volume":"27","author":"Leitinger","year":"2011","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"2024050904162592300_ref38","doi-asserted-by":"crossref","first-page":"12","DOI":"10.3389\/fphy.2017.00012","article-title":"Collagen type I as a ligand for receptor-mediated signaling","volume":"5","author":"Boraschi-Diaz","year":"2017","journal-title":"Front. Phys."},{"key":"2024050904162592300_ref39","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1038\/9560","article-title":"New delivery system for plasmid DNA in vivo using atelocollagen as a carrier material: the Minipellet","volume":"5","author":"Ochiya","year":"1999","journal-title":"Nat. Med."},{"key":"2024050904162592300_ref40","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1002\/jcp.22783","article-title":"PAI-1 in tissue fibrosis","volume":"227","author":"Ghosh","year":"2012","journal-title":"J. Cell. Physiol."},{"key":"2024050904162592300_ref41","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1038\/368419a0","article-title":"Physiological consequences of loss of plasminogen activator gene function in mice","volume":"368","author":"Carmeliet","year":"1994","journal-title":"Nature"},{"key":"2024050904162592300_ref42","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/(SICI)1097-0215(19970703)72:1<1::AID-IJC1>3.0.CO;2-Z","article-title":"The urokinase-type plasminogen activator system in cancer metastasis: a review","volume":"72","author":"Andreasen","year":"1997","journal-title":"Int. J. Cancer"},{"key":"2024050904162592300_ref43","doi-asserted-by":"crossref","DOI":"10.1155\/2013\/230805","article-title":"Extracellular matrix degradation and tissue remodeling in periprosthetic loosening and osteolysis: focus on matrix metalloproteinases, their endogenous tissue inhibitors, and the proteasome","volume":"2013","author":"Syggelos","year":"2013","journal-title":"Biomed. Res. Int."},{"key":"2024050904162592300_ref44","first-page":"635","article-title":"Whole cell mechanics of contractile fibroblasts: relations between effective cellular and extracellular matrix moduli","volume":"368","author":"Marquez","year":"2010","journal-title":"Philosophical transactions. Series A, Mathematical, physical, and engineering sciences"},{"key":"2024050904162592300_ref45","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1073\/pnas.76.3.1274","article-title":"Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro","volume":"76","author":"Bell","year":"1979","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2024050904162592300_ref46","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/S0962-8924(03)00057-6","article-title":"Fibroblast biology in three-dimensional collagen matrices","volume":"13","author":"Grinnell","year":"2003","journal-title":"Trends Cell Biol."},{"key":"2024050904162592300_ref47","doi-asserted-by":"crossref","DOI":"10.1016\/j.ejcb.2022.151253","article-title":"Collagen gel contraction assays: from modelling wound healing to quantifying cellular interactions with three-dimensional extracellular matrices","volume":"101","author":"Zhang","year":"2022","journal-title":"Eur. J. Cell Biol."},{"key":"2024050904162592300_ref48","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0271776","article-title":"Collagen cross-links scale with passive stiffness in dystrophic mouse muscles, but are not altered with administration of a lysyl oxidase inhibitor","volume":"17","author":"Brashear","year":"2022","journal-title":"PLoS One"},{"key":"2024050904162592300_ref49","doi-asserted-by":"crossref","DOI":"10.1016\/j.acthis.2020.151634","article-title":"Comparison of similar cells: mesenchymal stromal cells and fibroblasts","volume":"122","author":"Ugurlu","year":"2020","journal-title":"Acta Histochem."},{"key":"2024050904162592300_ref50","doi-asserted-by":"crossref","first-page":"1686","DOI":"10.3324\/haematol.2014.105692","article-title":"GATA2 regulates differentiation of bone marrow-derived mesenchymal stem cells","volume":"99","author":"Kamata","year":"2014","journal-title":"Haematologica"},{"key":"2024050904162592300_ref51","doi-asserted-by":"crossref","first-page":"1028926","DOI":"10.3389\/fphar.2022.1028926","article-title":"Doxycycline for the treatment of breast cancer-related lymphedema","volume":"13","author":"Brown","year":"2022","journal-title":"Front. Pharmacol."},{"key":"2024050904162592300_ref52","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1089\/lrb.2020.0012","article-title":"Utilization of vascular endothelial growth factor-C156S in therapeutic Lymphangiogenesis: A systematic review","volume":"20","author":"Forte","year":"2022","journal-title":"Lymphat. Res. Biol."},{"key":"2024050904162592300_ref53","first-page":"14","article-title":"Lymphoedema: modern diagnostic and therapeutic aspects","volume":"18","author":"Campisi","year":"1999","journal-title":"International angiology: a journal of the International Union of Angiology"},{"key":"2024050904162592300_ref54","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1146\/annurev.pathmechdis.3.121806.151515","article-title":"Molecular biology and pathology of lymphangiogenesis","volume":"3","author":"Karpanen","year":"2008","journal-title":"Annu. Rev. Pathol."},{"key":"2024050904162592300_ref55","doi-asserted-by":"crossref","first-page":"7774","DOI":"10.3390\/ijms24097774","article-title":"Therapeutic Lymphangiogenesis is a promising strategy for secondary lymphedema","volume":"24","author":"Shimizu","year":"2023","journal-title":"Int. J. Mol. Sci."},{"key":"2024050904162592300_ref56","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.3390\/polym15041020","article-title":"An update on the clinical efficacy and safety of collagen Injectables for aesthetic and regenerative medicine applications","volume":"15","author":"Salvatore","year":"2023","journal-title":"Polymers"},{"key":"2024050904162592300_ref57","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gng154","article-title":"A PCR primer bank for quantitative gene expression analysis","volume":"31","author":"Wang","year":"2003","journal-title":"Nucleic Acids Res."}],"container-title":["The Journal of Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jb\/advance-article-pdf\/doi\/10.1093\/jb\/mvad122\/56751971\/mvad122.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jb\/article-pdf\/175\/5\/551\/57458065\/mvad122.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jb\/article-pdf\/175\/5\/551\/57458065\/mvad122.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T04:18:10Z","timestamp":1715228290000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jb\/article\/175\/5\/551\/7505281"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,2]]},"references-count":57,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2024,1,2]]},"published-print":{"date-parts":[[2024,4,29]]}},"URL":"https:\/\/doi.org\/10.1093\/jb\/mvad122","relation":{},"ISSN":["0021-924X","1756-2651"],"issn-type":[{"value":"0021-924X","type":"print"},{"value":"1756-2651","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024,5]]},"published":{"date-parts":[[2024,1,2]]}}}