{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T22:42:17Z","timestamp":1785451337351,"version":"3.56.0"},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["#21K09816"],"award-info":[{"award-number":["#21K09816"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["#21K15628"],"award-info":[{"award-number":["#21K15628"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,5,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Vascular calcification, a major risk factor for cardiovascular events, is associated with a poor prognosis in chronic kidney disease (CKD) patients. This process is often associated with the transformation of vascular smooth muscle cells (VSMCs) into cells with osteoblast-like characteristics. Damage-associated molecular patterns (DAMPs), such as extracellular histones released from damaged or dying cells, are suspected to accumulate at calcification sites. To investigate the potential involvement of DAMPs in vascular calcification, we assessed the impact of externally added histones (extracellular histones) on calcium and inorganic phosphate-induced calcification in mouse VSMCs. Our study found that extracellular histones intensified calcification. We also observed that the histones decreased the expression of VSMC marker genes while simultaneously increasing the expression of osteoblast marker genes. Additionally, histones treated with DNase I, which degrades dsDNA, attenuated this calcification, compared with the non-treated histones, suggesting a potential involvement of dsDNA in this process. Elevated levels of dsDNA were also detected in the serum of CKD model mice, underlining its potential role in vascular calcification in CKD. Our findings suggest that extracellular histones could play a pivotal role in the vascular calcification observed in CKD.<\/jats:p>","DOI":"10.1093\/jb\/mvae011","type":"journal-article","created":{"date-parts":[[2024,1,30]],"date-time":"2024-01-30T23:56:44Z","timestamp":1706659004000},"page":"643-648","source":"Crossref","is-referenced-by-count":5,"title":["Extracellular histones promote calcium phosphate-dependent calcification in mouse vascular smooth muscle cells"],"prefix":"10.1093","volume":"175","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2737-5326","authenticated-orcid":false,"given":"Tomonori","family":"Hoshino","sequence":"first","affiliation":[{"name":"Hiroshima University Graduate School of Biomedical and Health Sciences Department of Calcified Tissue Biology, , 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan"},{"name":"Massachusetts General Hospital and Harvard Medical School Neuroprotection Research Laboratories, Departments of Neurology and Radiology, , 149 Thirteenth Street, Charlestown, MA 02129, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davood","family":"Kharaghani","sequence":"additional","affiliation":[{"name":"Hiroshima University Graduate School of Biomedical and Health Sciences Department of Calcified Tissue Biology, , 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan"},{"name":"The State University of New Jersey Nanoscience and Advanced Materials Center, Environmental and Occupational Health Sciences Institute (EOHSI) and School of Public Health, Rutgers-New Brunswick, , 170 Frelinghuysen Road, Piscataway, NJ 08854, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shohei","family":"Kohno","sequence":"additional","affiliation":[{"name":"Hiroshima University Graduate School of Biomedical and Health Sciences Department of Calcified Tissue Biology, , 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan"},{"name":"Hiroshima University Graduate School of Biomedical and Health Sciences Department of Maxillofacial Anatomy and Neuroscience, , 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,2,1]]},"reference":[{"key":"2024060710311047600_ref1","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1161\/CIRCRESAHA.110.234914","article-title":"Arterial calcification in chronic kidney disease: key roles for calcium and phosphate","volume":"109","author":"Shanahan","year":"2011","journal-title":"Circ. 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