{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T18:09:19Z","timestamp":1791828559478,"version":"4.3.4"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,3,18]],"date-time":"2024-03-18T00:00:00Z","timestamp":1710720000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,7,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Glycogen debranching enzyme is a single polypeptide with distinct catalytic sites for 4-\u03b1-glucanotransferase and amylo-\u03b1-1,6-glucosidase. To allow phosphorylase to degrade the inner tiers of highly branched glycogen, 4-\u03b1-glucanotransferase converts the phosphorylase-limit biantennary branch G-G-G-G-(G-G-G-G\u2194)G-G- (G: d-glucose, hyphens: \u03b1-1,4-linkages; double-headed arrow: \u03b1-1,6-linkage) into the G-G-G-G-(G\u2194)G-G- residue, which is then subjected to amylo-\u03b1-1,6-glucosidase to release the remaining G\u2194 residue. However, while the essential side-chain structure of the 4-\u03b1-glucanotransferase donor substrate has been determined to be the G-G-G-G\u2194 residue (Watanabe, Y., et\u00a0al. (2008) J. Biochem.143, 435\u2013440), its essential main-chain structure remains to be investigated. In this study, we probed the 4-\u03b1-glucanotransferase donor-binding region using novel fluorogenic dextrins Gm-(G4\u2194)G-Gn-F (F: 1-deoxy-1-[(2-pyridyl)amino]-d-glucitol) and maltohexaose (G6) as the donor and acceptor substrates, respectively. 4-\u03b1-Glucanotransferase exhibited maximum activity towards G4-(G4\u2194)G-F and G4-(G4\u2194)G-G-F, indicating that recognition of the G4-(G4\u2194)G-moiety was essential for full enzyme function. Notably, when the 4-\u03b1-glucanotransferase activity towards G4-(G4\u2194)G-G-F was taken as unity, those towards nonbranching dextrins were\u2009&amp;lt;\u20090.001. This indicated that the disproportionation activities towards maltooligosaccharides (Gm) are abnormal behaviours of 4-\u03b1-glucanotransferase. Notably, however, these activities have been traditionally measured to identify the 4-\u03b1-glucanotransferase mutations causing glycogen storage disease type III. This study provides a basis for more accurate identification.<\/jats:p>","DOI":"10.1093\/jb\/mvae030","type":"journal-article","created":{"date-parts":[[2024,3,18]],"date-time":"2024-03-18T22:11:48Z","timestamp":1710799908000},"page":"109-117","source":"Crossref","is-referenced-by-count":2,"title":["Essential dextrin structure as donor substrate for 4-\u03b1-glucanotransferase in glycogen debranching enzyme"],"prefix":"10.1093","volume":"176","author":[{"given":"Rentaro","family":"Uno","sequence":"first","affiliation":[{"name":"Graduate School of Science, Osaka Prefecture University Department of Chemistry, , Gakuen-cho 1-1, Naka-ku, Sakai, Osaka 599-8531, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8827-7412","authenticated-orcid":false,"given":"Yasushi","family":"Makino","sequence":"additional","affiliation":[{"name":"Graduate School of Science, Osaka Prefecture University Department of Chemistry, , Gakuen-cho 1-1, Naka-ku, Sakai, Osaka 599-8531, Japan"},{"name":"Graduate School of Science, Osaka Metropolitan University Department of Chemistry, , Gakuen-cho 1-1, Naka-ku, Sakai, Osaka 599-8531, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3224-4336","authenticated-orcid":false,"given":"Hiroshi","family":"Matsubara","sequence":"additional","affiliation":[{"name":"Graduate School of Science, Osaka Prefecture University Department of Chemistry, , Gakuen-cho 1-1, Naka-ku, Sakai, Osaka 599-8531, Japan"},{"name":"Graduate School of Science, Osaka Metropolitan University Department of Chemistry, , Gakuen-cho 1-1, Naka-ku, Sakai, Osaka 599-8531, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,3,18]]},"reference":[{"key":"2024073106472284900_ref1","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1042\/BJ20111416","article-title":"Glycogen and its metabolism: some new developments and old themes","volume":"441","author":"Roach","year":"2012","journal-title":"Biochem. 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