{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T23:39:26Z","timestamp":1790206766892,"version":"4.1.0"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T00:00:00Z","timestamp":1722297600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science KAKENHI","doi-asserted-by":"crossref","award":["17H06157 and 20K05226"],"award-info":[{"award-number":["17H06157 and 20K05226"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Nucleotide excision repair (NER) is a major DNA repair system and hereditary defects in this system cause critical genetic diseases (e.g. xeroderma pigmentosum, Cockayne syndrome and trichothiodystrophy). Various proteins are involved in the eukaryotic NER system and undergo several post-translational modifications. Damaged DNA-binding protein 2 (DDB2) is a DNA damage recognition factor in the NER pathway. We previously demonstrated that DDB2 was SUMOylated in response to UV irradiation; however, its physiological roles remain unclear. We herein analysed several mutants and showed that the N-terminal tail of DDB2 was the target for SUMOylation; however, this region did not contain a consensus SUMOylation sequence. We found a SUMO-interacting motif (SIM) in the N-terminal tail that facilitated SUMOylation. The ubiquitination of a SUMOylation-deficient DDB2 SIM mutant was decreased, and its retention of chromatin was prolonged. The SIM mutant showed impaired NER, possibly due to a decline in the timely handover of the lesion site to XP complementation group C. These results suggest that the SUMOylation of DDB2 facilitates NER through enhancements in ubiquitination.<\/jats:p>","DOI":"10.1093\/jb\/mvae056","type":"journal-article","created":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T19:42:28Z","timestamp":1722368548000},"page":"325-338","source":"Crossref","is-referenced-by-count":2,"title":["Sequential post-translational modifications regulate damaged DNA-binding protein DDB2 function"],"prefix":"10.1093","volume":"176","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6495-6691","authenticated-orcid":false,"given":"Hidenori","family":"Kaneoka","sequence":"first","affiliation":[{"name":"Nagoya University Department of Biomolecular Engineering, Graduate School of Engineering, , Furo-cho, Chikusa-ku, Nagoya 464-8603 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiko","family":"Arakawa","sequence":"additional","affiliation":[{"name":"Nagoya University Department of Biomolecular Engineering, Graduate School of Engineering, , Furo-cho, Chikusa-ku, Nagoya 464-8603 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusuke","family":"Masuda","sequence":"additional","affiliation":[{"name":"Nagoya University Department of Biomolecular Engineering, Graduate School of Engineering, , Furo-cho, Chikusa-ku, Nagoya 464-8603 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daiki","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Nagoya University Department of Biomolecular Engineering, Graduate School of Engineering, , Furo-cho, Chikusa-ku, Nagoya 464-8603 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kota","family":"Sugimoto","sequence":"additional","affiliation":[{"name":"Nagoya University Department of Biomolecular Engineering, Graduate School of Engineering, , Furo-cho, Chikusa-ku, Nagoya 464-8603 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Risako","family":"Fukata","sequence":"additional","affiliation":[{"name":"Nagoya University Department of Biomolecular Engineering, Graduate School of Engineering, , Furo-cho, Chikusa-ku, Nagoya 464-8603 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maasa","family":"Tsuge-Shoji","sequence":"additional","affiliation":[{"name":"Nagoya University Department of Biomolecular Engineering, Graduate School of Engineering, , Furo-cho, Chikusa-ku, Nagoya 464-8603 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken-ichi","family":"Nishijima","sequence":"additional","affiliation":[{"name":"Nagoya University Department of Biomolecular Engineering, Graduate School of Engineering, , Furo-cho, Chikusa-ku, Nagoya 464-8603 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Iijima","sequence":"additional","affiliation":[{"name":"Nagoya University Department of Biomolecular Engineering, Graduate School of Engineering, , Furo-cho, Chikusa-ku, Nagoya 464-8603 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,7,30]]},"reference":[{"key":"2024103011352491200_ref1","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1016\/S1568-7864(02)00052-6","article-title":"Xeroderma pigmentosum complementation group E and UV-damaged DNA-binding protein","volume":"1","author":"Tang","year":"2002","journal-title":"DNA Repair (Amst)"},{"key":"2024103011352491200_ref2","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1073\/pnas.96.2.424","article-title":"Expression of the p48 xeroderma pigmentosum gene is p53-dependent and is involved in global genomic repair","volume":"96","author":"Hwang","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2024103011352491200_ref3","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1016\/j.dnarep.2005.01.001","article-title":"The UV-damaged DNA binding protein mediates efficient targeting of the nucleotide excision repair complex to UV-induced photo lesions","volume":"4","author":"Moser","year":"2005","journal-title":"DNA Repair (Amst)"},{"key":"2024103011352491200_ref4","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1016\/0092-8674(95)90289-9","article-title":"Mammalian DNA nucleotide excision repair reconstituted with purified protein components","volume":"80","author":"Aboussekhra","year":"1995","journal-title":"Cell"},{"key":"2024103011352491200_ref5","doi-asserted-by":"crossref","first-page":"35309","DOI":"10.1074\/jbc.274.50.35309","article-title":"Cullin 4A associates with the UV-damaged DNA-binding protein DDB","volume":"274","author":"Shiyanov","year":"1999","journal-title":"J. Biol. Chem."},{"key":"2024103011352491200_ref6","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/S0092-8674(03)00316-7","article-title":"The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage","volume":"113","author":"Groisman","year":"2003","journal-title":"Cell"},{"key":"2024103011352491200_ref7","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/j.cell.2005.02.035","article-title":"UV-induced ubiquitylation of XPC protein mediated by UV-DDB-ubiquitin ligase complex","volume":"121","author":"Sugasawa","year":"2005","journal-title":"Cell"},{"key":"2024103011352491200_ref8","doi-asserted-by":"crossref","first-page":"1700","DOI":"10.1093\/nar\/gkv038","article-title":"Functional regulation of the DNA damage-recognition factor DDB2 by ubiquitination and interaction with xeroderma pigmentosum group C protein","volume":"43","author":"Matsumoto","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"2024103011352491200_ref9","doi-asserted-by":"crossref","first-page":"2588","DOI":"10.1073\/pnas.0511160103","article-title":"The DDB1-CUL4ADDB2 ubiquitin ligase is deficient in xeroderma pigmentosum group E and targets histone H2A at UV-damaged DNA sites","volume":"103","author":"Kapetanaki","year":"2006","journal-title":"Proc. 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