{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T04:03:42Z","timestamp":1745899422552,"version":"3.40.4"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T00:00:00Z","timestamp":1737504000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,4,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The uterine endometrium consists of luminal epithelium, glandular epithelium and stromal cells, with uterine glands playing a pivotal role in pregnancy success among mammals. Uterine glands secrete essential factors that regulate embryo development and implantation; however, their cellular biology remains poorly understood. This study presents a refined method for isolating three distinct endometrial cell types with high purity, with a specific emphasis on glandular epithelial (GE) cells. The method combines mechanical dissociation, enzymatic digestion and immunomagnetic separation. The isolated GE cells were maintained in culture and exhibited proliferation in response to steroid hormones. Furthermore, oestrogen responsiveness was abrogated by Estrogen Receptor 1 (Esr1) knockdown mediated by siRNA. Here, we present an efficient and reproducible method for isolating uterine GE cells with high purity, enabling their in vitro maintenance, hormone responsiveness assessment and functional gene knockdown. These findings establish a robust platform for advancing our understanding of uterine gland biology, facilitating detailed investigations into molecular mechanisms underlying glandular function and their critical roles in establishing pregnancy success. Future research could explore the contribution of these isolated cells to endometrial receptivity and embryo implantation.<\/jats:p>","DOI":"10.1093\/jb\/mvaf006","type":"journal-article","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T16:12:53Z","timestamp":1737562373000},"page":"329-337","source":"Crossref","is-referenced-by-count":0,"title":["A refined method for high-purity isolation of uterine glandular epithelial cells in mouse"],"prefix":"10.1093","volume":"177","author":[{"given":"Asmaa M","family":"Youssef","sequence":"first","affiliation":[{"name":"Department of Comparative and Experimental Medicine, Graduate School of Medical Sciences, Nagoya City University , 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, 467-8601 ,","place":["Japan"]},{"name":"Biotechnology Department, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC) , Alsayd Club Street, Dokki, Kafrelsheikh 12619 ,","place":["Egypt"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed M","family":"Moustafa","sequence":"additional","affiliation":[{"name":"Department of Comparative and Experimental Medicine, Graduate School of Medical Sciences, Nagoya City University , 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, 467-8601 ,","place":["Japan"]},{"name":"Zoology Department, Faculty of Science, Al-Azhar University , Assiut Branch, Al Mokhaym Al Daem, Gameat Al Azhar, Cairo Governorate 4434103 ,","place":["Egypt"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Motoharu","family":"Hamada","sequence":"additional","affiliation":[{"name":"Department of Virology, Graduate School of Medical Sciences, Nagoya City University , 1 Kawasumi, Mizuhocho, Mizuho-ku, Nagoya, 467-8601 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayumi","family":"Sugiura-Ogasawara","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Graduate School of Medical Sciences, Nagoya City University , 1 Kawasumi, Mizuhocho, Mizuho-ku, Nagoya, 467-8601 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9988-6993","authenticated-orcid":false,"given":"Hisashi","family":"Oishi","sequence":"additional","affiliation":[{"name":"Department of Comparative and Experimental Medicine, Graduate School of Medical Sciences, Nagoya City University , 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, 467-8601 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,1,22]]},"reference":[{"key":"2025042808421274900_ref1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1242\/jcs.175943","article-title":"Embryo\u2013epithelium interactions during implantation at a glance","volume":"130","author":"Aplin","year":"2017","journal-title":"J. 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