{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T22:40:02Z","timestamp":1749076802438,"version":"3.41.0"},"reference-count":63,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T00:00:00Z","timestamp":1744761600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"JSPS KAKENHI","award":["JP21K06861"],"award-info":[{"award-number":["JP21K06861"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Dementia with Lewy bodies (DLB) is the second most common neurodegenerative dementia after Alzheimer's disease (AD). Early differentiation of these disorders is crucial for managing core symptoms; however, existing biomarkers remain insufficient. DLB shares motor and cognitive symptoms with Parkinson\u2019s disease (PD), and both are classified as synucleinopathies due to abnormal \u03b1-synuclein aggregation. Although \u03b1-synuclein is predominantly expressed in the central nervous system, it is also abundant in erythrocytes. Recent studies suggest a potential link between erythrocyte-derived \u03b1-synuclein and synucleinopathy pathology. Additionally, we previously reported that both erythrocytes and circulating medium and large extracellular vesicles (m\/lEVs) in plasma from healthy subjects contain full-length and C-terminally truncated \u03b1-synuclein. In this study, we found that erythrocyte \u03b1-synuclein levels were significantly lower in DLB compared to AD, PD and healthy controls. Furthermore, \u03b1-synuclein levels in circulating m\/lEVs were elevated in patients with neurodegenerative diseases. These findings provide new insights into the role of peripheral \u03b1-synuclein and suggest its potential utility as a diagnostic marker for DLB. While further validation is needed, erythrocyte-derived \u03b1-synuclein may complement nuclear medicine assessments in distinguishing DLB from other neurodegenerative disorders.<\/jats:p>","DOI":"10.1093\/jb\/mvaf017","type":"journal-article","created":{"date-parts":[[2025,4,16]],"date-time":"2025-04-16T06:53:01Z","timestamp":1744786381000},"page":"415-424","source":"Crossref","is-referenced-by-count":0,"title":["The potential of erythrocyte \u03b1-synuclein to differentiate dementia with Lewy bodies from Parkinson\u2019s and Alzheimer\u2019s diseases"],"prefix":"10.1093","volume":"177","author":[{"given":"Ryosuke","family":"Amagai","sequence":"first","affiliation":[{"name":"Laboratory of Biochemistry, Department of Biology, Faculty of Science, Toho University , Miyama 2-2-1, Funabashi, Chiba 274-8510 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryunosuke","family":"Hosoi","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry, Department of Biology, Faculty of Science, Toho University , Miyama 2-2-1, Funabashi, Chiba 274-8510 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sakura","family":"Yoshioka","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry, Department of Biology, Faculty of Science, Toho University , Miyama 2-2-1, Funabashi, Chiba 274-8510 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taiki","family":"Maruyama","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry, Department of Biology, Faculty of Science, Toho University , Miyama 2-2-1, Funabashi, Chiba 274-8510 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayuki","family":"Kawai","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Sakura Medical Center, Toho University , Shimoshizu 564-1, Sakura, Chiba 285-8741 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soroku","family":"Yagihashi","sequence":"additional","affiliation":[{"name":"Department of Exploratory Medicine on Nature, Life and Man, Faculty of Medicine, Toho University , Inagecho 5-18, Inage-ku, Chiba 263-0035,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Nukada","sequence":"additional","affiliation":[{"name":"Department of Exploratory Medicine on Nature, Life and Man, Faculty of Medicine, Toho University , Inagecho 5-18, Inage-ku, Chiba 263-0035,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryuji","family":"Sakakibara","sequence":"additional","affiliation":[{"name":"Division of Neurology, Department of Internal Medicine, Sakura Medical Center, Toho University , Shimoshizu 564-1, Sakura, Chiba 285-8741 ,","place":["Japan.  Dowakai Chiba Hospital, Hasamacho 2-508, Funabashi, Chiba 274-0822, Japan and Neurology Clinic Tsudanuma, Maeharanishi 2-14-5, Funabashi, Chiba 274-0825, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayako","family":"Okado-Matsumoto","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry, Department of Biology, Faculty of Science, Toho University , Miyama 2-2-1, Funabashi, Chiba 274-8510 ,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,4,16]]},"reference":[{"key":"2025060418163718300_ref1","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/alzrt274","article-title":"The clinical characteristics of dementia with Lewy bodies and a consideration of prodromal diagnosis","volume":"6","author":"Donaghy","year":"2014","journal-title":"Alzheimers Res. Ther."},{"key":"2025060418163718300_ref2","doi-asserted-by":"crossref","DOI":"10.1016\/j.neulet.2019.134316","article-title":"Alpha-Synuclein pathology in Parkinson's disease and related alpha-synucleinopathies","volume":"709","author":"Henderson","year":"2019","journal-title":"Neurosci. Lett."},{"key":"2025060418163718300_ref3","doi-asserted-by":"crossref","first-page":"7929","DOI":"10.1074\/jbc.C113.538249","article-title":"Evidence of native \u03b1-Synuclein conformers in the human brain","volume":"289","author":"Gould","year":"2014","journal-title":"J. Biol. Chem."},{"key":"2025060418163718300_ref4","doi-asserted-by":"crossref","first-page":"1663","DOI":"10.1126\/science.1195227","article-title":"Alpha-synuclein promotes SNARE-complex assembly in vivo and in vitro","volume":"329","author":"Burr\u00e9","year":"2010","journal-title":"Science"},{"key":"2025060418163718300_ref5","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/0896-6273(95)90302-X","article-title":"The precursor protein of non-a beta component of Alzheimer's disease amyloid is a presynaptic protein of the central nervous system","volume":"14","author":"Iwai","year":"1995","journal-title":"Neuron"},{"key":"2025060418163718300_ref6","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.bbrc.2007.04.108","article-title":"Expression of alpha-synuclein, a presynaptic protein implicated in Parkinson's disease, in erythropoietic lineage","volume":"358","author":"Nakai","year":"2007","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2025060418163718300_ref7","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1159\/000112832","article-title":"Red blood cells are the major source of alpha-synuclein in blood","volume":"5","author":"Barbour","year":"2008","journal-title":"Neurodegener. Dis."},{"key":"2025060418163718300_ref8","doi-asserted-by":"crossref","first-page":"15345","DOI":"10.1074\/jbc.M111.318949","article-title":"Alpha-Synuclein in central nervous system and from erythrocytes, mammalian cells, and Escherichia coli exists predominantly as disordered monomer","volume":"287","author":"Fauvet","year":"2012","journal-title":"J. Biol. Chem."},{"key":"2025060418163718300_ref9","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/s40478-017-0470-4","article-title":"Transmission of alpha-synuclein-containing erythrocyte-derived extracellular vesicles across the blood-brain barrier via adsorptive mediated transcytosis: another mechanism for initiation and progression of Parkinson's disease?","volume":"5","author":"Matsumoto","year":"2017","journal-title":"Acta Neuropathol. Commun."},{"key":"2025060418163718300_ref10","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.peptides.2014.09.018","article-title":"Alpha synuclein is transported into and out of the brain by the blood-brain barrier","volume":"62","author":"Sui","year":"2014","journal-title":"Peptides"},{"key":"2025060418163718300_ref11","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1093\/brain\/awv346","article-title":"Induction of \u03b1-synuclein aggregate formation by CSF exosomes from patients with Parkinson's disease and dementia with Lewy bodies","volume":"139","author":"Stuendl","year":"2016","journal-title":"Brain"},{"key":"2025060418163718300_ref12","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1002\/acn3.175","article-title":"Identification of distinct circulating exosomes in Parkinson's disease","volume":"2","author":"Tomlinson","year":"2015","journal-title":"Ann. Clin. Transl. Neurol."},{"key":"2025060418163718300_ref13","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1186\/s40478-020-00983-w","article-title":"Erythrocytic \u03b1-synuclein contained in microvesicles regulates astrocytic glutamate homeostasis: a new perspective on Parkinson's disease pathogenesis","volume":"8","author":"Sheng","year":"2020","journal-title":"Acta Neuropathol. Commun."},{"key":"2025060418163718300_ref14","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1186\/s12974-022-02413-1","article-title":"Alpha-Synuclein-containing erythrocytic extracellular vesicles: essential contributors to hyperactivation of monocytes in Parkinson's disease","volume":"19","author":"Liu","year":"2022","journal-title":"J. Neuroinflammation"},{"key":"2025060418163718300_ref15","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1093\/jb\/mvac100","article-title":"Post-translational modification of lysine residues in erythrocyte alpha-synuclein","volume":"173","author":"Amagai","year":"2023","journal-title":"J. Biochem."},{"key":"2025060418163718300_ref16","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S0197-4580(02)00065-9","article-title":"Staging of brain pathology related to sporadic Parkinson's disease","volume":"24","author":"Braak","year":"2003","journal-title":"Neurobiol. Aging"},{"key":"2025060418163718300_ref17","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1136\/jnnp.23.4.283","article-title":"The distribution of Lewy bodies in the central and autonomic nervous systems in idiopathic paralysis agitans","volume":"23","author":"Den Hartog Jager","year":"1960","journal-title":"J. Neurol. Neurosurg. Psychiatry"},{"key":"2025060418163718300_ref18","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1097\/nen.0b013e3180517454","article-title":"Analysis of the adrenal gland is useful for evaluating pathology of the peripheral autonomic nervous system in lewy body disease","volume":"66","author":"Fumimura","year":"2007","journal-title":"J. Neuropathol. Exp. Neurol."},{"key":"2025060418163718300_ref19","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1007\/BF00687767","article-title":"Parkinson's disease: the presence of Lewy bodies in Auerbach's and Meissner's plexuses","volume":"76","author":"Wakabayashi","year":"1988","journal-title":"Acta Neuropathol."},{"key":"2025060418163718300_ref20","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1016\/j.bbrc.2006.08.024","article-title":"Decreased alpha-synuclein in cerebrospinal fluid of aged individuals and subjects with Parkinson's disease","volume":"349","author":"Tokuda","year":"2006","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2025060418163718300_ref21","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1097\/WAD.0b013e31823899cc","article-title":"Alpha-synuclein in the cerebrospinal fluid differentiates synucleinopathies (Parkinson disease, dementia with Lewy bodies, multiple system atrophy) from Alzheimer disease","volume":"26","author":"Tateno","year":"2012","journal-title":"Alzheimer Dis. Assoc. Disord."},{"key":"2025060418163718300_ref22","doi-asserted-by":"crossref","first-page":"1766","DOI":"10.1212\/WNL.0b013e3181fd613b","article-title":"Detection of elevated levels of \u03b1-synuclein oligomers in CSF from patients with Parkinson disease","volume":"75","author":"Tokuda","year":"2010","journal-title":"Neurology"},{"key":"2025060418163718300_ref23","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/S1474-4422(11)70014-X","article-title":"Alpha-Synuclein and tau concentrations in cerebrospinal fluid of patients presenting with parkinsonism: a cohort study","volume":"10","author":"Mollenhauer","year":"2011","journal-title":"Lancet Neurol."},{"key":"2025060418163718300_ref24","doi-asserted-by":"crossref","first-page":"645","DOI":"10.3109\/00207454.2014.961454","article-title":"Cerebrospinal fluid alpha-synuclein as a biomarker for Parkinson's disease diagnosis: a systematic review and meta-analysis","volume":"125","author":"Gao","year":"2015","journal-title":"Int. J. Neurosci."},{"key":"2025060418163718300_ref25","doi-asserted-by":"crossref","first-page":"6001","DOI":"10.1038\/s41598-019-42399-0","article-title":"Ultrasonication-based rapid amplification of alpha-synuclein aggregates in cerebrospinal fluid","volume":"9","author":"Kakuda","year":"2019","journal-title":"Sci. Rep."},{"key":"2025060418163718300_ref26","doi-asserted-by":"crossref","first-page":"7669","DOI":"10.1007\/s00216-016-9863-7","article-title":"Total alpha-synuclein levels in human blood cells, CSF, and saliva determined by a lipid-ELISA","volume":"408","author":"Abd-Elhadi","year":"2016","journal-title":"Anal. Bioanal. Chem."},{"key":"2025060418163718300_ref27","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1136\/jnnp-2016-314857","article-title":"Plasma alpha-synuclein predicts cognitive decline in Parkinson's disease","volume":"88","author":"Lin","year":"2017","journal-title":"J. Neurol. Neurosurg. Psychiatry"},{"key":"2025060418163718300_ref28","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.neulet.2010.06.009","article-title":"Significance and confounders of peripheral DJ-1 and alpha-synuclein in Parkinson's disease","volume":"480","author":"Shi","year":"2010","journal-title":"Neurosci. Lett."},{"key":"2025060418163718300_ref29","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1159\/000506480","article-title":"A comparative study of the diagnostic potential of plasma and Erythrocytic alpha-Synuclein in Parkinson's disease","volume":"19","author":"Wang","year":"2020","journal-title":"Neurodegener. Dis."},{"key":"2025060418163718300_ref30","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.neulet.2015.05.030","article-title":"Detection of alpha-synuclein oligomers in red blood cells as a potential biomarker of Parkinson's disease","volume":"599","author":"Wang","year":"2015","journal-title":"Neurosci. Lett."},{"key":"2025060418163718300_ref31","doi-asserted-by":"crossref","first-page":"1437622","DOI":"10.3389\/fnagi.2024.1437622","article-title":"Correlations of erythrocytic oligomer \u03b1-synuclein levels with age, sex and clinical variables in patients with Parkinson's disease","volume":"16","author":"Lu","year":"2024","journal-title":"Front. Aging Neurosci."},{"key":"2025060418163718300_ref32","doi-asserted-by":"crossref","first-page":"11120","DOI":"10.1038\/srep11120","article-title":"Total and proteinase K-resistant alpha-Synuclein levels in erythrocytes, determined by their ability to bind phospholipids, associate with Parkinson's disease","volume":"5","author":"Abd-Elhadi","year":"2015","journal-title":"Sci. Rep."},{"key":"2025060418163718300_ref33","doi-asserted-by":"crossref","first-page":"13713","DOI":"10.1038\/s41598-017-14175-5","article-title":"Posttranslational modifications of blood-derived alpha-synuclein as biochemical markers for Parkinson's disease","volume":"7","author":"Vicente Miranda","year":"2017","journal-title":"Sci. Rep."},{"key":"2025060418163718300_ref34","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1093\/jb\/mvae012","article-title":"C-terminally truncation is a prominent post-translational modification of human erythrocyte \u03b1-synuclein","volume":"175","author":"Amagai","year":"2024","journal-title":"J. Biochem."},{"key":"2025060418163718300_ref35","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1212\/WNL.34.7.939","article-title":"Clinical diagnosis of Alzheimer's disease: report of the NINCDS-ADRDA work group under the auspices of Department of Health and Human Services Task Force on Alzheimer's disease","volume":"34","author":"McKhann","year":"1984","journal-title":"Neurology"},{"key":"2025060418163718300_ref36","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.1002\/mds.26424","article-title":"MDS clinical diagnostic criteria for Parkinson's disease","volume":"30","author":"Postuma","year":"2015","journal-title":"Mov. Disord."},{"key":"2025060418163718300_ref37","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1212\/WNL.0000000000004058","article-title":"Diagnosis and management of dementia with Lewy bodies: fourth consensus report of the DLB consortium","volume":"89","author":"McKeith","year":"2017","journal-title":"Neurology"},{"key":"2025060418163718300_ref38","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.parkreldis.2011.02.001","article-title":"Sensitivity and specificity of metaiodobenzylguanidine (MIBG) myocardial accumulation in the diagnosis of Lewy body diseases in a movement disorder clinic","volume":"17","author":"Tateno","year":"2011","journal-title":"Parkinsonism Relat. Disord."},{"key":"2025060418163718300_ref39","doi-asserted-by":"crossref","first-page":"400","DOI":"10.3906\/sag-2008-253","article-title":"Dopamine transporter SPECT imaging in Parkinson?s disease and parkinsonian disorders","volume":"51","author":"Akdemir","year":"2021","journal-title":"Turk. J. Med. Sci."},{"key":"2025060418163718300_ref40","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1002\/gps.4229","article-title":"Diagnostic value of MIBG cardiac scintigraphy for differential dementia diagnosis","volume":"30","author":"Slaets","year":"2015","journal-title":"Int. J. Geriatr. Psychiatry"},{"key":"2025060418163718300_ref41","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0120540","article-title":"Diagnostic accuracy of I-123-meta-iodobenzylguanidine myocardial scintigraphy in dementia with Lewy bodies: a multicenter study","volume":"10","author":"Yoshita","year":"2015","journal-title":"PLoS One"},{"key":"2025060418163718300_ref42","first-page":"9","article-title":"Measurement of the dopaminergic degeneration in Parkinson's disease with [123I] beta-CIT and SPECT. Correlation with clinical findings and comparison with multiple system atrophy and progressive supranuclear palsy","volume":"50","author":"Br\u00fccke","year":"1997","journal-title":"J. Neural Transm."},{"key":"2025060418163718300_ref43","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1007\/s002590100571","article-title":"Striatal dopamine transporter function in dementia with Lewy bodies and Parkinson's disease","volume":"28","author":"Ransmayr","year":"2001","journal-title":"Eur. J. Nucl. Med."},{"key":"2025060418163718300_ref44","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1001\/archneur.61.6.919","article-title":"Dopamine transporter loss visualized with FP-CIT SPECT in the differential diagnosis of dementia with Lewy bodies","volume":"61","author":"O'Brien","year":"2004","journal-title":"Arch. Neurol."},{"key":"2025060418163718300_ref45","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1007\/s00259-006-0155-x","article-title":"Quantification of [123I]FP-CIT SPECT brain images: an accurate technique for measurement of the specific binding ratio","volume":"33","author":"Tossici-Bolt","year":"2006","journal-title":"Eur. J. Nucl. Med. Mol. Imaging"},{"key":"2025060418163718300_ref46","doi-asserted-by":"crossref","first-page":"4243","DOI":"10.1007\/s00784-019-02867-3","article-title":"Characterization of circulating DNA in plasma of patients after allogeneic bone grafting","volume":"23","author":"Solakoglu","year":"2019","journal-title":"Clin. Oral Investig."},{"key":"2025060418163718300_ref47","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.jim.2014.06.007","article-title":"Isolation of biologically-active exosomes from human plasma","volume":"411","author":"Muller","year":"2014","journal-title":"J. Immunol. Methods"},{"key":"2025060418163718300_ref48","doi-asserted-by":"crossref","first-page":"20360","DOI":"10.3402\/jev.v2i0.20360","article-title":"Standardization of sample collection, isolation and analysis methods in extracellular vesicle research","volume":"2","author":"Witwer","year":"2013","journal-title":"J. Extracell. Vesicles"},{"key":"2025060418163718300_ref49","doi-asserted-by":"crossref","DOI":"10.1080\/20013078.2018.1535750","article-title":"Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines","volume":"7","author":"Th\u00e9ry","year":"2018","journal-title":"J. Extracell. Vesicles"},{"key":"2025060418163718300_ref50","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1074\/mcp.M700076-MCP200","article-title":"High resolution clear native electrophoresis for in-gel functional assays and fluorescence studies of membrane protein complexes","volume":"6","author":"Wittig","year":"2007","journal-title":"Mol. Cell. Proteomics"},{"key":"2025060418163718300_ref51","first-page":"6","article-title":"Mitochondrial respiratory chain supercomplexes in human mononuclear leukocytes","volume":"4","author":"Hara","year":"2016","journal-title":"Int. J. Anal. Bio-Sci."},{"key":"2025060418163718300_ref52","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1093\/jb\/mvad100","article-title":"Supercomplex formation of mitochondrial respiratory chain complexes in leukocytes from patients with neurodegenerative diseases","volume":"175","author":"Hara","year":"2024","journal-title":"J. Biochem."},{"key":"2025060418163718300_ref53","article-title":"Use of in-gel peroxidase assay for cytochrome c to visualize mitochondrial complexes III and IV","volume":"9","author":"Hara","year":"2020","journal-title":"Biol. Open"},{"key":"2025060418163718300_ref54","doi-asserted-by":"crossref","first-page":"14211","DOI":"10.1038\/srep14211","article-title":"Sensitive western blotting for detection of endogenous Ser129-phosphorylated alpha-synuclein in intracellular and extracellular spaces","volume":"5","author":"Sasaki","year":"2015","journal-title":"Sci. Rep."},{"key":"2025060418163718300_ref55","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1038\/bmt.2012.244","article-title":"Investigation of the freely available easy-to-use software 'EZR' for medical statistics","volume":"48","author":"Kanda","year":"2013","journal-title":"Bone Marrow Transplant."},{"key":"2025060418163718300_ref56","doi-asserted-by":"crossref","DOI":"10.1136\/bmjopen-2014-005122","article-title":"Is ioflupane I123 injection diagnostically effective in patients with movement disorders and dementia? Pooled analysis of four clinical trials","volume":"4","author":"O'Brien","year":"2014","journal-title":"BMJ Open"},{"key":"2025060418163718300_ref57","doi-asserted-by":"crossref","first-page":"53","DOI":"10.3389\/fnmol.2018.00053","article-title":"\u0391-Synuclein Heterocomplexes with \u03b2-amyloid are increased in red blood cells of Parkinson's disease patients and correlate with disease severity","volume":"11","author":"Daniele","year":"2018","journal-title":"Front. Mol. 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