{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T11:41:08Z","timestamp":1779882068208,"version":"3.53.1"},"reference-count":59,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T00:00:00Z","timestamp":1751414400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"program of Inter-University Research Network for High-Depth Omics, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University"},{"name":"Japanese Science and Technology Agency (JST) PRESTO","award":["JPMJPR24N4"],"award-info":[{"award-number":["JPMJPR24N4"]}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for Promotion of Science","doi-asserted-by":"crossref","award":["24H02030, 18H05552"],"award-info":[{"award-number":["24H02030, 18H05552"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for Promotion of Science","doi-asserted-by":"crossref","award":["21H04774 and 24H00567"],"award-info":[{"award-number":["21H04774 and 24H00567"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for Promotion of Science","doi-asserted-by":"crossref","award":["26116730, 18K06240 and 23K05777"],"award-info":[{"award-number":["26116730, 18K06240 and 23K05777"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>S-adenosylmethionine (SAM) is the major cellular methyl donor and regulates gene expression through epigenetic and other methylation-related processes. While SAM biosynthesis influences a variety of biological phenomena including ageing and disease, its cell type-specific regulation and functional implications remain poorly understood. In this study, we report that the Drosophila germline exhibits a uniquely repressive SAM biosynthesis status during gametogenesis, as indicated by low expression of SAM synthetase (Sam-S), a key enzyme for SAM production. Experimentally enhancing SAM biosynthesis in the germline led to increased expression of retrotransposons, with HeT-A, a telomere-specific element, showing the most pronounced response. We also observed increased promoter activity of HeT-A under high SAM conditions, along with accumulation of N6-methyladenine (6\u00a0mA), the major form of DNA methylation in the Drosophila genome. Although a direct causal link between 6\u00a0mA levels and transcription was not broadly observed across other retrotransposons or genes, these results raise the possibility that SAM levels modulate HeT-A expression at least in part through DNA methylation. Our findings highlight a previously underexplored metabolic feature of the Drosophila germline and suggest that SAM availability contributes to the regulation of retrotransposon activity in a lineage-specific manner.<\/jats:p>","DOI":"10.1093\/jb\/mvaf041","type":"journal-article","created":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T04:39:40Z","timestamp":1751431180000},"page":"217-228","source":"Crossref","is-referenced-by-count":2,"title":["Repressive\n                    <i>S<\/i>\n                    -adenosylmethionine biosynthesis status inhibits transcription of\n                    <i>HeT-A<\/i>\n                    retrotransposon in the germline of\n                    <i>Drosophila<\/i>"],"prefix":"10.1093","volume":"178","author":[{"given":"Yoshiki","family":"Hayashi","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Science, Kyushu University , 744, Moto-oka, Nishi-ku, Fukuoka 819-0395 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinjiro","family":"Hino","sequence":"additional","affiliation":[{"name":"Department of Medical Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University , 2-2-1, Honjo, Chuo-ku, Kumamoto 860-0811 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuya","family":"Sato","sequence":"additional","affiliation":[{"name":"Central Research Facility, Faculty of Medicine, Saitama Medical University , 1397-1, Yamane, Hidaka, Saitama 350-1241 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soshiro","family":"Kashio","sequence":"additional","affiliation":[{"name":"Department of Integrative Bioanalytics, Institute of Development Aging and Cancer (IDAC), Tohoku University , 4-1, Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575 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