{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:24:32Z","timestamp":1787009072007,"version":"build-2736575974"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T00:00:00Z","timestamp":1760313600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Suzuken Memorial Foundation; the Mochida Memorial Foundation; and the Takeda Science Foundation"},{"name":"the Keio University Fukuzawa Fund; Medical Research Center Initiative for High Depth Omics, the Chemo-Sero-Therapeutic Research Institute"},{"name":"the Japanese Society of Hematology; the Keio University Academic Development Fund"},{"name":"the Kobayashi Foundation; the Friends of Leukemia Research Fund"},{"name":"JSPS KAKENHI","award":["23\u00a0K07851"],"award-info":[{"award-number":["23\u00a0K07851"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The murine retrovirus integration site 1 (MRVI1) gene encodes an endoplasmic reticulum (ER)-associated membrane protein involved in calcium signalling, yet its molecular interaction network remains largely undefined. Here, we employed TurboID-based proximity labelling to construct the first comprehensive map of MRVI1-associated proteins in mammalian cells. This analysis identified &amp;gt;700 candidate interactors, including ER-localized factors and components of intracellular trafficking, consistent with the subcellular localization and signalling role of MRVI1. To investigate oncogenic modulation, we examined how co-expression of NPM\u2013ALK\u2014a constitutively active tyrosine kinase implicated in lymphoid malignancies\u2014reshapes the MRVI1 interactome. Quantitative proteomics revealed that while the overall composition of MRVI1-associated proteins was largely preserved, a subset of interactions was selectively enhanced or attenuated by NPM\u2013ALK. The association of MRVI1 with several signalling-related proteins was enhanced by NPM\u2013ALK, including 12 proteins that have all been previously implicated in cancer-related pathways. In contrast, proteins whose interaction with MRVI1 was suppressed were functionally enriched in the Gene Ontology term \u2018negative regulation of apoptotic process\u2019. Notably, anti-apoptotic regulators such as DDB1, PHB2 and NOTCH2 showed significantly reduced proximity labelling, suggesting that MRVI1 may participate in apoptosis-related networks disrupted during oncogenic transformation. Together, our findings demonstrate that MRVI1 forms a functionally diverse protein network that can be selectively remodelled by oncogenic signalling. This study not only uncovers potential mechanisms by which MRVI1 contributes to transformation but also provides a valuable proteomic resource for future investigation of MRVI1 function and regulation.<\/jats:p>","DOI":"10.1093\/jb\/mvaf057","type":"journal-article","created":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T12:04:49Z","timestamp":1760097889000},"page":"415-434","source":"Crossref","is-referenced-by-count":3,"title":["Identification of MRVI1-interacting proteins by biotin-based proximity labelling reveals NPM\u2013ALK-dependent interaction dynamics"],"prefix":"10.1093","volume":"178","author":[{"given":"Kosuke","family":"Higashi","sequence":"first","affiliation":[{"name":"Division of Hygienic Chemistry, Faculty of Pharmacy, Keio University , 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuuki","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Division of Hygienic Chemistry, Faculty of Pharmacy, Keio University , 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidetaka","family":"Kosako","sequence":"additional","affiliation":[{"name":"Division of Cell Signaling, Institute of Advanced Medical Sciences, Tokushima University , 3-18-15 Kuramoto-cho, Tokushima 770-8503 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2136-4562","authenticated-orcid":false,"given":"Kazumasa","family":"Aoyama","sequence":"additional","affiliation":[{"name":"Division of Hygienic Chemistry, Faculty of Pharmacy, Keio University , 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,10,24]]},"reference":[{"key":"2025120404515370200_ref1","doi-asserted-by":"crossref","first-page":"2069","DOI":"10.1038\/sj.onc.1202419","article-title":"Mrvi1, a common MRV integration site in BXH2 myeloid leukemias, encodes a protein with homology to a lymphoid-restricted membrane protein Jaw1","volume":"18","author":"Shaughnessy","year":"1999","journal-title":"Oncogene"},{"key":"2025120404515370200_ref2","doi-asserted-by":"crossref","first-page":"24153","DOI":"10.1074\/jbc.M101530200","article-title":"Molecular determinants of the interaction between the inositol 1,4,5-trisphosphate receptor-associated cGMP kinase substrate (IRAG) and cGMP kinase Ibeta","volume":"276","author":"Ammendola","year":"2001","journal-title":"J. Biol. Chem."},{"key":"2025120404515370200_ref3","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1038\/35004606","article-title":"Regulation of intracellular calcium by a signalling complex of IRAG, IP3 receptor and cGMP kinase Ibeta","volume":"404","author":"Schlossmann","year":"2000","journal-title":"Nature"},{"key":"2025120404515370200_ref4","doi-asserted-by":"crossref","DOI":"10.3390\/ijms24129837","article-title":"Novel functional features of cGMP substrate proteins IRAG1 and IRAG2","volume":"24","author":"Pr\u00fcschenk","year":"2023","journal-title":"Int. J. Mol. 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