{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T17:48:36Z","timestamp":1792518516166,"version":"4.5.0"},"reference-count":74,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:00:00Z","timestamp":1790294400000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"JST SPRING","award":["JPMJSP2136"],"award-info":[{"award-number":["JPMJSP2136"]}]},{"name":"JSPS KAKENHI","award":["22\u00a0K15036"],"award-info":[{"award-number":["22\u00a0K15036"]}]},{"name":"JSPS KAKENHI","award":["24\u00a0K09328"],"award-info":[{"award-number":["24\u00a0K09328"]}]},{"name":"JSPS KAKENHI","award":["20H03186"],"award-info":[{"award-number":["20H03186"]}]},{"name":"JSPS KAKENHI","award":["20H05392"],"award-info":[{"award-number":["20H05392"]}]},{"name":"JSPS KAKENHI","award":["22H04697"],"award-info":[{"award-number":["22H04697"]}]},{"name":"JSPS KAKENHI","award":["24\u00a0K01956"],"award-info":[{"award-number":["24\u00a0K01956"]}]},{"name":"MEXT KAKENHI","award":["24H02328"],"award-info":[{"award-number":["24H02328"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In eukaryotes, single-stranded DNA (ssDNA) generated during DNA replication, recombination and repair is rapidly bound and protected by the major ssDNA-binding protein replication protein A (RPA). RPA not only stabilizes ssDNA but also acts as a central platform that coordinates diverse DNA transactions. Exhaustion of RPA due to unregulated ssDNA production leads to replication fork breakage and replication catastrophe, underscoring its critical role in genome stability. However, the direct consequences of RPA limitation remain incompletely understood. Using Xenopus egg extracts, we show that excess ssDNA induces spontaneous priming, a reaction that is otherwise prevented in a physiological nuclear environment. We provide evidence that priming suppression is mediated by stoichiometric binding of RPA to ssDNA. Analysis of the ssDNA-binding proteome reveals that RPA promotes the association of ataxia telangiectasia and Rad3-related protein (ATR) checkpoint factors, Pol\u03b1\u2013primase and the RFWD3 ubiquitin ligase with ssDNA. In contrast, RPA depletion induces the recruitment of Rad51, Rad51 paralogs and Fbh1, a DNA helicase that interacts with both RPA and Rad51 and promotes fork breakage under replication stress. Collectively, our findings suggest that RPA contributes to genome stability by protecting ssDNA from inappropriate DNA synthesis and unscheduled recruitment of recombination and fork-processing factors.<\/jats:p>","DOI":"10.1093\/jb\/mvag066","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T11:29:24Z","timestamp":1790335764000},"source":"Crossref","is-referenced-by-count":0,"title":["Replication protein A prevents unregulated priming and Rad51 loading on single-stranded DNA in nuclear extracts of\n                    <i>Xenopus<\/i>\n                    eggs"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9788-7765","authenticated-orcid":false,"given":"Taisei","family":"Miyata","sequence":"first","affiliation":[{"name":"Graduate School of Systems Life Sciences, Kyushu University , 744 Motooka, Nishi-ku , Fukuoka, 819-0395,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Tani","sequence":"additional","affiliation":[{"name":"Liaison Laboratory Research Promotion Center, Institute of Molecular Embryology and Genetics, Kumamoto University , 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0925-2004","authenticated-orcid":false,"given":"Yoshitaka","family":"Kawasoe","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, Kyushu University , 744 Motooka, Nishi-ku , Fukuoka, 819-0395,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7515-1511","authenticated-orcid":false,"given":"Kei-ichiro","family":"Ishiguro","sequence":"additional","affiliation":[{"name":"Department of Chromosome Biology, Institute of Molecular Embryology and Genetics, Kumamoto University , 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811 ,","place":["Japan"]},{"name":"Department of Comparative Pathology and Biomedical Science, Graduate School of Medicine, Chiba University , 1-8-1 Inohana, Chuo-ku, Chiba 260-8670 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1947-7680","authenticated-orcid":false,"given":"Tatsuro S","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science, Kyushu University , 744 Motooka, Nishi-ku , Fukuoka, 819-0395,","place":["Japan"]},{"name":"Quantum and Spacetime Research Institute, Kyushu University , 744 Motooka, Nishi-ku, Fukuoka 819-0395 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"key":"2026102013363229500_ref1","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1146\/annurev.biochem.66.1.61","article-title":"Replication protein A: a heterotrimeric, single-stranded DNA-binding protein required for eukaryotic DNA metabolism","volume":"66","author":"Wold","year":"1997","journal-title":"Annu. 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