{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T13:11:14Z","timestamp":1781788274098,"version":"3.54.5"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2019,4,20]],"date-time":"2019-04-20T00:00:00Z","timestamp":1555718400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81872972"],"award-info":[{"award-number":["81872972"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Basic Scientific Research Funding Project of Beijing University of Chinese Medicine","award":["2015-JYB-JSM S026"],"award-info":[{"award-number":["2015-JYB-JSM S026"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Vinegar soaked black soybean is a traditional Chinese food widely used for the treatment of hypertension. While its pharmacodynamic substance was not fully unveiled. It contained abundant glutelin, thus the purpose of this study was to obtain potent antihypertensive peptides from vinegar soaked black soybean. Black soybean was soaked with vinegar and then glutelin was first catalyzed by alcalase. Ultrafiltration, ion exchange chromatography and reversed-phase high performance liquid chromatography were sequentially applied to separate and purify the angiotensin-I converting enzyme (ACE) inhibitory peptides from glutelin hydrolysates. As a result, the fraction L1-4 with the highest ACE inhibitory activity (83.41%) at the final concentration of 0.01 mg\/ml was obtained and five peptides were then identified. These peptides were further optimized by virtual screening combining with in silico proteolysis. Finally, a novel tetrapeptide Phe-Gly-Ser-Phe (FGSF) was obtained. FGSF exhibited high in vitro ACE inhibitory activity (IC50 = 117.11 \u03bcM) and in vivo hypotensive effect which maximally reduced systolic blood pressure of 21.95 mmHg at 20 mg\/kg body weight in spontaneously hypertensive rats. Our study demonstrated that FGSF derived from vinegar soaked black soybean might be used as a promising ingredient for pharmaceuticals against hypertension and its related diseases.<\/jats:p>","DOI":"10.1093\/jb\/mvz029","type":"journal-article","created":{"date-parts":[[2019,4,14]],"date-time":"2019-04-14T19:07:19Z","timestamp":1555268839000},"page":"223-230","source":"Crossref","is-referenced-by-count":21,"title":["A novel angiotensin-I converting enzyme inhibitory peptide derived from the glutelin of vinegar soaked black soybean and its antihypertensive effect in spontaneously hypertensive rats"],"prefix":"10.1093","volume":"166","author":[{"given":"Yueyuan","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Life Science, Beijing University of Chinese Medicine, No. 11, Bei San Huan Dong Lu, Chaoyang District, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanling","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 11, Bei San Huan Dong Lu, Chaoyang District, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiyao","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Life Science, Beijing University of Chinese Medicine, No. 11, Bei San Huan Dong Lu, Chaoyang District, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengjue","family":"Shu","sequence":"additional","affiliation":[{"name":"ICF International Inc., 3 Corporate Blvd, NE#100, Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Science, Beijing University of Chinese Medicine, No. 11, Bei San Huan Dong Lu, Chaoyang District, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liansheng","family":"Qiao","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica, Beijing University of Chinese Medicine, No. 11, Bei San Huan Dong Lu, Chaoyang District, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zujun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Cardiovascular Institute and Fuwai Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, No. 167 North Lishi Road, Xicheng District, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingzhi","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Life Science, Beijing University of Chinese Medicine, No. 11, Bei San Huan Dong Lu, Chaoyang District, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,4,20]]},"reference":[{"key":"2019082010251447900_mvz029-B1","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1007\/s12170-011-0181-5","article-title":"Dairy consumption, blood pressure, and risk of hypertension: an evidence-based review of recent literature","volume":"5","author":"Mcgrane","year":"2011","journal-title":"Curr. 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