{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T17:44:40Z","timestamp":1792431880666,"version":"4.5.0"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2020,3,2]],"date-time":"2020-03-02T00:00:00Z","timestamp":1583107200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Facial burns present a challenge in burn care, as hypertrophic scarring and dyspigmentation can interfere with patients\u2019 personal identities, ocular and oral functional outcomes, and have long-term deleterious effects. The purpose of this study is to evaluate our initial experience with non-cultured, autologous skin cell suspension (ASCS) for the treatment of deep partial-thickness (DPT) facial burns. Patients were enrolled at a single burn center during a multicenter, prospective, single-arm, observational study involving the compassionate use of ASCS for the treatment of large total BSA (TBSA) burns. Treatment decisions concerning facial burns were made by the senior author. Facial burns were initially excised and treated with allograft. The timing of ASCS application was influenced by an individual\u2019s clinical status; however, all patients were treated within 30 days of injury. Outcomes included subjective cosmetic parameters and the number of reoperations within 3 months. Five patients (4 males, 1 female) were treated with ASCS for DPT facial burns. Age ranged from 2.1 to 40.7 years (mean 18.2 \u00b1 17.3 years). Average follow-up was 231.2 \u00b1 173.1 days (range 63\u2013424 days). Two patients required reoperation for partial graft loss within 3 months in areas of full-thickness injury. There were no major complications and one superficial hematoma. Healing and cosmetic outcomes were equivalent to, and sometimes substantially better than, outcomes typical of split-thickness autografting. Non-cultured, ASCS was successfully used to treat DPT facial burns containing confluent dermis with remarkable cosmetic outcomes. Treatment of DPT burns with ASCS may be an alternative to current treatments, particularly in patients prone to dyspigmentation, scarring sequelae, and with limited donor sites.<\/jats:p>","DOI":"10.1093\/jbcr\/iraa037","type":"journal-article","created":{"date-parts":[[2020,3,2]],"date-time":"2020-03-02T08:19:58Z","timestamp":1583137198000},"page":"1045-1051","source":"Crossref","is-referenced-by-count":8,"title":["Initial Experience With Autologous Skin Cell Suspension for Treatment of Deep Partial-Thickness Facial Burns"],"prefix":"10.1093","volume":"41","author":[{"given":"Joseph A","family":"Molnar","sequence":"first","affiliation":[{"name":"Department of Plastic & Reconstructive Surgery, Winston-Salem, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Walker","sequence":"additional","affiliation":[{"name":"Department of Plastic & Reconstructive Surgery, Winston-Salem, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas N","family":"Steele","sequence":"additional","affiliation":[{"name":"Department of Plastic & Reconstructive Surgery, Winston-Salem, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Craig","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Wake Forest University School of Medicine, Winston-Salem, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Wake Forest University School of Medicine, Winston-Salem, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey E","family":"Carter","sequence":"additional","affiliation":[{"name":"Department of Surgery, Louisiana State University Health Science Center, New Orleans, Louisiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"IV","given":"James H","family":"Holmes","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Wake Forest University School of Medicine, Winston-Salem, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,3,2]]},"reference":[{"key":"2020092312344093100_CIT0001","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1016\/j.burns.2007.10.008","article-title":"Coping style, personality and adolescent adjustment 10 years post-burn","volume":"34","author":"Liber","year":"2008","journal-title":"Burns"},{"key":"2020092312344093100_CIT0002","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1016\/j.burns.2006.02.025","article-title":"The use of cultured epithelial autograft in the treatment of major burn wounds: eleven years of clinical experience","volume":"32","author":"Wood","year":"2006","journal-title":"Burns"},{"key":"2020092312344093100_CIT0003","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1097\/00004630-200021060-00007","article-title":"Sprayed keratinocyte suspensions accelerate epidermal coverage in a porcine microwound model","volume":"21","author":"Navarro","year":"2000","journal-title":"J Burn Care Rehabil"},{"key":"2020092312344093100_CIT0004","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.burns.2011.03.001","article-title":"Characterisation of the cell suspension harvested from the dermal epidermal junction using a ReCell\u00ae kit","volume":"38","author":"Wood","year":"2012","journal-title":"Burns"},{"key":"2020092312344093100_CIT0005","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1093\/jbcr\/iry029","article-title":"A comparative study of the ReCell\u00ae device and autologous spit-thickness meshed skin graft in the treatment of acute burn injuries","volume":"39","author":"Holmes Iv","year":"2018","journal-title":"J Burn Care Res"},{"key":"2020092312344093100_CIT0006","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1016\/j.burns.2007.04.011","article-title":"A randomized trial comparing ReCell system of epidermal cells delivery versus classic skin grafts for the treatment of deep partial thickness burns","volume":"33","author":"Gravante","year":"2007","journal-title":"Burns"},{"key":"2020092312344093100_CIT0007","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1016\/j.burns.2018.11.002","article-title":"Demonstration of the safety and effectiveness of the RECELL\u00ae system combined with split-thickness meshed autografts for the reduction of donor skin to treat mixed-depth burn injuries","volume":"45","author":"Holmes","year":"2019","journal-title":"Burns"},{"key":"2020092312344093100_CIT0008","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1016\/j.burns.2011.12.020","article-title":"A prospective randomised clinical pilot study to compare the effectiveness of Biobrane\u00ae synthetic wound dressing, with or without autologous cell suspension, to the local standard treatment regimen in paediatric scald injuries","volume":"38","author":"Wood","year":"2012","journal-title":"Burns"},{"key":"2020092312344093100_CIT0009","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1016\/j.burns.2017.01.015","article-title":"Evolution of skin grafting for treatment of burns: Reverdin pinch grafting to Tanner mesh grafting and beyond","volume":"43","author":"Singh","year":"2017","journal-title":"Burns"},{"key":"2020092312344093100_CIT0010","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1097\/00004630-200101000-00009","article-title":"Melanocyte repopulation in full-thickness wounds using a cell spray apparatus","volume":"22","author":"Navarro","year":"2001","journal-title":"J Burn Care Rehabil"},{"key":"2020092312344093100_CIT0011","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/j.burns.2006.10.388","article-title":"The use of a non-cultured autologous cell suspension and Integra dermal regeneration template to repair full-thickness skin wounds in a porcine model: a one-step process","volume":"33","author":"Wood","year":"2007","journal-title":"Burns"},{"issue":"Pt 1","key":"2020092312344093100_CIT0012","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1097\/00004630-200021010-00010","article-title":"The treatment of hypopigmented lesions with cultured epithelial autograft","volume":"21","author":"Stoner","year":"2000","journal-title":"J Burn Care Rehabil"},{"key":"2020092312344093100_CIT0013","first-page":"31","article-title":"A comparative study of spray keratinocytes and autologous meshed split-thickness skin graft in the treatment of acute burn injuries","volume":"27","author":"Sood","year":"2015","journal-title":"Wounds"},{"key":"2020092312344093100_CIT0014","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1001\/jamadermatol.2017.5795","article-title":"Combination of follicular and epidermal cell suspension as a novel surgical approach in difficult-to-treat vitiligo: a randomized clinical trial","volume":"154","author":"Razmi","year":"2018","journal-title":"JAMA Dermatol"},{"key":"2020092312344093100_CIT0015","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.jdermsci.2017.10.007","article-title":"A single-arm open-label clinical trial of autologous epidermal cell transplantation for stable vitiligo: a 30-month follow-up","volume":"89","author":"Orouji","year":"2018","journal-title":"J Dermatol Sci"},{"key":"2020092312344093100_CIT0016","first-page":"312","article-title":"Evaluation of treatment response to autologous transplantation of noncultured melanocyte \/ keratinocyte cell suspension in patients with stable vitiligo","volume-title":"An Bras Dermatol","author":"Ramos","year":"2017"},{"key":"2020092312344093100_CIT0017","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.jtv.2015.11.003","article-title":"Autologous epidermal cell suspension: a promising treatment for chronic wounds","volume":"25","author":"Zhao","year":"2016","journal-title":"J Tissue Viability"},{"key":"2020092312344093100_CIT0018","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/S0305-4179(96)00025-3","article-title":"Management of hypopigmentation following burn injury","volume":"22","author":"Grover","year":"1996","journal-title":"Burns"},{"key":"2020092312344093100_CIT0019","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1016\/S0305-4179(03)00078-0","article-title":"Laser Doppler imaging determines need for excision and grafting in advance of clinical judgment: a prospective blinded trial","volume":"29","author":"Jeng","year":"2003","journal-title":"Burns"},{"key":"2020092312344093100_CIT0020","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.burns.2008.08.011","article-title":"Accuracy of early burn depth assessment by laser Doppler imaging on different days post burn","volume":"35","author":"Hoeksema","year":"2009","journal-title":"Burns"},{"key":"2020092312344093100_CIT0021","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/S0305-4179(03)00067-6","article-title":"What is the prevalence of hypertrophic scarring following burns?","volume":"29","author":"Bombaro","year":"2003","journal-title":"Burns"},{"key":"2020092312344093100_CIT0022","doi-asserted-by":"crossref","first-page":"3523","DOI":"10.1002\/term.2264","article-title":"Restoring full-thickness defects with spray skin in conjunction with dermal regenerate template and split-thickness skin grafting: a pilot study","volume":"11","author":"Hammer","year":"2017","journal-title":"J Tissue Eng Regen Med"},{"key":"2020092312344093100_CIT0023","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.1016\/j.burns.2015.05.004","article-title":"Uncultured adipose-derived regenerative cells (ADRCs) seeded in collagen scaffold improves dermal regeneration, enhancing early vascularization and structural organization following thermal burns","volume":"41","author":"Foubert","year":"2015","journal-title":"Burns"},{"key":"2020092312344093100_CIT0024","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1016\/j.burns.2016.11.007","article-title":"Modified Vancouver Scar Scale score is linked with quality of life after burn","volume":"43","author":"Finlay","year":"2017","journal-title":"Burns"},{"key":"2020092312344093100_CIT0025","doi-asserted-by":"crossref","first-page":"1960","DOI":"10.1097\/01.PRS.0000122207.28773.56","article-title":"The patient and observer scar assessment scale: a reliable and feasible tool for scar evaluation","volume":"113","author":"Draaijers","year":"2004","journal-title":"Plast Reconstr Surg"},{"key":"2020092312344093100_CIT0026","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1364\/BOE.9.001809","article-title":"Burn-injured tissue detection for debridement surgery through the combination of non-invasive optical imaging techniques","volume":"9","author":"Heredia-Juesas","year":"2018","journal-title":"Biomed Opt Express"}],"container-title":["Journal of Burn Care &amp; Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jbcr\/advance-article-pdf\/doi\/10.1093\/jbcr\/iraa037\/33219675\/iraa037.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jbcr\/article-pdf\/41\/5\/1045\/33784552\/iraa037.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jbcr\/article-pdf\/41\/5\/1045\/33784552\/iraa037.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T12:35:39Z","timestamp":1600864539000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jbcr\/article\/41\/5\/1045\/5771293"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,2]]},"references-count":26,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2020,3,2]]},"published-print":{"date-parts":[[2020,9,23]]}},"URL":"https:\/\/doi.org\/10.1093\/jbcr\/iraa037","relation":{},"ISSN":["1559-047X","1559-0488"],"issn-type":[{"value":"1559-047X","type":"print"},{"value":"1559-0488","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,9]]},"published":{"date-parts":[[2020,3,2]]}}}