{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T20:25:42Z","timestamp":1780604742445,"version":"3.54.1"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2020,8,25]],"date-time":"2020-08-25T00:00:00Z","timestamp":1598313600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100003995","name":"Natural Science Foundation of Anhui Province","doi-asserted-by":"publisher","award":["1508085SMH231"],"award-info":[{"award-number":["1508085SMH231"]}],"id":[{"id":"10.13039\/501100003995","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Research and Development Plan","award":["1804h08020230"],"award-info":[{"award-number":["1804h08020230"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,3,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The mechanism underlying burn injury-induced enhanced vascular endothelial permeability and consequent body fluid extravasation is unclear. Here, the rat aortic endothelial cells (RAECs) were treated with the serum derived from rats with burn injury to elucidate the mechanism. Sprague\u2013Dawley (SD) rats were grouped as follows (10 rats\/group): control, 2, 4, 8, 12, and 24 hours postburn groups. The heart, liver, kidney, lung, jejunum, and ileum of rats injected with 2% Evans blue (EB) through the tail vein were excised to detect the EB level in each organ. The serum levels of hypoxia-inducible factor-1\u03b1 (HIF-1\u03b1) and endothelin-1 (ET-1) were examined using enzyme-linked immunosorbent assay (ELISA). The effect of serum from 12-hour postburn group on the membrane permeability of RAEC monolayer, as well as on the mRNA and protein levels of ET-1, endothelin receptor A (ETA), ETB, and zonula occludens (ZO-1), was analyzed using quantitative real-time polymerase chain reaction (qRT\u2013PCR) and western blotting. The membrane permeability of GV230\/HIF-1\u03b1-transfected or shRNA-HIF-1\u03b1-transfected RAECs, as well as the expression levels of HIF-1\u03b1, ET-1, ETA, ETB, vascular endothelial (VE)-cadherin, and claudin-5, was analyzed using qRT\u2013PCR and western blotting, whereas the localization of VE-cadherin and claudin-5 was examined using immunofluorescence. The serum HIF-1\u03b1 and ET-1 levels in the burn groups, which peaked at 12 hours postburn, were significantly upregulated (P &amp;lt; .01) when compared with those in the control group. Additionally, the serum HIF-1\u03b1 levels were positively correlated with vascular permeability. Compared with the shRNA-negative control-transfected RAECs, the shRNA-II\/HIF-1\u03b1-transfected RAECs exhibited downregulated expression of HIF-1\u03b1, ET-1, ETA, and ETB (P &amp;lt; .01), and upregulated expression of ZO-1, claudin-5, and VE-cadherin (P &amp;lt; .05). Compared with the GV230-transfected RAECs, the GV230\/HIF-1\u03b1-transfected RAECs exhibited upregulated expression of HIF-1\u03b1, ET-1, ETA, and ETB (P &amp;lt; .01), and downregulated expression of ZO-1, claudin-5, and VE-cadherin (P &amp;lt; .05). The GV230\/HIF-1\u03b1-transfected RAECs exhibited degradation and translocation of VE-cadherin and claudin-5. In addition to degradation of VE-cadherin and claudin-5, HIF-1\u03b1 mediated enhanced endothelial cell permeability through upregulation of ET-1, ETA, and ETB, and downregulation of ZO-1 and VE-cadherin in rats with burn injury.<\/jats:p>","DOI":"10.1093\/jbcr\/iraa139","type":"journal-article","created":{"date-parts":[[2020,8,25]],"date-time":"2020-08-25T10:07:08Z","timestamp":1598350028000},"page":"258-268","source":"Crossref","is-referenced-by-count":11,"title":["HIF-1\u03b1 Enhances Vascular Endothelial Cell Permeability Through Degradation and Translocation of Vascular Endothelial Cadherin and Claudin-5 in Rats With Burn Injury"],"prefix":"10.1093","volume":"42","author":[{"given":"Wei","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Burn, The First Affiliated Hospital of Anhui Medical University Department of Burn, The First Affiliated Hospital of Anhui Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youjun","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Burn, The First Affiliated Hospital of Anhui Medical University Department of Burn, The First Affiliated Hospital of Anhui Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Burn, The First Affiliated Hospital of Anhui Medical University Department of Burn, The First Affiliated Hospital of Anhui Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zijian","family":"Hu","sequence":"additional","affiliation":[{"name":"2018 Class of Clinical Medicine (No. 1813010207), The First Clinical College of Anhui Medical University, Hefei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhui","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Burn, The First Affiliated Hospital of Anhui Medical University Department of Burn, The First Affiliated Hospital of Anhui Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengying","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Burn, The First Affiliated Hospital of Anhui Medical University Department of Burn, The First Affiliated Hospital of Anhui Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengliang","family":"Duan","sequence":"additional","affiliation":[{"name":"Department of Burn, The First Affiliated Hospital of Anhui Medical University Department of Burn, The First Affiliated Hospital of Anhui Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyong","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Burn, The First Affiliated Hospital of Anhui Medical University Department of Burn, The First Affiliated Hospital of Anhui Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youxin","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Burn, The First Affiliated Hospital of Anhui Medical University Department of Burn, The First Affiliated Hospital of Anhui Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delin","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Burn, The First Affiliated Hospital of Anhui Medical University Department of Burn, The First Affiliated Hospital of Anhui Medical University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,8,25]]},"reference":[{"key":"2021030904540644000_CIT0001","first-page":"62","article-title":"Alteration of vascular permeability in burn injury","volume":"1","author":"Huang","year":"2014","journal-title":"Medical 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