{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T23:47:44Z","timestamp":1792108064740,"version":"4.4.0"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T00:00:00Z","timestamp":1710288000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Science and Technology Action Innovation Plan of Shanghai","award":["23QB1401000"],"award-info":[{"award-number":["23QB1401000"]}]},{"name":"Young Innovative Talents Project of Zhejiang Health Science and Technology Plan","award":["2022RC237"],"award-info":[{"award-number":["2022RC237"]}]},{"name":"Medical and Health Science and Technology Project of Hangzhou","award":["B20200432"],"award-info":[{"award-number":["B20200432"]}]},{"name":"Special Policy for Shanghai University Science and Technology Park\u2014Science and Technology Innovation Special Fund","award":["2022-SYXM-002"],"award-info":[{"award-number":["2022-SYXM-002"]}]},{"name":"Special Fund for Promoting High Quality Development in Shanghai\u2014Key Fields of Industrial Strategy Technology Research\u2014Biomedical Special Topic","award":["SY2022008"],"award-info":[{"award-number":["SY2022008"]}]},{"name":"Hangzhou Science and Technology Development Plan","award":["20210133X05"],"award-info":[{"award-number":["20210133X05"]}]},{"name":"Military Medical Science and Technology Youth Cultivation Program","award":["18QNP050"],"award-info":[{"award-number":["18QNP050"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Hypertrophic scar development is a complication associated with wound healing, impacting local appearance and function. The type I\/III collagen ratio affects the extent of hypertrophic scarring; a reduced ratio can ameliorate this. In this study, recombinant human collagen type III was developed. Liquid chromatography\u2013tandem mass spectrometry was used to determine its amino acid sequence and confirm its high level of homology with natural human type III collagen. Recombinant human collagen type III displayed no cytotoxicity and did not confer skin irritation and sensitization. Immunofluorescence and western blot analyses of histidine following incubation with fibroblasts suggested cell entry of recombinant human collagen type III. Furthermore, recombinant human collagen type III promoted the synthesis of the natural type III collagen in fibroblasts, resulting in a more obvious increase of type III collagen content in fibroblasts than that of type I collagen, and then decreased the ratio of type I\/III collagen. The results of 5-ethynyl-2\u02b9-deoxyuridine staining assay suggested enhanced fibroblast proliferation. Following local injection of recombinant human collagen type III, rabbit ear scarring was significantly reduced after 60 days. Vancouver Scar Scale evaluation showed that all index scores were significantly reduced. Western blotting and Picro-Sirius red staining showed that the natural type III collagen increase in scar tissue was greater than that of type I collagen, decreasing the type I\/III ratio. In summary, recombinant human collagen type III can be taken up by fibroblasts and promote natural collagen synthesis\u2014especially that of type III\u2014thereby reducing the type I\/III ratio and improving hypertrophic scarring.<\/jats:p>","DOI":"10.1093\/jbcr\/irae040","type":"journal-article","created":{"date-parts":[[2024,3,12]],"date-time":"2024-03-12T16:38:15Z","timestamp":1710261495000},"page":"1269-1273","source":"Crossref","is-referenced-by-count":36,"title":["Recombinant Human Collagen Type III Improves Hypertrophic Scarring by Regulating the Ratio of Type I\/III Collagen"],"prefix":"10.1093","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4269-3944","authenticated-orcid":false,"given":"Li","family":"Lin-Hui","sequence":"first","affiliation":[{"name":"Department of Burns, The First Affiliated Hospital of Naval Medical University , Shanghai 200433 , 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