{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T09:20:36Z","timestamp":1762680036375,"version":"build-2065373602"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T00:00:00Z","timestamp":1747872000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000005","name":"Department of Defense","doi-asserted-by":"publisher","award":["W81XWH-19-2-0061"],"award-info":[{"award-number":["W81XWH-19-2-0061"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Severe burn injuries are associated with endothelial, coagulopathic, and inflammatory dysfunction, requiring resuscitation; this is most often performed with intravenous crystalloids. Colloids, like fresh frozen plasma (FFP), can be used as adjuncts. This prospective observational cohort study investigated whether the varying factor and protein concentrations in a unit of FFP, as well as its allogenic nature, affected the preexisting prothrombotic, fibrinolytic, and hyperinflammatory state in burn shock in a group of patients with \u226520% total body surface area (TBSA) who receive transfused plasma as part of the standard of care for resuscitation at a regional burn center. Blood samples were collected immediately prior to administration of the first unit of FFP (Pre-Unit) and immediately after administration of the first unit of FFP (Post-Unit). Concentrations of 31 plasma biomarkers were quantified and compared using a paired t-test or a Wilcoxon signed-rank test. Data presented as median (interquartile range). Of the 33 patients included, the median TBSA burn was 34.0% (27.3-45.5). Post-Unit plasma concentrations were decreased compared to Pre-Unit concentrations in factor VIII (329 [201.9-474.1] vs 277 [189.5-438.1] %), fibrinogen (322 [295.3-412.8] vs 305 [285.3-388.5] mg\/dL), protein C (105 [96.0-111.5] vs 102 [91.5-112.0] %), total tissue factor pathway inhibitor (TFPI) (69.7 [51.1-96.3] vs 67.7 [50.9-84.5] ng\/mL), free TFPI (18.2 [14.2-21.8] vs 16.8 [12.9-21.1] ng\/mL), activated\/inactivated thrombin-activatable fibrinolysis inhibitor (55.1 [40.7-80.4] vs 48.7 [38.4-64.4] ng\/mL), C1 esterase inhibitor (149.6 [132.3-222.1] vs 119.6 [110.4-151.6] %), and TNF-\u03b1 receptor 1 (2010 [1451-3535] vs 1815 [1418-3189] pg\/mL) (P \u2264 .05). The 23 other biomarkers did not change. While there were small but statistically significant reductions in 8 markers of coagulation, antifibrinolysis, and inflammation, the Post-Unit concentrations either remained within or moved closer to the expected reference ranges. After the initial transfusion of FFP, there were no clinically significant changes in the preexisting prothrombotic, fibrinolytic, endothelial, or inflammatory biological profile.<\/jats:p>","DOI":"10.1093\/jbcr\/iraf092","type":"journal-article","created":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T08:15:07Z","timestamp":1747383307000},"page":"1194-1200","source":"Crossref","is-referenced-by-count":0,"title":["Characterization of the Endothelial, Coagulofibrinolytic, and Inflammatory Profile in Burn Patients after Resuscitation with Fresh Frozen Plasma"],"prefix":"10.1093","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-8332-5293","authenticated-orcid":false,"given":"Amanda M","family":"Soo Ping Chow","sequence":"first","affiliation":[{"name":"Firefighters\u2019 Burn and Surgical Research Laboratory, MedStar Health Research Institute , Washington, DC ,","place":["United States"]},{"name":"The Burn Center, Department of Surgery, MedStar Washington Hospital Center , Washington, DC ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony E","family":"Pusateri","sequence":"additional","affiliation":[{"name":"59th Medical Wing, JBSA-Lackland Air Force Base, \u00a0 San Antonio, TX ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1539-6346","authenticated-orcid":false,"given":"Tuan D","family":"Le","sequence":"additional","affiliation":[{"name":"Firefighters\u2019 Burn and Surgical Research Laboratory, MedStar Health Research Institute , Washington, DC ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Orfeo","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Larner College of Medicine, University of Vermont , Colchester, VT ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew","family":"Gissel","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Larner College of Medicine, University of Vermont , Colchester, VT ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3270-7217","authenticated-orcid":false,"given":"Maria Cristina","family":"Bravo","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Larner College of Medicine, University of Vermont , Colchester, VT ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melissa","family":"McLawhorn","sequence":"additional","affiliation":[{"name":"Firefighters\u2019 Burn and Surgical Research Laboratory, MedStar Health Research Institute , Washington, DC ,","place":["United States"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauren T","family":"Moffatt","sequence":"additional","affiliation":[{"name":"Firefighters\u2019 Burn and Surgical Research Laboratory, MedStar Health Research Institute , Washington, DC ,","place":["United States"]},{"name":"Department of Surgery, Georgetown University School of Medicine , Washington, DC ,","place":["USA"]},{"name":"Department of Biochemistry and Molecular & Cellular Biology, Georgetown University School of Medicine , Washington, DC ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4394-6416","authenticated-orcid":false,"given":"Jeffrey W","family":"Shupp","sequence":"additional","affiliation":[{"name":"Firefighters\u2019 Burn and Surgical Research Laboratory, MedStar Health Research Institute , Washington, DC ,","place":["United States"]},{"name":"The Burn Center, Department of Surgery, MedStar Washington Hospital Center , Washington, DC ,","place":["United States"]},{"name":"Department of Surgery, Georgetown University School of Medicine , Washington, DC ,","place":["USA"]},{"name":"Department of Biochemistry and Molecular & Cellular Biology, Georgetown University School of Medicine , Washington, DC ,","place":["USA"]},{"name":"Department of Plastic and Reconstructive Surgery, Georgetown University School of Medicine , Washington, DC ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,5,22]]},"reference":[{"key":"2025110904192745900_CIT0001","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/j.suc.2023.02.002","article-title":"The first 24 hours: burn shock resuscitation and early complications","volume":"103","author":"Tejiram","year":"2023","journal-title":"Surg Clin North Am"},{"key":"2025110904192745900_CIT0002","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1097\/SHK.0000000000002473","article-title":"Fibrinolytic dysfunction and endotheliopathy after major thermal injury: 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