{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:06:15Z","timestamp":1792785975265,"version":"4.5.0"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"2","funder":[{"name":"Intramural Research programs"},{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Bone histomorphometry is a well-established approach to assessing skeletal pathology, providing a standard evaluation of the cellular components, architecture, mineralization, and growth of bone tissue. However, it depends in part on the subjective interpretation of cellular morphology by an expert, which introduces bias. In addition, diseases like osteogenesis imperfecta (OI) and fibrous dysplasia are accompanied by changes in the morphology and function of skeletal tissue and cells, hindering consistent evaluation of some morphometric parameters and interpretation of the results. For instance, traditional histomorphometry combined with collagen turnover markers suggested that reduced bone formation in classical OI is accompanied by increased bone resorption. In contrast, the well-documented postpubertal reduction in fractures would be easier to explain by reduced bone resorption after puberty, highlighting the need for less ambiguous measurements. Here we propose an approach to histomorphometry based on in situ mRNA hybridization, which uses Col1a1 as osteoblast and Ctsk as osteoclast markers. This approach can be fully automated and eliminates subjective identification of bone surface cells. We validate these markers based on the expression of Bglap, Ibsp, and Acp5. Comparison with traditional histological and tartrate-resistant acid phosphatase staining of the same sections suggests that mRNA-based analysis is more reliable. Unlike inconclusive traditional histomorphometry of mice with \u03b12(I)-Gly610 to Cys substitution in the collagen triple helix, mRNA-based measurements reveal reduced osteoclastogenesis in 11-wk-old animals consistent with the postpubertal catch-up osteogenesis observed by microCT. We optimize the technique for cryosections of mineralized bone and sections of paraffin-embedded decalcified tissue, simplifying and broadening its applications. We illustrate the application of the mRNA-based approach to human samples using the example of a McCune-Albright syndrome patient. By eliminating confounding effects of altered cellular morphology and the need for subjective morphological evaluation, this approach may provide a more reproducible and accessible evaluation of bone pathology.<\/jats:p>","DOI":"10.1093\/jbmr\/zjad004","type":"journal-article","created":{"date-parts":[[2024,1,2]],"date-time":"2024-01-02T17:00:41Z","timestamp":1704214841000},"page":"177-189","source":"Crossref","is-referenced-by-count":4,"title":["RNA-based bone histomorphometry: method and its application to explaining postpubertal bone gain in a G610C mouse model of osteogenesis imperfecta"],"prefix":"10.1093","volume":"39","author":[{"given":"Elena","family":"Makareeva","sequence":"first","affiliation":[{"name":"Eunice Kennedy Shriver National institute of Child Health and Human Development, National Institutes of Health , Bethesda, MD 20892 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan","family":"Sousa","sequence":"additional","affiliation":[{"name":"Eunice Kennedy Shriver National institute of Child Health and Human Development, National Institutes of Health , Bethesda, MD 20892 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tristan","family":"Kent","sequence":"additional","affiliation":[{"name":"National Institute of Dental and Craniofacial Research, National Institutes of Health , Bethesda, MD 20892 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3194-9780","authenticated-orcid":false,"given":"Luis F","family":"de Castro","sequence":"additional","affiliation":[{"name":"National Institute of Dental and Craniofacial Research, National Institutes of Health , Bethesda, MD 20892 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael T","family":"Collins","sequence":"additional","affiliation":[{"name":"National Institute of Dental and Craniofacial Research, National Institutes of Health , Bethesda, MD 20892 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7095-0739","authenticated-orcid":false,"given":"Sergey","family":"Leikin","sequence":"additional","affiliation":[{"name":"Eunice Kennedy Shriver National institute of Child Health and Human Development, National Institutes of Health , Bethesda, MD 20892 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,1,4]]},"reference":[{"issue":"2","key":"2024032223040009600_ref1","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/BF02556831","article-title":"Inter-observer and intra-observer variation in bone histomorphometry","volume":"38","author":"Compston","year":"1986","journal-title":"Calcif Tissue 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histomorphometry","volume":"16","author":"Rowe","year":"2018","journal-title":"Curr Osteoporos Rep"},{"issue":"1","key":"2024032223040009600_ref6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00223-022-01035-2","article-title":"Contemporary advances in computer-assisted bone histomorphometry and identification of bone cells in culture","volume":"112","author":"Brent","year":"2023","journal-title":"Calcif Tissue Int"},{"key":"2024032223040009600_ref7","doi-asserted-by":"crossref","DOI":"10.3389\/fendo.2022.907914","article-title":"Interest of bone histomorphometry in bone pathophysiology investigation: foundation, present, and future","volume":"13","author":"Chavassieux","year":"2022","journal-title":"Front Endocrinol"},{"issue":"1","key":"2024032223040009600_ref8","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1002\/jbmr.1805","article-title":"Standardized nomenclature, symbols, and units for bone histomorphometry: a 2012 update of the report of the ASBMR 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