{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:16:17Z","timestamp":1791303377406,"version":"4.1.0"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2024,4,23]],"date-time":"2024-04-23T00:00:00Z","timestamp":1713830400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32200943"],"award-info":[{"award-number":["32200943"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82072387"],"award-info":[{"award-number":["82072387"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018559","name":"Shenyang Young and Middle-aged Science and Technology Innovation Talent Support Program","doi-asserted-by":"publisher","award":["RC210171"],"award-info":[{"award-number":["RC210171"]}],"id":[{"id":"10.13039\/501100018559","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The role of monocytes in postmenopausal osteoporosis is widely recognized; however, the mechanisms underlying monocyte reprogramming remain unknown. In this study, single-cell RNA sequencing (scRNA-seq) was conducted on CD14+ bone marrow monocytes obtained from 3 postmenopausal women with normal BMD and 3 women with postmenopausal osteoporosis (PMOP). Monocle2 was used to classify the monocytes into 7 distinct clusters. The proportion of cluster 1 significantly decreased in PMOP patients, while the proportion of cluster 7 increased. Further analysis via GSEA, transcription factor activity analysis, and sc-metabolic analysis revealed significant differences between clusters 1 and 7. Cluster 7 exhibited upregulated pathways associated with inflammation, immunity, and osteoclast differentiation, whereas cluster 1 demonstrated the opposite results. Monocle2, TSCAN, VECTOR, and scVelo data indicated that cluster 1 represented the initial subset and that cluster 7 represents one of the terminal subsets. BayesPrism and ssGSEA were employed to analyze the bulk transcriptome data obtained from the GEO database. The observed alterations in the proportions of 1 and 7 were validated and found to have diagnostic significance. CD16 serves as the marker gene for cluster 7, thus leading to an increased proportion of CD16+ monocytes in women with PMOP. Flow cytometry was used to assess the consistency of outcomes with those of the bioinformatic analysis. Subsequently, an additional scRNA-seq analysis was conducted on bone marrow mononuclear cells obtained from 3 patients with PMOP and 3 postmenopausal women with normal BMD. The differential proportions of cluster 1 and cluster 7 were once again confirmed, with the pathological effect of cluster 7 may attribute to cell\u2013cell communication. The scRNA-seq findings suggest that an imbalance in monocyte subsets is a characteristic feature of PMOP. These findings elucidate the limitations of utilizing bulk transcriptome data for detecting alterations in monocytes, which may influence novel research inquiries.<\/jats:p>","DOI":"10.1093\/jbmr\/zjae065","type":"journal-article","created":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T13:32:44Z","timestamp":1713792764000},"page":"980-993","source":"Crossref","is-referenced-by-count":24,"title":["Single-cell RNA sequencing reveals that an imbalance in monocyte subsets rather than changes in gene expression patterns is a feature of postmenopausal osteoporosis"],"prefix":"10.1093","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1702-9833","authenticated-orcid":false,"given":"Lin","family":"Tao","sequence":"first","affiliation":[{"name":"Department of Orthopedics, First Hospital of China Medical University , Shenyang, Liaoning, 110000 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, First Hospital of China Medical University , Shenyang, Liaoning, 110000 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Shanghai Pudong New Area People\u2019s Hospital , Shanghai, 200000 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaochuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, First Hospital of China Medical University , Shenyang, Liaoning, 110000 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixuan","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, First Hospital of China Medical University , Shenyang, Liaoning, 110000 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3470-0414","authenticated-orcid":false,"given":"Keda","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, First Hospital of China Medical University , Shenyang, Liaoning, 110000 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0126-496X","authenticated-orcid":false,"given":"Yue","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, First Hospital of China Medical University , Shenyang, Liaoning, 110000 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,4,23]]},"reference":[{"issue":"8","key":"2024080610513695100_ref1","doi-asserted-by":"crossref","first-page":"e2121106","DOI":"10.1001\/jamanetworkopen.2021.21106","article-title":"Prevalence of osteoporosis and fracture in China: the China osteoporosis prevalence study","volume":"4","author":"Wang","year":"2021","journal-title":"JAMA Netw Open"},{"issue":"9319","key":"2024080610513695100_ref2","doi-asserted-by":"crossref","first-page":"1761","DOI":"10.1016\/S0140-6736(02)08657-9","article-title":"Epidemiology and outcomes of osteoporotic fractures","volume":"359","author":"Cummings","year":"2002","journal-title":"Lancet"},{"issue":"4","key":"2024080610513695100_ref3","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1002\/jbmr.5650050402","article-title":"Cancellous bone remodeling in type I (postmenopausal) osteoporosis: quantitative assessment of rates of formation, resorption, and bone loss at tissue and cellular levels","volume":"5","author":"Eriksen","year":"1990","journal-title":"J Bone Miner Res"},{"issue":"1","key":"2024080610513695100_ref4","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1186\/ar2110","article-title":"Cells of the synovium in rheumatoid arthritis. 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