{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T17:06:18Z","timestamp":1787591178993,"version":"build-2736575974"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T00:00:00Z","timestamp":1754956800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003554","name":"The Lundbeck Foundation","doi-asserted-by":"crossref","award":["R16-A1694"],"award-info":[{"award-number":["R16-A1694"]}],"id":[{"id":"10.13039\/501100003554","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007037","name":"The Danish Ministry of Health","doi-asserted-by":"crossref","award":["1601148"],"award-info":[{"award-number":["1601148"]}],"id":[{"id":"10.13039\/501100007037","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007398","name":"Danish Council for Strategic Research","doi-asserted-by":"publisher","award":["0603-00280B"],"award-info":[{"award-number":["0603-00280B"]}],"id":[{"id":"10.13039\/100007398","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The Capital Region Research Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The gut microbiome is associated with bone mass acquisition, yet evidence in childhood remains limited. Given that lower peak bone mass predicts osteoporosis in later life, understanding early influences is important. This analysis explores the association between the early life gut microbiome and bone health in later childhood. Data were obtained from 700 children recruited in pregnancy and followed prospectively within the Copenhagen Prospective Studies on Asthma in Childhood2010 cohort, a population-based mother-child cohort. The infant gut microbiome was measured at 1 wk (n\u2009=\u2009445), 1 mo (n\u2009=\u2009492), 1\u00a0yr (n\u2009=\u2009508), 4\u00a0yr (n\u2009=\u2009350), and 6\u00a0yr (n\u2009=\u2009327) of age by 16S ribosomal ribonucleic acid amplicon sequencing targeting the fourth variable region. Total body less head BMD and area-adjusted BMC were measured by DXA at 6\u00a0yr of age. Associations were investigated by multiple linear regression, permutational analysis of variance, differential abundance analysis, and Random Forest machine learning. There were few associations between the early-life gut microbiome and bone health outcomes at age 6. We found negative associations between alpha (within-sample) diversity and area-adjusted BMC at 4\u00a0yr. Beta (between-sample) diversity of the gut microbiome at 6\u00a0yr was associated with concurrent BMD. Escherichia-Shigella abundance at 1 mo of age was associated with lower BMD. Sutterella abundance at 1\u00a0yr was associated with lower BMD and area-adjusted BMC at 6\u00a0yr. There were no other associations between the gut microbiome and bone outcome measures at any time point. In a well-powered unselected cohort study with longitudinal sampling of the gut microbiome, there were some suggestive but no consistent associations between the early gut microbiome and bone health outcomes at 6\u00a0yr of age.<\/jats:p>","DOI":"10.1093\/jbmr\/zjaf108","type":"journal-article","created":{"date-parts":[[2025,8,12]],"date-time":"2025-08-12T11:37:53Z","timestamp":1754998673000},"page":"1278-1289","source":"Crossref","is-referenced-by-count":1,"title":["Gut microbiome in early life and bone health outcomes at age 6: a Danish mother-child cohort study"],"prefix":"10.1093","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7540-7337","authenticated-orcid":false,"given":"Pantalius Nji","family":"Che","sequence":"first","affiliation":[{"name":"Menzies Institute for Medical Studies, University of Tasmania , Hobart, 7000,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Jiang","sequence":"additional","affiliation":[{"name":"Copenhagen Prospective Studies on Asthma in Childhood (COPSAC) , University of Copenhagen, Copenhagen, 2820,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique","family":"Breslin","sequence":"additional","affiliation":[{"name":"Menzies Institute for Medical Studies, University of Tasmania , Hobart, 7000,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1425-5808","authenticated-orcid":false,"given":"Michael","family":"Thompson","sequence":"additional","affiliation":[{"name":"Menzies Institute for Medical Studies, University of Tasmania , Hobart, 7000,","place":["Australia"]},{"name":"Department of Endocrinology, Royal Hobart Hospital , Hobart, 7000, TAS ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca K","family":"Vinding","sequence":"additional","affiliation":[{"name":"Copenhagen Prospective Studies on Asthma in Childhood (COPSAC) , University of Copenhagen, Copenhagen, 2820,","place":["Denmark"]},{"name":"Department of Pediatrics and Adolescent Medicine, Copenhagen University Hospital \u2212 Herlev and Gentofte , Herlev , 2730,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakob","family":"Stokholm","sequence":"additional","affiliation":[{"name":"Copenhagen Prospective Studies on Asthma in Childhood (COPSAC) , University of Copenhagen, Copenhagen, 2820,","place":["Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lieke E J M","family":"Scheepers","sequence":"additional","affiliation":[{"name":"Menzies Institute for Medical Studies, University of Tasmania , Hobart, 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