{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:29:18Z","timestamp":1792794558431,"version":"4.5.0"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2024,1,28]],"date-time":"2024-01-28T00:00:00Z","timestamp":1706400000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["165969"],"award-info":[{"award-number":["165969"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["133413"],"award-info":[{"award-number":["133413"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Dean of Medicine and Discipline of Medicine"},{"name":"Memorial University","id":[{"id":"https:\/\/ror.org\/04haebc03","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Calcitriol circulates at low levels in normal human and rodent fetuses, in part due to increased 24-hydroxylation of calcitriol and 25-hydroxyvitamin D by 24-hydroxylase (CYP24A1). Inactivating mutations of CYP24A1 cause high postnatal levels of calcitriol and the human condition of infantile hypercalcemia type 1, but whether the fetus is disturbed by the loss of CYP24A1 is unknown. We hypothesized that loss of Cyp24a1 in fetal mice will cause high calcitriol, hypercalcemia, and increased placental calcium transport. The Cyp24a1+\/\u2212 mice were mated to create pregnancies with wildtype, Cyp24a1+\/\u2212, and Cyp24a1 null fetuses. The null fetuses were hypercalcemic, modestly hypophosphatemic (compared to Cyp24a1+\/\u2212 fetuses only), with 3.5-fold increased calcitriol, 4-fold increased fibroblast growth factor 23 (FGF23), and unchanged parathyroid hormone. The quantitative RT-PCR confirmed the absence of Cyp24a1 and 2-fold increases in S100g, sodium\u2013calcium exchanger type 1, and calcium-sensing receptor in null placentas but not in fetal kidneys; these changes predicted an increase in placental calcium transport. However, placental 45Ca and 32P transport were unchanged in null fetuses. Fetal ash weight and mineral content, placental weight, crown-rump length, and skeletal morphology did not differ among the genotypes. Serum procollagen 1 intact N-terminal propeptide and bone expression of sclerostin and Blgap were reduced while calcitonin receptor was increased in nulls. In conclusion, loss of Cyp24a1 in fetal mice causes hypercalcemia, modest hypophosphatemia, and increased FGF23, but no alteration in skeletal development. Reduced incorporation of calcium into bone may contribute to the hypercalcemia without causing a detectable decrease in the skeletal mineral content. The results predict that human fetuses bearing homozygous or compound heterozygous inactivating mutations of CYP24A1 will also be hypercalcemic in utero but with normal skeletal development.<\/jats:p>","DOI":"10.1093\/jbmrpl\/ziae012","type":"journal-article","created":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T15:33:19Z","timestamp":1706023999000},"source":"Crossref","is-referenced-by-count":8,"title":["Loss of 24-hydroxylated catabolism increases calcitriol and fibroblast growth factor 23 and alters calcium and phosphate metabolism in fetal mice"],"prefix":"10.1093","volume":"8","author":[{"given":"David","family":"Bennin","sequence":"first","affiliation":[{"name":"Faculty of Medicine \u2013 Endocrinology, Memorial University of Newfoundland , St. John\u2019s, Newfoundland and Labrador, A1B 3V6 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah A","family":"Hartery","sequence":"additional","affiliation":[{"name":"Faculty of Medicine \u2013 Endocrinology, Memorial University of Newfoundland , St. John\u2019s, Newfoundland and Labrador, A1B 3V6 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beth J","family":"Kirby","sequence":"additional","affiliation":[{"name":"Faculty of Medicine \u2013 Endocrinology, Memorial University of Newfoundland , St. John\u2019s, Newfoundland and Labrador, A1B 3V6 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre S","family":"Maekawa","sequence":"additional","affiliation":[{"name":"Faculty of Medicine \u2013 Endocrinology, Memorial University of Newfoundland , St. John\u2019s, Newfoundland and Labrador, A1B 3V6 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9","family":"St-Arnaud","sequence":"additional","affiliation":[{"name":"Shriners Hospitals for Children\u2013Canada and McGill University , Montr\u00e9al, Quebec, H4A 0A9 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher S","family":"Kovacs","sequence":"additional","affiliation":[{"name":"Faculty of Medicine \u2013 Endocrinology, Memorial University of Newfoundland , St. John\u2019s, Newfoundland and Labrador, A1B 3V6 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,1,29]]},"reference":[{"key":"2024040411371508100_ref1","first-page":"496","article-title":"Vitamin D endocrine system and the intestine","volume":"3","author":"Christakos","year":"2014","journal-title":"BoneKEy Rep Rev"},{"issue":"4","key":"2024040411371508100_ref2","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1152\/physrev.00014.2014","article-title":"Bone development and mineral homeostasis in the fetus and neonate: roles of the calciotropic and phosphotropic hormones","volume":"94","author":"Kovacs","year":"2014","journal-title":"Physiol Rev"},{"key":"2024040411371508100_ref3","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1016\/B978-0-12-381978-9.10064-2","volume-title":"Vitamin D","author":"Glorieux","year":"2011","edition":"3rd edn"},{"key":"2024040411371508100_ref4","first-page":"524","article-title":"Vitamin D\/dietary calcium deficiency rickets and pseudo-vitamin D deficiency rickets","volume":"3","author":"Glorieux","year":"2014","journal-title":"BoneKEy Rep. 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