{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T09:46:47Z","timestamp":1794476807598,"version":"4.9.1"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2024,3,4]],"date-time":"2024-03-04T00:00:00Z","timestamp":1709510400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MinnCResT Fellowship","award":["T90 DE022732\/R90 DE023058"],"award-info":[{"award-number":["T90 DE022732\/R90 DE023058"]}]},{"name":"Osteoscience Foundation Peter Geistlich Research Award"},{"name":"University of Minnesota School of Dentistry Summer Research Fellowship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Craniofacial osteoclasts are essential for site\u2013specific processes such as alveolar bone resorption, tooth eruption, and orthodontic tooth movement. Much of the current understanding of osteoclast development and function comes from studies using long bone\u2013derived cells. Minimal investigation has been done to explore skeletal site differences. The overall goal of this study was to determine if mandibular\u2013 and femoral\u2013derived osteoclasts represent distinct populations. To test this hypothesis, bone marrow cells were initially analyzed from the mandible and femur of 2\u2013month\u2013old mice. It was shown that mandibular\u2013derived osteoclasts have enhanced size (mm2) compared with femoral\u2013derived osteoclasts. Since bone marrow macrophages are a heterogenous population, we additionally selected for monocytes and demonstrated that mandibular\u2013derived monocytes also form osteoclasts with increased size compared with femoral\u2013derived monocytes. Osteoclast precursor populations from both skeletal sites were analyzed by flow cytometry. A newly described Ly6CHigh+ population as well as the Ly6Cint population was increased in the mandibular\u2013derived cells. The difference in differentiation potential between monocyte cultures suggests that the increase in the Ly6CHigh+ population may explain the enhanced differentiation potential in mandibular\u2013derived cells. Monocyte genes such as Pu.1, C\/ebp-a, and Prdm1 are increased in expression in mandibular\u2013derived monocytes compared with femoral\u2013derived monocytes. As expected with enhanced differentiation, osteoclast genes including Nfatc1, Dc-stamp, Ctsk, and Rank are upregulated in mandibular\u2013derived osteoclast precursors. Future studies will determine how changes in the environment of the mandible lead to changes in percentages of osteoclast progenitors and their differentiation potential.<\/jats:p>","DOI":"10.1093\/jbmrpl\/ziae029","type":"journal-article","created":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T01:24:11Z","timestamp":1709601851000},"source":"Crossref","is-referenced-by-count":9,"title":["Mouse mandibular\u2013derived osteoclast progenitors have differences in intrinsic properties compared with femoral\u2013derived progenitors"],"prefix":"10.1093","volume":"8","author":[{"given":"Rachel","family":"Clark","sequence":"first","affiliation":[{"name":"Department of Developmental and Surgical Sciences , Oral Biology Graduate Program, , Minneapolis, MN 55455 , United States"},{"name":"University of Minnesota School of Dentistry , Oral Biology Graduate Program, , Minneapolis, MN 55455 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soo Y","family":"Park","sequence":"additional","affiliation":[{"name":"School of Dentistry, University of Minnesota , Minneapolis, MN 55455 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth W","family":"Bradley","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, University of Minnesota , Minneapolis, MN 55455 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim","family":"Mansky","sequence":"additional","affiliation":[{"name":"Division of Orthodontics , Department of Developmental and Surgical Sciences, , Minneapolis, MN 55455 , United States"},{"name":"University of Minnesota School of Dentistry Minneapolis , Department of Developmental and Surgical Sciences, , Minneapolis, MN 55455 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Tasca","sequence":"additional","affiliation":[{"name":"Division of Orthodontics , Department of Developmental 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