{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T23:44:26Z","timestamp":1793490266504,"version":"4.5.0"},"reference-count":60,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:00:00Z","timestamp":1718668800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon 2020 Research and Innovation Programme","award":["766347"],"award-info":[{"award-number":["766347"]}]},{"DOI":"10.13039\/501100004385","name":"Ghent University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004385","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Bijzonder Onderzoeksfonds","award":["BOF.ITN.2021.0012.01"],"award-info":[{"award-number":["BOF.ITN.2021.0012.01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,6,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Bone matrix formation and mineralization are two closely related, yet separated processes. Matrix formation occurs first, mineralization is a second step strictly dependent on the dietary intake of calcium and phosphorus (P). However, mineralization is commonly used as diagnostic parameter for bone-related diseases. In this context, bone loss, often characterized as a condition with reduced bone mineral density, represents a major burden for human health, for which increased dietary mineral intake is generally recommended. Using a counterintuitive approach, we use a low-P diet followed by a sufficient-P intake to increase bone volume. We show in zebrafish by histology, qPCR, micro-CT, and enzyme histochemistry that a two-months period of reduced dietary P intake stimulates extensive formation of new bone matrix, associated with the upregulation of key genes required for both bone matrix formation and mineralization. The return to a P-sufficient diet initiates the mineralization of the abundant matrix previously deposited, thus resulting in a striking increase of the mineralized bone volume as proven at the level of the vertebral column, including vertebral bodies and arches. In summary, bone matrix formation is first stimulated with a low-P diet, and its mineralization is later triggered by a sufficient-P dietary intake. In zebrafish, the uncoupling of bone formation and mineralization by alternating low and sufficient dietary P intake significantly increases the bone volume without causing skeletal malformations or ectopic mineralization. A modification of this approach to stimulate bone formation, optimized for mammalian models, can possibly open opportunities to support treatments in patients that suffer from low bone mass.<\/jats:p>","DOI":"10.1093\/jbmrpl\/ziae081","type":"journal-article","created":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T05:22:25Z","timestamp":1718688145000},"source":"Crossref","is-referenced-by-count":10,"title":["Matrix first, minerals later: fine-tuned dietary phosphate increases bone formation in zebrafish"],"prefix":"10.1093","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5143-5563","authenticated-orcid":false,"given":"Silvia","family":"Cotti","sequence":"first","affiliation":[{"name":"Biology Department , Evolutionary Developmental Biology, , 9000 Gent , Belgium"},{"name":"Ghent University , Evolutionary Developmental Biology, , 9000 Gent , Belgium"},{"name":"Department of Molecular Medicine , Biochemistry Unit, , 27100 Pavia , Italy"},{"name":"University of Pavia , Biochemistry Unit, , 27100 Pavia , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9096-3166","authenticated-orcid":false,"given":"Claudia","family":"Di Biagio","sequence":"additional","affiliation":[{"name":"Biology Department , Evolutionary Developmental Biology, , 9000 Gent , Belgium"},{"name":"Ghent University , Evolutionary Developmental Biology, , 9000 Gent , Belgium"},{"name":"Department of Biology, University of Rome Tor Vergata , 00133 Rome , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8713-0462","authenticated-orcid":false,"given":"Ann","family":"Huysseune","sequence":"additional","affiliation":[{"name":"Biology Department , Evolutionary Developmental Biology, , 9000 Gent , Belgium"},{"name":"Ghent University , Evolutionary Developmental Biology, , 9000 Gent , Belgium"},{"name":"Department of Zoology, Charles University , 12800 Prague , Czech 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