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They typically affect young people, significantly reducing their quality of life and increasing mortality rates. Giant cell tumors of bone are composed of osteoclast-like giant cells that respond to increased secretion of RANKL by stromal cells, triggering osteolysis. For over a decade, denosumab, a monoclonal antibody targeting this receptor activator, has been approved as a neo-adjuvant to facilitate surgical resection or in the setting of inoperable tumors. Denosumab treatment has shown rapid pain improvement and tumor size reduction in the spine. Although variable degrees of tumor mineralization have been observed in clinical applications of this drug, the nature of this newly formed mineralized tissue has yet to be determined. To characterize both mineralization and collagen organization in the newly formed bone, we conducted extensive analyses on 4 posttreatment giant cell tumor vertebral samples, involving quantitative backscattered imaging, electron probe microanalysis, and a novel method for determining the alignment of collagen fibrils using second harmonic generation. Additionally, biological mechanisms involved in bone mineralization and matrix formation were analyzed using histological staining and mass spectroscopy. Our results concluded that denosumab treatment after giant cell tumor of bone in the spine was associated with the formation of woven bone and increased mineral density in a matrix of disorganized collagen fibers characterized by increased collagen III content, with the response appearing to depend on patient age and extension of treatment. To our knowledge, this is the first comprehensive material-based study on the bone formed during denosumab treatment for GCTB, providing valuable information on how denosumab affects bone quality and how the reported methodology can be applied to similar studies.<\/jats:p>","DOI":"10.1093\/jbmrpl\/ziaf063","type":"journal-article","created":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T14:31:17Z","timestamp":1745418677000},"source":"Crossref","is-referenced-by-count":4,"title":["Denosumab treatment of giant cell tumors in the spine induces woven bone formation"],"prefix":"10.1093","volume":"9","author":[{"given":"Robyn","family":"Birch","sequence":"first","affiliation":[{"name":"School of Biomedical Engineering, University of British Columbia , Vancouver, BC V6T 1Z3 ,","place":["Canada"]},{"name":"Centre for Aging SMART , Vancouver, BC V5Z 1M9 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Eltit","sequence":"additional","affiliation":[{"name":"Vancouver Prostate Centre , Vancouver, BC V6H 3Z6 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis","family":"Xie","sequence":"additional","affiliation":[{"name":"Vancouver Prostate Centre , Vancouver, BC V6H 3Z6 ,","place":["Canada"]},{"name":"Department of Urological Sciences, University of British Columbia , Vancouver, BC V5Z 1M9 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering, University of British Columbia , Vancouver, BC V6T 1Z3 ,","place":["Canada"]},{"name":"Department of Materials Engineering, University of British Columbia , Vancouver, BC V6T 1Z4 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Dea","sequence":"additional","affiliation":[{"name":"Combined Neurosurgical and Orthopedic Spine Program, Department of Orthopedics Surgery, University of British Columbia , Vancouver, BC V5Z 1M9 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles G","family":"Fisher","sequence":"additional","affiliation":[{"name":"Combined Neurosurgical and Orthopedic Spine Program, Department of Orthopedics Surgery, University of British Columbia , Vancouver, BC V5Z 1M9 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael E","family":"Cox","sequence":"additional","affiliation":[{"name":"Centre for Aging SMART , Vancouver, BC V5Z 1M9 ,","place":["Canada"]},{"name":"Vancouver Prostate Centre , Vancouver, BC V6H 3Z6 ,","place":["Canada"]},{"name":"Department of Urological Sciences, University of British Columbia , Vancouver, BC V5Z 1M9 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tony","family":"Ng","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of British Columbia , Vancouver, BC V6T 2B5 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rapha\u00eble","family":"Charest-Morin","sequence":"additional","affiliation":[{"name":"Combined Neurosurgical and Orthopedic Spine Program, Department of Orthopedics Surgery, University of British Columbia , Vancouver, BC V5Z 1M9 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rizhi","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering, University of British Columbia , Vancouver, BC V6T 1Z3 ,","place":["Canada"]},{"name":"Centre for Aging SMART , Vancouver, BC V5Z 1M9 ,","place":["Canada"]},{"name":"Department of Materials Engineering, University of British Columbia , Vancouver, BC V6T 1Z4 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,4,23]]},"reference":[{"issue":"1","key":"2025052713275868900_ref1","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1007\/s00590-019-02554-9","article-title":"Can denosumab cure giant cell tumors of the spine? 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