{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:04:53Z","timestamp":1790899493708,"version":"4.1.0"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T00:00:00Z","timestamp":1768521600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>To investigate the dose-dependent effects of neonatal-onset phosphate deficiency on bone growth and mineralization and assess whether supraphysiological vitamin D3 or calcitriol can rescue skeletal defects. Newborn Sprague-Dawley rats were randomly assigned to 7 diets: phosphate-free (0P), low phosphate (1\/2P), normal phosphate (NP), calcium-free (0Ca), phosphate\/vitamin D-free (0P\/D), and 0P\/D supplemented with either supraphysiological vitamin D3 (0P\/D\u2009+\u2009D3) or calcitriol (0P\/D\u2009+\u2009calcitriol). Longitudinal radiographic assessments were performed before euthanasia at 6-8\u00a0wk. Serum analyses measured phosphate (sP), calcium (sCa), phosphotropic hormones, and bone turnover markers. Tibial growth plates were examined by H&amp;E staining, micro-CT, and histomorphometry. The 0P group developed severe hypophosphatemia, rickets-like growth plate widening, osteopenia, and growth retardation. The 1\/2P group showed similar hypophosphatemia but no growth impairment and non-significant reductions in bone mass. The 0Ca group exhibited hypocalcemia, secondary hyperparathyroidism, and high bone resorption, yet maintained normal growth and intermediate mineralization. Vitamin D interventions normalized sP but worsened bone loss and growth impairment compared to the 0P group. Biochemically, sP correlated positively with bone formation markers and negatively with fibroblast growth factor-23 (FGF23); vitamin D showed dual effects on bone turnover. Phosphate sufficiency during the early postnatal period is critical for bone mineralization and growth in neonatal rats. Isolated calcium deficiency caused a distinct osteomalacic phenotype with preserved growth. Supraphysiological-dose vitamin D metabolites corrected hypophosphatemia but failed to rescue\u2014and may have exacerbated\u2014skeletal defects, cautioning against vitamin D monotherapy without concurrent phosphate supplementation.<\/jats:p>","DOI":"10.1093\/jbmrpl\/ziag007","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T12:43:15Z","timestamp":1768394595000},"source":"Crossref","is-referenced-by-count":2,"title":["Effects of graded phosphate deficiency and vitamin D intervention on growth, bone metabolism, and mineralization in a rat model of neonatal-onset metabolic bone disease"],"prefix":"10.1093","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5470-9586","authenticated-orcid":false,"given":"Shanshan","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Neonatology, Hebei Key Laboratory of Early Life Health Promotion (SZX202419), The Second Hospital of Hebei Medical University, Hebei Medical University , Shijiazhuang, 050000, Hebei Province ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huifeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Hebei Key Laboratory of Early Life Health Promotion (SZX202419), The Second Hospital of Hebei Medical University, Hebei Medical University , Shijiazhuang, 050000, Hebei Province ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,1,16]]},"reference":[{"issue":"1","key":"2026022501312925100_ref1","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1159\/000536228","article-title":"Invited mini review metabolic bone disease of prematurity: overview and practice recommendations","volume":"98","author":"Grover","year":"2025","journal-title":"Horm Res 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