{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T20:08:03Z","timestamp":1789416483119,"version":"build-2803163510"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T00:00:00Z","timestamp":1786147200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100017074","name":"Zinpro Corporation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017074","id-type":"DOI","asserted-by":"publisher"}]},{"name":"DSM Nutritional Products North America"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Fibroblast growth factor 23 (FGF23), a bone-derived hormone, regulates phosphorus (P) homeostasis by modulation of renal P excretion and vitamin D metabolism. Physiological roles of FGF23 have been mainly characterized in human disorders of P metabolism and murine models. This study aimed to validate roles of FGF23 in P homeostasis in a large animal, healthy swine model. Juvenile pigs fed a low-P diet (LP) for 4\u00a0d were fasted overnight then fed a high-P diet (HP) for 5\u00a0d. Blood was collected to determine plasma concentrations of P, calcium (Ca), PTH, and vitamin D metabolites, and urine was collected to assess P and Ca concentrations. Femur and kidney tissues were collected to assess gene and protein expression related to FGF23 synthesis, signaling, and vitamin D metabolism. The rapid increase in plasma P (5 to 18\u00a0mg\/dL) within 12\u00a0h of HP intake was lagged by increased urinary P. At maximal urinary P concentration (108\u00a0h), plasma P had returned to physiological ranges. The initial increase in plasma PTH with HP intake returned to baseline levels by 108\u00a0h. At 108\u00a0h, HP consumption upregulated bone FGF23 mRNA expression (250-fold increase relative to LP). Sodium-phosphate co-transporters (NaPi2a and NaPi2c) mRNA and NaPi2a protein expression decreased with HP intake, consistent with FGF23-mediated phosphaturia, independent of circulating PTH. Bone mRNA expression of post-translational FGF23 regulators (GALNT3 and FURIN) was unaffected by HP consumption, whereas kidney FGF23 receptors (FGFR1 and \u03b1KLOTHO) slightly increased. High P intake downregulated renal 1\u03b1-hydroxylase (CYP27B1) mRNA expression at 108\u00a0h, consistent with decreased circulating 1,25(OH)2D3 concentrations. In conclusion, HP consumption stimulated bone FGF23 expression and renal adaptive responses in healthy pigs, consistent with roles of FGF23-mediated phosphaturia and regulation of vitamin D metabolism for maintenance of P homeostasis, as previously described in humans and rodents.<\/jats:p>","DOI":"10.1093\/jbmrpl\/ziag129","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T11:58:44Z","timestamp":1786017524000},"source":"Crossref","is-referenced-by-count":0,"title":["Bone\n                    <i>FGF23<\/i>\n                    expression and renal regulatory responses to excess phosphate consumption by juvenile swine"],"prefix":"10.1093","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-6282-7718","authenticated-orcid":false,"given":"Mariola","family":"Grez-Capdeville","sequence":"first","affiliation":[{"name":"Department of Animal and Dairy Sciences, University of Wisconsin-Madison , Madison, WI 53706 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6738-8383","authenticated-orcid":false,"given":"Brittney P","family":"Kokinos","sequence":"additional","affiliation":[{"name":"Department of Animal and Dairy Sciences, University of Wisconsin-Madison , Madison, WI 53706 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3461-0486","authenticated-orcid":false,"given":"Laura A","family":"Amundson","sequence":"additional","affiliation":[{"name":"Department of Animal and Dairy Sciences, University of Wisconsin-Madison , Madison, WI 53706 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8886-0264","authenticated-orcid":false,"given":"Thomas D","family":"Crenshaw","sequence":"additional","affiliation":[{"name":"Department of Animal and Dairy Sciences, University of Wisconsin-Madison , Madison, WI 53706 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,8]]},"reference":[{"key":"2026091415515897400_ref1","doi-asserted-by":"publisher","first-page":"1213361","DOI":"10.3389\/fendo.2023.1213361","article-title":"Molecular insights into mineralotropic hormone inter-regulation","volume":"14","author":"Pike","year":"2023","journal-title":"Front Endocrinol"},{"key":"2026091415515897400_ref2","doi-asserted-by":"publisher","first-page":"101775","DOI":"10.1016\/j.animal.2026.101775","article-title":"Review: genetic and physiological regulators of parathyroid hormone and the vitamin D system in pigs","volume":"6","author":"Oster","year":"2026","journal-title":"Animal."},{"issue":"6","key":"2026091415515897400_ref3","doi-asserted-by":"publisher","first-page":"1565","DOI":"10.1016\/j.bone.2007.01.017","article-title":"Mineralized tissue cells are a principal source of FGF23","volume":"40","author":"Yoshiko","year":"2007","journal-title":"Bone."},{"issue":"4","key":"2026091415515897400_ref4","doi-asserted-by":"publisher","first-page":"2543","DOI":"10.1074\/jbc.M408903200","article-title":"Circulating FGF-23 is regulated by 1alpha,25-dihydroxyvitamin D3 and phosphorus in vivo","volume":"280","author":"Saito","year":"2005","journal-title":"J Biol Chem"},{"issue":"6","key":"2026091415515897400_ref5","doi-asserted-by":"publisher","first-page":"G1036","DOI":"10.1152\/ajpgi.00243.2005","article-title":"1alpha,25-Dihydroxyvitamin D3 upregulates FGF23 gene expression in bone: the final link in a renal-gastrointestinal-skeletal axis that controls phosphate transport","volume":"289","author":"Kolek","year":"2005","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"issue":"4","key":"2026091415515897400_ref6","doi-asserted-by":"publisher","first-page":"F882","DOI":"10.1152\/ajprenal.00360.2010","article-title":"PTH increases FGF23 gene expression and mediates the high-FGF23 levels of experimental kidney failure: a bone parathyroid feedback loop","volume":"299","author":"Lavi-Moshayoff","year":"2010","journal-title":"Am J Physiol Renal Physiol"},{"issue":"27","key":"2026091415515897400_ref7","doi-asserted-by":"publisher","first-page":"18370","DOI":"10.1074\/jbc.M602469200","article-title":"Polypeptide GalNAc-transferase T3 and familial tumoral calcinosis. 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