{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T15:09:42Z","timestamp":1788188982378,"version":"build-2803163510"},"reference-count":18,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2024,6,29]],"date-time":"2024-06-29T00:00:00Z","timestamp":1719619200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"ORIC-FCCU","award":["IRIF-17"],"award-info":[{"award-number":["IRIF-17"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A comprehensive and systematic examination of dengue virus (DENV) evolution is essential in Pakistan, where the virus poses a significant public health challenge due to its ability to adapt and evolve. To shed light on the intricate evolutionary patterns of all four DENV serotypes, we analyzed complete genome sequences (n\u2005=\u200543) and Envelope (E) gene sequences (n\u2005=\u200544) of all four DENV serotypes collected in Pakistan from 1994 to 2023, providing a holistic view of their genetic evolution. Our findings revealed that all four serotypes of DENV co-circulate in Pakistan with a close evolutionary relationship between DENV-1 and DENV-3. Among the genetically distinct serotypes DENV-2 and DENV-4, DENV-4 stands out as the most genetically different, while DENV-2 exhibits greater complexity due to the presence of multiple genotypes and the possibility of temporal fluctuations in genotype prevalence. Selective pressure analysis of the Envelope (E) gene revealed heterogeneity among sequences (n\u2005=\u200544), highlighting 46 codons in the genome experiencing selective pressure, characterized by a bias toward balancing selection, indicating genetic stability of the virus. Furthermore, our study suggested an intriguing evolutionary shift of DENV-4 toward the DENV-2 clade, potentially influenced by antibodies with cross-reactivity to multiple serotypes, providing a critical insight into the complex factors, shaping DENV evolution and contributing to the emergence of new serotypes.<\/jats:p>","DOI":"10.1093\/jeb\/voae076","type":"journal-article","created":{"date-parts":[[2024,6,29]],"date-time":"2024-06-29T05:11:30Z","timestamp":1719637890000},"page":"915-925","source":"Crossref","is-referenced-by-count":5,"title":["Evolutionary patterns and heterogeneity of dengue virus serotypes in Pakistan"],"prefix":"10.1093","volume":"37","author":[{"given":"Zilwa","family":"Mumtaz","sequence":"first","affiliation":[{"name":"KAM School of Life Sciences, Forman Christian College University , Lahore , Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeeda","family":"Zia","sequence":"additional","affiliation":[{"name":"Department of Sciences and Humanities, National University of Computer and Emerging Sciences , Lahore , Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rashid","family":"Saif","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Qarshi University , Lahore , Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Farhan Ul Haque","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of the Punjab , Lahore , Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5087-7236","authenticated-orcid":false,"given":"Muhammad Zubair","family":"Yousaf","sequence":"additional","affiliation":[{"name":"KAM School of Life Sciences, Forman Christian College University , Lahore , 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