{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T12:17:39Z","timestamp":1790252259027,"version":"4.1.0"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T00:00:00Z","timestamp":1693440000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100019091","name":"Key Research and Development Program of Hunan Province of China","doi-asserted-by":"publisher","award":["2020NK2034"],"award-info":[{"award-number":["2020NK2034"]}],"id":[{"id":"10.13039\/501100019091","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Double first-class construction project of Hunan Agricultural University","award":["SYL2019029"],"award-info":[{"award-number":["SYL2019029"]}]},{"name":"Science and Technology Innovation Program of Hunan Province","award":["2022RC1025"],"award-info":[{"award-number":["2022RC1025"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,10,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The fall armyworm, Spodoptera frugiperda, is an invasive agricultural pest that is a serious threat to agricultural production and global food security. Chemical control is the most effective method for preventing outbreaks of S. frugiperda. However, insecticide resistance often develops as a result of prolonged pesticide use, and the molecular mechanisms involved in insecticide resistance remain unclear. Insect cytochrome P450 monooxygenases play an important role in the detoxification of insecticides and insecticide resistance in Lepidoptera. In our study, the LC50 of a novel insecticide (cyproflanilide) and a conventional insecticide (emamectin benzoate) for S. frugiperda second-instar larvae were 7.04 and 1.61 mg\/L, respectively. Furthermore, CYP321A9 expression was upregulated in larvae exposed to these insecticides. Additionally, knockdown of CYP321A9 by feeding larvae with dsRNA for 72 h significantly increased the mortality of S. frugiperda exposed to emamectin benzoate and cyproflanilide by 23.33% and 7.78%, respectively. Our results indicate that CYP321A9 may play an important role in the detoxification of emamectin benzoate and cyproflanilide in S. frugiperda. Our findings provide a basis to better understand the mechanisms of insecticide resistance and contribute to the control of S. frugiperda.<\/jats:p>","DOI":"10.1093\/jee\/toad168","type":"journal-article","created":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T08:36:48Z","timestamp":1692175008000},"page":"1812-1819","source":"Crossref","is-referenced-by-count":16,"title":["Function analysis of\n                    <i>CYP321A9<\/i>\n                    from\n                    <i>Spodoptera frugiperda<\/i>\n                    (Lepidoptera: Noctuidae) associated with emamectin benzoate, and a novel insecticide, cyproflanilide detoxification"],"prefix":"10.1093","volume":"116","author":[{"given":"Yang","family":"Shi","sequence":"first","affiliation":[{"name":"Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University , Changsha 410128 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"He","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University , Changsha 410128 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbing","family":"Ding","sequence":"additional","affiliation":[{"name":"National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University , Changsha 410128 , China"},{"name":"Hunan Provincial Engineering & Technology Research Center for Biopesticide and Formulation Processing, Hunan Agricultural University , Changsha 410128 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Huang","sequence":"additional","affiliation":[{"name":"Hunan Institute of Plant Protection, Hunan Academy of Agricultural Sciences , Changsha 410125 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hualiang","family":"He","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University , Changsha 410128 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Xue","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University , Changsha 410128 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiao","family":"Gao","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University , Changsha 410128 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhixiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Plant Protection, South China Agricultural University , Guangzhou 510642 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1774-3575","authenticated-orcid":false,"given":"Youzhi","family":"Li","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University , Changsha 410128 , China"},{"name":"National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University , Changsha 410128 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3920-0950","authenticated-orcid":false,"given":"Lin","family":"Qiu","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University , Changsha 410128 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,8,31]]},"reference":[{"key":"2023101018115648600_CIT0001","first-page":"265","article-title":"A method of computing the effectiveness of an insecticide","volume-title":"J Econ Entomol","author":"Abbott","year":"1925"},{"key":"2023101018115648600_CIT0002","first-page":"1217.28","article-title":"The calculation of LD50 using Probit analysis","volume":"27","author":"Akay","year":"2013","journal-title":"FASEB 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